Isolation of subsp. (LC variant, Y-Goat) from naturally aborted bovine fetuses

Kapoor, Prerna; Garg, D. N.; Mahajan, S. K.

doi:10.1016/0093-691x(89)90289-6

Cited by 19 publications

(7 citation statements)

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“…Mmm has been isolated from sheep and goats [ 14 ] however there is no indication that it can cause disease in these species. Likewise, Mmc has also been isolated from cattle [ 15 ], but has not been reported to cause disease in cattle.…”

Section: Introductionmentioning

confidence: 99%

Cyto-adherence of Mycoplasma mycoides subsp. mycoides to bovine lung epithelial cells

et al. 2015

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BackgroundMycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a respiratory disease of cattle, whereas the closely related Mycoplasma mycoides subsp. capri (Mmc) is a goat pathogen. Cyto-adherence is a crucial step in host colonization by mycoplasmas and subsequent pathogenesis. The aim of this study was to investigate the interactions between Mmm and mammalian host cells by establishing a cyto-adherence flow cytometric assay and comparing tissue and species specificity of Mmm and Mmc strains.ResultsThere were little significant differences in the adherence patterns of eight different Mmm strains to adult bovine lung epithelial cells. However, there was statistically significant variation in binding to different host cells types. Highest binding was observed with lung epithelial cells, intermediate binding with endothelial cells and very low binding with fibroblasts, suggesting the presence of effective adherence of Mmm on cells lining the airways of the lung, which is the target organ for this pathogen, possibly by high expression of a specific receptor. However, binding to bovine fetal lung epithelial cells was comparably low; suggesting that the lack of severe pulmonary disease seen in many infected young calves can be explained by reduced expression of a specific receptor.ConclusionsMmm bound with high efficiency to adult bovine lung cells and less efficiently to calves or goat lung cells. The data show that cyto-adherence of Mmm is species- and tissue- specific confirming its role in colonization of the target host and subsequent infection and development of CBPP.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0347-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Cyto-adherence of Mycoplasma mycoides subsp. mycoides to bovine lung epithelial cells

et al. 2015

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“…24,30 Conversely, LC types are almost exclusively found in goats but have been recovered from cattle in Australia, 30 France, 88 and India. 63 In 1 instance this mycoplasma has been associated with natural disease in sheep in Nigeria. 87 Mycoplasma m. ssp.…”

Section: Mycoplasma Mycoides Ssp Mycoides (Large-colony or Caprine Bmentioning

confidence: 99%

Mycoplasmas of Goats and Sheep

1992

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“…To date, direct evidence that supports the association of swine mycoplasmas with abortion has not been documented, although abortion-related mycoplasmas have been reported in other species such as bovine [9], caprine [24], equine [17], or feline [30]. Further studies would be necessary to determine whether M. hyorhinis plays a pathogenetic role in abortion-related problems of pregnant gilts or sows.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Cytopathogenic Mycoplasma hyorhinis from Swine Farms with a History of Abortions

et al. 2003

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ABSTRACT. A virus-like cytopathic agent isolated from swine farms with a history of recurrent abortion episodes was investigated. We employed a differential display reverse transcription-polymerase chain reaction (ddRT-PCR) to obtain genetic information of the cytopathic agent. Partial nucleotide sequence (527 bp) obtained from differentially displayed PCR fragments showed 88.7% similarity with the 23S rRNA gene of Mycoplasma hyopneumoniae. Unexpectedly, the 5' portion (1-333 bp) of the sequence shared 96.1% similarity with 5' untranslated region (UTR) of human prostate tumor inducing gene 1 (PTI-1). Cytopathic effects and extranuclear DNA fluorescence were no longer observed when BM-cyclin was added in the culture medium, suggesting that BM-cyclin sensitive mycoplasmalike organisms caused the cell death. Further evidence supporting the cytopathic agent as a mycoplasma-like organism was obtained by the capability of 3 H-thymidine and 3 H-uridine incorporation, a single peak in buoyant density gradient profile (1.20-1.24 g/ml), and ultrastructural morphology. Unlike M. hyopneumoniae, the organism was not propagated in Friis medium. Nucleotide sequence of 16S rRNA obtained from the cytopathic agent showed 0.8-1.0% divergences with other M. hyorhinis strains, suggesting that the newly isolated cytopathogenic swine mycoplasma was a variant form of M. hyorhinis. Striking homology between a portion of the 23S rRNA gene of M. hyorhinis and 5' UTR of human PTI-1 implicated that M. hyorhinis might potentially be related to the evolution of human PTI-1.

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Isolation of subsp. (LC variant, Y-Goat) from naturally aborted bovine fetuses

Cited by 19 publications

References 6 publications

Cyto-adherence of Mycoplasma mycoides subsp. mycoides to bovine lung epithelial cells

Cyto-adherence of Mycoplasma mycoides subsp. mycoides to bovine lung epithelial cells

Mycoplasmas of Goats and Sheep

Identification and Characterization of Cytopathogenic Mycoplasma hyorhinis from Swine Farms with a History of Abortions

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