ISOLATION OF THE FIRST PISCINE TRANSFORMING GROWTH FACTOR Β GENE: ANALYSIS REVEALS TISSUE SPECIFIC EXPRESSION AND a POTENTIAL REGULATORY SEQUENCE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Hardie, Laura J.; Laing, Kerry J.; Daniels, Garry D.; Grabowski, Peter; Cunningham, Charles; Secombes, Christopher J.

doi:10.1006/cyto.1997.0334

Cited by 104 publications

(60 citation statements)

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“…This allowed the design of gene-specific primers to confirm and extend these results. Initial PCR using CXC-R forward (F) 1 (5Ј-GTGCAtGTGATCTACACCATC-3Ј) and CXC-R reverse (R) 1 (5Ј-GAGCTGTGGCAAACACTATGT-3Ј) primers was performed on an O. mykiss macrophage-enriched head-kidney leukocyte cDNA library, prepared as described previously [21], and gave a product of 199 bp, confirming the presence of the gene. Subsequently, anchored PCR using the gene-specific primer CXC-R R1 with the vector-specific primer T3 (5Ј-AATTAACCCTCAC-TAAAGGG-3Ј) was used to obtain the 5Ј end of the gene, whereas nested PCR with CXC-R F1 and vector-specific primer T7 (5Ј-GTAATACGACTCAC-TATAGGGC-3Ј) followed by reamplification with CXC-R F2 (5Ј-TGGCTACCTGCTGtTaTTCTC-3Ј) and T7 was used to obtain the 3Ј end (Fig.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…Poly(A) mRNA was isolated as previously described [21] from rainbow trout head-kidney leukocytes, gill, brain, spleen, liver, and blood. Extracted RNA was treated with DNase I (Pharmacia Biotech) to ensure that genomic DNA was not amplified during PCR.…”

Section: Tissue-specific Distribution Of Trout Chemokine Receptormentioning

confidence: 99%

“…Extracted RNA was treated with DNase I (Pharmacia Biotech) to ensure that genomic DNA was not amplified during PCR. First-strand cDNA was synthesized as previously described [21] and used in reverse transcriptase (RT)-PCR with the primers CXC-R F1/R1 or CC-R F5 (5Ј-TGTGACAAATCTGCCGTTAG-3Ј)/R4 (5Ј-GCTCCTTGACGTTGGCGAACATG-3Ј). Standard PCR reaction conditions and thermal cycling protocol were used as described in Section 2.1.…”

Section: Tissue-specific Distribution Of Trout Chemokine Receptormentioning

confidence: 99%

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Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

Daniels

Zou

Charlemagne

et al. 1999

Journal of Leukocyte Biology

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Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Section: Tissue-specific Distribution Of Trout Chemokine Receptormentioning

confidence: 99%

Section: Tissue-specific Distribution Of Trout Chemokine Receptormentioning

confidence: 99%

See 1 more Smart Citation

Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

Daniels

Zou

Charlemagne

et al. 1999

Journal of Leukocyte Biology

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“…Then, they were denatured, neutralized and transferred to nitrocellulose membrane, and were bound to the membrane by baking at 75 for 2 h. Virtual Northern blotting was also performed as described in dot blotting using the same Detection Starter Kit I. and different phases of oocytes were isolated from one-year old silver crucian carp using Trizol reagent (GIBCO). The RNAs were incubated with DNase I (RNase-free, Boehringer Mannheim) and then were reverse-transcribed with SuperScript II Reverse Transcriptase (GIBRCO) and oligo(dT) [8][9][10][11][12] . The first strand cDNAs were used as templates for PCR with the upstream and downstream primers.…”

Section: Virtual Northern Blotting To Verify Differential Expressionmentioning

confidence: 99%

“…Growth factors IGF-I and TGF-β are synthesized in specific stage and are thought to exert important functions in regulating oocyte growth and development [9,10] . Although TGF-β cDNA has been cloned in fish [11] , its function is still not known. Indirect evidence indicated that IGF in teleost fish has similar roles to that in mammal [12] .…”

mentioning

confidence: 99%

Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

Wen

Xie

Liu

et al. 2001

Sci. China Ser. C.-Life Sci.

