Isolation of Trichoderma harzianum and Evaluation of Antagonistic Potential against Alternaria alternata

Chaitanya, K.; Masih, Harison; Abhiram, Pakkala

doi:10.20546/ijcmas.2018.710.338

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…Excess dye solution was absorbed with absorbent paper, and the glass slides were baked and fixed with an alcohol burner, then placed under the microscope for hyphal observation. Additional hyphae were picked and placed in an electron microscopy fixing solution for 12 h, then sent to Wuhan Servicebio Technology Co., Ltd. (Wuhan, China) for SEM sample observation [53].…”

Section: Microscopy and Electron Microscopy Observationsmentioning

confidence: 99%

The Endophytic Strain Trichoderma asperellum 6S-2: An Efficient Biocontrol Agent against Apple Replant Disease in China and a Potential Plant-Growth-Promoting Fungus

Wang

Zhang

Duan

et al. 2021

JoF

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A study was conducted for endophytic antagonistic fungi obtained from the roots of healthy apple trees growing in nine replanted orchards in Shandong Province, China. The fungi were assessed for their ability to inhibit Fusarium proliferatum f. sp. malus domestica MR5, a fungal strain associated with apple replant disease (ARD). An effective endophyte, designated as strain 6S-2, was isolated and identified as Trichoderma asperellum. Strain 6S-2 demonstrated protease, amylase, cellulase, and laccase activities, which are important for the parasitic and antagonistic functions of pathogenic fungi. The inhibition rate of 6S-2 against Fusarium proliferatum f. sp. malus domestica MR5 was 52.41%. Strain 6S-2 also secreted iron carriers, auxin, ammonia and was able to solubilize phosphorus. Its fermentation extract and volatile substances inhibited the growth of MR5, causing its hyphae to twist, shrink, swell, and rupture. The antifungal activity of the 6S-2 fermentation extract increased with increasing concentrations. It promoted the production and elongation of Arabidopsis thaliana lateral roots, and the strongest effects were seen at a concentration of 50 mg/mL. A GC-MS analysis of the 6S-2 fermentation extract and volatile substances showed that they comprised mainly alkanes, alcohols, and furanones, as well as the specific volatile substance 6-PP. The application of 6S-2 spore suspension to replanted apple orchard soils reduced plant oxidative damage and promoted plant growth in a pot experiment. Therefore, the endophytic strain T. asperellum 6S-2 has the potential to serve as an effective biocontrol fungus for the prevention of ARD in China, and appears to promote plant growth.

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Section: Microscopy and Electron Microscopy Observationsmentioning

confidence: 99%

The Endophytic Strain Trichoderma asperellum 6S-2: An Efficient Biocontrol Agent against Apple Replant Disease in China and a Potential Plant-Growth-Promoting Fungus

Wang

Zhang

Duan

et al. 2021

JoF

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Quantitative and qualitative analysis of bioaerosols emissions from the domestic eastern wastewater treatment plant, Alexandria, Egypt

El-Bestawy,

Ibrahim,

Shalaby

2024

Sci Rep

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Bioaerosol studies showed that wastewater treatment plants (WWTPs) are a significant source of bioaerosol emissions. In this study, 170 samples of total bacteria, total coliform, and total fungi were collected from 10 sites within a domestic WWTP, Alexandria, Egypt, using the sedimentation technique. According to the Index of Microbial Air Contamination (IMA) classes, the total bacteria range was 108–5120 CFU/dm2/hour, and all samples were classified as “very poor” except one sample of an office, which was classified as “poor.” The total coliform range was 0–565 CFU/dm2/hour, and 6 samples were classified as “very poor,” while one sample was classified as “poor.” The total fungi range was 0–209 CFU/dm2/hour, and 9 samples were classified as “very poor,” while 4 samples were classified as “poor.” After the conversion to CFU/m3, the counts of total bacteria, total coliforms, and total fungi were 897 − 42.7 × 103, 0–4.71 × 103, and 0–2.69 × 103 CFU/m3, respectively. Several identified bioaerosols have been reported before as a cause of human infections. They included Lysinibacillus fusiformis, Bacillus cereus, Alcaligenes faecalis, Klebsiella sp., Escherichia coli, Aspergillus spp., Penicillium spp., Rhizopus sp., Candida sp., and Rhodotorula sp. These results indicated an increased health risk to WWTP staff, which needs more attention and more efficient control measures.

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Biocontrol efficacy of Meyerozyma guilliermondii LMA-Cp01 against post-harvest pathogens of fruits

López-Cruz,

Segarra,

Torres

et al. 2023

Archives of Phytopathology and Plant Protection

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This work aimed to broaden the spectrum biocontrol activity of Meyerozyma guilliermondii LMA-Cp01. Inhibition of postharvest pathogens, viz., Alternaria alternata, Penicillium italicum, P. digitatum, P. expansum, Botrytis cinerea, and Rhizopus stolonifer was tested on persimmon, oranges, apples, and pears. The effect of spraying or dipping application of the actinomycete for the phytopathogens biocontrol was evaluated. LMA-Cp01 reduced 30 and 50% (R. stolonifer), 53 and 67% (B. cinerea), and 55 and 67% (P. expansum) the disease incidence and severity on apples, respectively. Regarding pears, reductions of 44 and 71% (R. stolonifer) and 65 and 85% (P. expansum) of incidence and severity were respectively achieved. P. italicum and P. digitatum incidence on oranges was reduced by 70 and 72%, respectively, and the severity was also reduced by 85%. The LMA-Cp01 efficacy on apples and oranges was higher by spraying application. LMA-Cp01 is a promising broad-spectrum biocontrol agent against diseases on different hosts.

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Isolation of Trichoderma harzianum and Evaluation of Antagonistic Potential against Alternaria alternata

Cited by 3 publications

References 2 publications

The Endophytic Strain Trichoderma asperellum 6S-2: An Efficient Biocontrol Agent against Apple Replant Disease in China and a Potential Plant-Growth-Promoting Fungus

The Endophytic Strain Trichoderma asperellum 6S-2: An Efficient Biocontrol Agent against Apple Replant Disease in China and a Potential Plant-Growth-Promoting Fungus

Quantitative and qualitative analysis of bioaerosols emissions from the domestic eastern wastewater treatment plant, Alexandria, Egypt

Biocontrol efficacy of Meyerozyma guilliermondii LMA-Cp01 against post-harvest pathogens of fruits

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