Isolation of single chain antibody fragments with specificity for cell surface antigens by phage display utilizing internal image anti-idiotypic antibodies

Isolation of Single Chain Variable Fragments against Six Esters of Pyrethrins by Subtractive Phage Display

“…Monoclonal antibodies are now considered an epochal breakthrough in medicine and are suitable as experimental tools, diagnostic reagents in therapeutics. 26,27) The advantage of phage display demonstrates a rapid and efficient strategy to generate antigen-specific monoclonal antibodies, surpassing hybridoma technology. Herein, we present the results of the library construction and selection of phage display recombinant mAbs derived from pyrethrinimmunized mice.…”

Simultaneous targeting of IL2 and IL12 to Hodgkin's lymphoma cells enhances activation of resting NK cells and tumor cell lysis

“…Generation and purification of the CD30-specific single-chain antibody HRS3 scFv and the recombinant CD30-human Fc fusion protein were described earlier. [10][11][12] The anti-HRS3 idiotypic mAb 9G10 and the recombinant CD30-human Fc fusion protein were previously described.…”

“…9 To counteract the Th2 polarization in Hodgkin's disease, we generated 2 recombinant anti-CD30 antibody-cytokine fusion proteins to target IL2 and IL12 to CD30 þ H/RS cells. The antibody-cytokine fusion proteins consist of the anti-CD30 single-chain fragment (scFv) antibody HRS3 10 that is linked either to the N-terminus of IL2 or to the p40 subunit of single-chain IL12. Here we demonstrate that both the anti-CD30-scFv-IL2 and the anti-CD30-scFv-IL12 fusion protein bind specifically to CD30 and retained functional cytokine activity in vitro, i.e., activation of resting NK cells to lysis of CD30 þ Hodgkin's lymphoma cells.…”

Single‐Chain Fv Fragments from Phage Display Libraries

“…ScFvs can not only be isolated against antigen but also using monoclonal antiidiotypic antibodies, which mimic cell surface receptors. The feasibility of this approach was demonstrated in a model system with an anti-CD30 scFv and antiidiotypic monoclonal antibodies that carry an internal image of an epitope of membrane-bound CD30, an antigen overexpressed on Hodgkin's lymphoma cells (Hombach et al, 1998).…”