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A new gene with WD domains is cloned and characterized according to its differential transcription and expression between previtellogenic oocytes (phase I oocytes) and fully-grown oocytes (phase V oocytes) from natural gynogenetic silver crucian carp (Carassius auratus gibelio) by using the combinative methods of suppressive subtraction hybridization, SMART cDNA synthesis and RACE-PCR. The full-length cDNA is 1870 bp. Its 5 untranslated region is 210 bp, followed by an open reading frame of 990 bp, which has the typical vertebrate initiator codon of AN-NATG. The open reading frame encodes a protein with 329 amino acids. It has 670 bp of 3 untranslated region and an AATAAA polyadenylation signal. Because it has 92% homology to STRAP (serine-threonine kinase receptor-associated protein), a recently reported gene, we named it FSTRAP (fish STRAP). Virtual Northern blotting indicated that the FSTRAP was transcribed in fully-grown oocytes (phase V oocytes), but not in previtellogenic oocytes (phase I oocytes). RT-PCR analysis showed that FSTRAP was transcribed in brain, heart, kidney, muscle, ovary, spleen and testis, but not in liver. And its mRNA could be detected in the oocytes from phase II to phase V. Western blotting also showed that FSTRAP protein could be detected in brain, heart, kidney, muscle, ovary, spleen and testis except liver. Results of Western blotting on various oocytes were also similar to the RT-PCR data. FSTRAP protein was not expressed in the previtellogenic oocytes. Its expression initiated from phase II oocytes after vitellogenesis, and was consistent with the mRNA transcription. The studies on functional roles of genetic factors and maternal RNAs in oogenesis have been mainly focused on the human being and cultured animals. In fish, however, only a few of recent attempts have been made to identify the genetic factors [1] , but the knowledge about regulative mechanism underlying gene transcription and translation in oogenesis is very little. Recently, along with advances of developmental biology, fish oogenesis has been recognized as an important research field. For instance, Selman et al. investigated different stages of oocyte development in zebrafish (Brachydanio rerio) [2] . Tyler and Sumpter reviewed oocyte growth and development in teleosts [3] . Nagahama et al. initiated the studies on molecular biology of oocyte growth and maturation in fish [4] . However, numerous basic questions in fish oogenesis remain unanswered.

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Spermatogonia of rainbow trout: II. in vitro study of the influence of pituitary hormones, growth factors and steroids on mitotic activity

Loir¹

1999

Mol. Reprod. Dev.

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At the present time, in spite of recent advances, knowledge about the factors regulating germ cell proliferation in the teleost testis is limited. This study was designed to investigate, in vitro, the ability of various hormones, growth factors, and steroids to influence the proliferation of trout spermatogonia (Go) present in mixed cultures of somatic and germ cells prepared from testes, either prespermatogenetic or spermatogenetic. The tested molecules were usually present for the duration of culture (4.5 days) and 3H‐thymidine (3H‐Tdr) for the last day in culture. In our cell culture conditions, homologous gonadotropin I (tGTH‐I) and growth hormone (tGH) moderately stimulated 3H‐Tdr incorporation by Go, with ED50 equal to 5.5 ± 3.0 and 1.8 ± 0.4 ng/ml respectively. Insulin growth factor I (rhIGF‐I) and fibroblast growth factor (rhFGF‐2) stimulated 3H‐Tdr incorporation by Go from spermatogenetic testes only, with ED50 equal to 16.2 ± 9.3 and 2.4 ± 0.3 ng/ml respectively. The effects of the most efficient concentrations of rhIGF‐I combined with those of either tGTH‐I or tGH were additive. Seventy to one hundred μM suramin stimulated 3H‐Tdr incorporation by Go from testes at all maturation stages and this effect was additive with that of tGTH‐I. We assume that this effect of suramin could result from the inhibition of an unidentified antimitogenic factor. No effect was observed with homologous prolactin, human epidermal growth factor, activin A and B, transforming growth factor‐β1, testosterone, 11‐ketotestosterone, 17β‐estradiol, pregnenolone, 11β‐hydroxyprogesterone, and 22‐hydroxycholesterol. In conclusion, our in vitro results suggest that GTH‐I, GH, IGF‐I, and FGF‐2, are potent in situ modulators of the proliferative activity of trout Go at the time of induction, speeding up, then slowing down spermatogenesis, through direct or indirect additive and/or antagonistic influences. Mol. Reprod. Dev. 53:434–442, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

ISOLATION OF THE FIRST PISCINE TRANSFORMING GROWTH FACTOR Β GENE: ANALYSIS REVEALS TISSUE SPECIFIC EXPRESSION AND a POTENTIAL REGULATORY SEQUENCE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Cited by 104 publications

References 0 publications

Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

Differential expression and characterization analysis of a new gene with WD domains in fish oogenesis

Spermatogonia of rainbow trout: II. in vitro study of the influence of pituitary hormones, growth factors and steroids on mitotic activity

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