Isolation of turkesterone from the epigeal part ofAjuga turkestanica and its anabolic activity

Mamatkhanov, A. U.; Yakubova, M. R.; Сыров, В. Н.

doi:10.1007/bf02249133

Cited by 13 publications

(9 citation statements)

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“…Traditionally, shoots and leaves are dried and steeped in hot water and the broth is ingested to treat bodily ailments such as heart disease, and muscle and stomach aches (Mamatkhanov et al 1998;Abdukadirov et al 2004;personal communication, Dr. B. Islamov, Samarkand State University). A. turkestanica produces a rich array of bioactive phytochemicals including the phytoecdysteroids turkesterone, 20-hydroxyecdysone (20E; also reported as ecdysterone), cyasterone, cyasterone 22-acetate, ajugalactone, ajugasterone B, a-ecdysone and ecdysone 2,3-monoacetonide along with the iridoids harpegide and harpegide 8-acetate (Usmanov et al 1971(Usmanov et al , 1973(Usmanov et al , 1975(Usmanov et al , 1978Baltaev 2000;Ramazanov 2005).…”

Section: Introductionmentioning

confidence: 98%

In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

Cheng

Yousef

Grace

et al. 2008

Plant Cell Tiss Organ Cult

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In order to develop a sustainable source of metabolism-enhancing phytoecdysteroids, cell suspension and hairy root cultures were established from shoot cultures of wild-harvested Ajuga turkestanica, a medicinal plant indigenous to Uzbekistan. Precursors of phytoecdysteroids (acetate, mevalonic acid cholesterol) or methyl jasmonate (an elicitor) were added to subculture media to increase phytoecdysteroid accumulation. In cell suspension cultures, 20-hydroxyecdysone (20E) content increased 3-or 2-fold with the addition of 125 or 250 lM methyl jasmonate, respectively, compared to unelicited cultures. Precursor addition, however, did not provoke phytoecdysteroid accumulation. In hairy root cultures, addition of sodium acetate, mevalonic acid, and methyl jasmonate, but not cholesterol, increased phytoecdysteroid content compared to unelicited cultures. Hairy root cultures treated with 150 mg l -1 sodium acetate, or 15 or 150 mg l -1 mevalonic acid, increased 20E content approximately 2-fold to 19.9, 20.4 or 21.7 lg mg -1 , respectively, compared to control (10.5 lg mg -1 ). Older hairy root cultures, extracted after the seventh subculture cycle, also showed increases in 20E content (24.8 lg mg -1 ), turkesterone (0.9 lg mg -1 ) and cyasterone (8.1 lg mg -1 ) compared to control cultures maintained for a shorter duration of four subculture cycles. Doses of 10 or 20 lg ml -1 hairy root extract increased protein synthesis by 25.7% or 31.1%, respectively, in a C2C12 mouse skeletal cell line. These results suggest that sustainable production of metabolically active phytoecdysteroid can be achieved through hairy root culture systems. Keywords 20-Hydroxyecdysone Á Cell suspension culture Á Cyasterone Á Hairy root Á Methyl jasmonate Á Mevalonic acid Á Turkesterone Abbreviations 20E 20-Hydroxyecdysone CC Column chromatography CD 3 OD Deuterated methanol DAD Diode array detector DMEM Dulbecco's modified eagle's media DPM Decays per minute DW Dry weight ESI-MS Electrospray ionization-mass spectroscopy FW Fresh weight HPLC High performance liquid chromatography Electronic supplementary material The online version of this article (

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Section: Introductionmentioning

confidence: 98%

In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

Cheng

Yousef

Grace

et al. 2008

Plant Cell Tiss Organ Cult

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“…Ajuga turkestanica (Regel) Briq., a perennial herb growing mainly in Central Asia, is known as a rich source of bioactive substances and is used by local people to treat heart diseases, muscle and stomach aches (Mamatkhanov et al ., 1998; Abdukadirov et al ., 2004). Several phytoecdysteroids (turkesterone, 20-hydroxyecdysone, cyasterone, cyasterone 22-acetate, ajugalactone, ajugasterone B, α- ecdysone and ecdysone 2, 3-monoacetonide) have been reported in this species along with the iridoids harpagide and harpagide 8-acetate (Usmanov et al ., 1971, 1973, 1975, 1978; Baltaev, 2000; Ramazanov, 2005).…”

Section: Introductionmentioning

confidence: 99%

Neo-clerodane diterpenes from Ajuga turkestanica

Grace

Cheng

Raskin

et al. 2008

Phytochemistry Letters

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The ethyl acetate extract of the aerial parts of Ajuga turkestanica afforded 6 neo-clerodane diterpenes, including two novel compounds, 14, 15-dihydroajugachin B (1) and 14-hydro-15-methoxyajugachin B (2), in addition to the known diterpenoids chamaepitin (3), ajugachin B (4), ajugapitin (5) and lupulin A (6). Structures were established through exhaustive NMR spectroscopic analysis and chemical transformation in the case of 1. The full 1 H and 13 C NMR assignment of the C-15 R and S configurations of 14-hydro-15-methoxyajugachin B and chamaepitin were elucidated.

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“…Ecdysteroid‐enriched extracts of A . turkestanica (or pure turkesterone) display anabolic effects on muscles (Mamatkhanov et al ., ; Syrov et al ., ; Zubeldia et al ., ), even in a sarcopenia context (Lawrence, ). In addition, turkesterone and 20‐hydroxyecdysone have anti‐stress and immunostimulating effects (Shakhmurova et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The minor ecdysteroids from Ajuga turkestanica

Guibout

Mamadalieva

Balducci

et al. 2015

Phytochemical Analysis

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Ajuga turkestanica ecdysteroids are characterised by the abundance of 11α-hydroxylated compounds and by the simultaneous presence of 24C, 27C, 28C and 29C ecdysteroids. It is expected that even more ecdysteroids are to be found in this plant since the starting material for this study lacked the less polar ecdysteroids. The simultaneous presence of 20-hydroxyecdysone and turkesterone (its 11α-hydroxy analogue) as the two major ecdysteroids suggests that every ecdysteroid is probably present in both 11α-hydroxy and 11-deoxy forms.

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Isolation of turkesterone from the epigeal part ofAjuga turkestanica and its anabolic activity

Cited by 13 publications

References 3 publications

In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

Neo-clerodane diterpenes from Ajuga turkestanica

The minor ecdysteroids from Ajuga turkestanica

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