Isolation of spore-forming bacilli from mosquitoes in natural breeding habitats in Iraq

Mohsen, Zohair H.; Ibrahim, M.; Al-Jadooa, N. S.

doi:10.1007/bf02372370

Cited by 3 publications

(7 citation statements)

References 8 publications

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“…Bacillus sphaericus (H6) was first isolated by Yousten et al . (1980 ) from the Philippines and was then further isolated from various parts of the world ( Anonymous 1996), including Iraq ( Mohsen et al . 1986 ).…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus cereus , the dominant bacterium in our isolates, has no parasporal body and has been isolated from a variety of insect hosts, i.e. Lepidoptera, Hymenoptera, Coleoptera and Diptera ( Krieg 1981 ; Mohsen et al . 1986 , 1989).…”

Section: Discussionmentioning

confidence: 99%

“…1986 , 1989). Mohsen et al . (1986 , 1989) isolated B. cereus from dead pupae of C. longiareolata , dead larvae of Culex quinquefasciatus Say, soil and mud samples in Iraq.…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus sphaericus (H6) was first isolated by Yousten et al (1980) from the Philippines and was then further isolated from various parts of the world (Anon. 1996), including Iraq (Mohsen et al 1986). This bacterium is considered to be one of the most toxic strains of B. sphaericus for mosquitoes (de Barjac et al 1988).…”

Section: Discussionmentioning

confidence: 99%

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Isolation of endospore‐forming bacilli toxic toCuliseta longiareolata(Diptera : Culicidae) in Jordan

Khyami-Horani¹,

Katbeh‐Bader

Mohsen

1999

Letters in Applied Microbiology

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Ten of 80 endospore‐forming bacilli, isolated from various habitats of Jordan, were found to be highly toxic to the 4th instar larvae of Culiseta longiareolata (Macquart). The bacilli were identified into the following species and strains : Bacillus sphaericus (H6), B. sphaericus (H9a, 9b), B. cereus Frankland and Frankland, B. brevis Migula and B. megaterium Bary. Bacillus cereus comprised 50% of the isolates. The toxic concentrations of these isolates against C. longiareolata ranged between 1·2 × 107 and 1·1 × 109 viable spores ml−1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…1986 , 1989). Mohsen et al . (1986 , 1989) isolated B. cereus from dead pupae of C. longiareolata , dead larvae of Culex quinquefasciatus Say, soil and mud samples in Iraq.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Isolation of endospore‐forming bacilli toxic toCuliseta longiareolata(Diptera : Culicidae) in Jordan

Khyami-Horani¹,

Katbeh‐Bader

Mohsen

1999

Letters in Applied Microbiology

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“…products as biopesticide inspired the work to pursue this research of isolation and characterization of Bacillus thuringiensis from mosquito breeding sites. This study presents information on the occurrence of spore forming bacilli bacteria in the bodies of mosquito larvae as well as in the soil of their natural breeding sites (Mohsen et al, 1986). The results show that Bacillus thuringiensis strains are found in mosquito breeding sites.…”

Section: Discussionmentioning

confidence: 96%

Quick isolation and characterization of novel Bacillus<br>thuringiensis strains from mosquito breeding sites in Malaysia

Renganathan¹,

Rathinam²,

Danial³

et al. 2011

Emir. J. Food Agric

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Bacillus thuringiensis (Bt.) is an insecticidal, gram-positive and spore forming bacterium which has capabilities infecting the insect vectors belonging to the order Diptera (mosquitoes) especially dengue causing Aedes species. Bacterial strains with combination of Cry and Cyt proteins are useful in controlling and management of resistance in mosquito population. The objective of this research is to isolate and characterize Bacillus thuringiensis from mosquito breeding sites and also study expression of Crystal (Cry) and Cytolytic (Cyt) proteins of these isolates by SDS-PAGE analysis. In this study, 50 samples were collected from mosquito breeding sites which include soil, larvae and water. Samples were processed by initially giving heat shock treatment, serial dilution and followed by plating onto culture medium. Initial selection was based on colony morphology, gram staining, phase contrast microscopy and coomassie blue staining. Total of 144 isolates was identified based on colony morphology and this number was reduced to 69 after gram staining. Total of 15 isolates were confirmed to be Bacillus after phase contrast microscopy. Through coomassie blue staining, a blue cap-like appearance in the sporulation phase and darkly stained oval shaped candidate in autolysis phase can be observed. Growth curve analysis was done with one chosen isolate to determine the generation time. Further protein level characterization based on the SDS-PAGE gel showed the banding patterns range from 65-274 kDa (Cry proteins) and 21-25 kDa (Cyt proteins). Keywords: Bacillus thuringiensis,

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Toxicity of a Brevibacillus laterosporus strain lacking parasporal crystals against Musca domestica and Aedes aegypti

2007

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Isolation of spore-forming bacilli from mosquitoes in natural breeding habitats in Iraq

Abstract: New isolates of spore -forming bacilli from larvae and pupae of 3 species of mosquitoes are recorded in central Iraq.

Cited by 3 publications

References 8 publications

Isolation of endospore‐forming bacilli toxic toCuliseta longiareolata(Diptera : Culicidae) in Jordan

Isolation of endospore‐forming bacilli toxic toCuliseta longiareolata(Diptera : Culicidae) in Jordan

Quick isolation and characterization of novel Bacillus<br>thuringiensis strains from mosquito breeding sites in Malaysia

Toxicity of a Brevibacillus laterosporus strain lacking parasporal crystals against Musca domestica and Aedes aegypti

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