Isolation of the β-tubulin gene and characterization of thiabendazole resistance in<i>Gibberella pulicaris</i>

Kawchuk, L. M.; Hutchison, Leonard J.; Verhaeghe, Catherine; Lynch, D. R.; Bains, P. S.; Holley, J.

doi:10.1080/07060660309507001

Cited by 37 publications

(16 citation statements)

References 19 publications

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“…In contrast to laboratory mutants, most field isolates of plant-pathogenic fungi exhibit codon changes that, strikingly, seem to be restricted to positions 50 (22), 198, 200 (1,13), and 240 (1). Only a few exceptions of mutations at these positions have been reported for isolates of Venturia inaequalis, Penicillium expansum, and Penicillium aurantiogriseum with low levels of resistance to benomyl (13) and thiabendazole-resistant isolates of Gibberella pulicaris (12), but the exact molecular mechanisms for their resistance have not yet been determined.…”

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confidence: 99%

Identification and Characterization of BenzimidazoleResistance in Monilinia fructicola from Stone Fruit OrchardsinCalifornia

Yoshimura

Michailides

2003

Appl Environ Microbiol

181

101

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Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit. Isolates that had low levels of resistance (hereafter referred to as LR isolates) and high levels of resistance (hereafter referred to as HR isolates) were also cold and heat sensitive, respectively. Results from microsatellite DNA fingerprints showed that genetic identities among the populations of sensitive (S), LR, and HR isolates were very high (>0.96). Analysis of DNA sequences of the ␤-tubulin gene showed that the LR isolates had a point mutation at codon 6, causing a replacement of the amino acid histidine by tyrosine. Codon 198, which encodes a glutamic acid in S and LR isolates, was converted to a codon for alanine in HR isolates. Based on these point mutations in the ␤-tubulin gene, allele-specific PCR assays were developed for rapid detection of benzimidazoleresistant isolates of M. fructicola from stone fruit.

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confidence: 99%

Identification and Characterization of BenzimidazoleResistance in Monilinia fructicola from Stone Fruit OrchardsinCalifornia

Yoshimura

Michailides

2003

Appl Environ Microbiol

181

101

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“…Sacc.) (Kawchuk et al 2002) and F. graminearum (Li et al 2003). In contrast to Fusarium species, thiophanate-methylresistant isolates of M. nivale had a mutation at codon 198 in the b-tubulin gene.…”

Section: Discussionmentioning

confidence: 97%

Genetic analysis and PCR-based identification of major Fusarium species causing head blight on wheat in Japan

et al. 2008

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“…Most field resistant isolates of plant pathogenic fungi show codon changes that seem to be restricted to positions 50 (McKay et al 1998), 198, 200 (Albertini et al 1999Koenraadt et al 1992), and 240 (Albertini et al 1999). Only a few exceptions to these point mutations have been reported in Venturia inaequalis, Penicillium expansum, Penicillium aurantiogriseum (Koenraadt et al 1992), Gibberella pulicaris (Kawchuk et al 2002), and Gibberella zeae (Chen et al 2005), but the exact molecular mechanisms for their resistances have not been clarified.…”

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confidence: 95%

Detection and characterization of benzimidazole resistance of Botrytis cinerea in greenhouse vegetables

Zhang

Liu²,

Zhu

2009

Eur J Plant Pathol

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From 2003 to 2006, a total of 426 singleconidial isolates of B. cinerea collected from greenhouse vegetables in China were characterized for resistance to benzimidazole fungicides and diethofencarb according to inhibition of mycelial growth. Rapid development of double-resistance to benzimidazoles and diethofencarb was observed. Three types of benzimidazole-resistant isolates, Ben R1, Ben R2 and Ben R3 were detected. A new phenotype, Ben R3, which showed low level of resistance to benzimidazole fungicides and resistance to diethofencarb, was detected with frequencies of 6.8%, 10.0%, 13.2% and 12.4% from 2003 to 2006, respectively. Further studies indicated that Ben R3 was caused by a point mutation from GAG in sensitive(S) isolates to GTG at codon 198 in the β-tubulin gene, predicted to cause a change from glutamic acid to valine. Ben R3 isolates had comparable growth, sporulation and pathogenicity ability as isolates of other phenotypes but were more sensitive at lower temperatures.

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Isolation of the β-tubulin gene and characterization of thiabendazole resistance inGibberella pulicaris

Cited by 37 publications

References 19 publications

Identification and Characterization of BenzimidazoleResistance in Monilinia fructicola from Stone Fruit OrchardsinCalifornia

Identification and Characterization of BenzimidazoleResistance in Monilinia fructicola from Stone Fruit OrchardsinCalifornia

Genetic analysis and PCR-based identification of major Fusarium species causing head blight on wheat in Japan

Detection and characterization of benzimidazole resistance of Botrytis cinerea in greenhouse vegetables

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