Isolation of type C Virions from a Normal Human Fibroblast Strain

Panem, Sandra; Prochownik, Edward V.; Reale, Frank R.; Kirsten, W. H.

doi:10.1126/science.49927

Cited by 121 publications

(56 citation statements)

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“…The growth and purification of HEL-12 virus from culture fluids of virusproducing cells has been described (Panem et al, 1975). SiSV(SSAV) (Bergholz et al, 1977) produced by marmoset fibroblasts (HF) was grown and purified according to well-established protocols in a different building to avoid any cross-contamination.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Comparative studies have demonstrated that these virus isolates are related to both BaEV and SiSV/GALV Teich et al, 1975;Chan et al, 1976;Panem et al, 1975). One of these isolates, HEL-12 virus, was 0022-1317/81/0000-4399 $02.00 ;~ 1981 SGM spontaneously released from a strain of normal human embryonic lung fibroblasts (Panem et al, 1975). To obtain a more detailed picture of the genetic relationship between exogenous and endogenous primate viruses and HEL-12 virus, RNAs of representative viruses of the SiSV/GALV and BaEV classes and HEL-12 viral RNA were compared by two-dimensional polyacrylamide gel electrophoresis (PAGE) of the large RNase T~-resistant oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Oligonucleotide Analysis of Exogenous and Endogenous Primate Type C Viruses

Hefti

Panem

1981

Journal of General Virology

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SUMMARYRNAs of representative viruses of the exogenous simian sarcoma virus-gibbon ape lymphosarcoma virus (SiSV/GALV) and endogenous baboon virus (BaEV) classes of subhuman primate type C viruses were compared and related to HEL-12 virus, an isolate derived from human embryonic lung cells. The extent of sequence identity between different viral RNA preparations was determined by comparison of fingerprint patterns obtained after electrophoretic separation of RNase Tl-resistant oligonucleotides. The studies presented indicate that HEL-12 viral RNA and simian sarcoma-simian associated virus [SiSV(SSAV)] RNA share 90 to 95 % of the large oligonucleotides. From 5 to 10% of virus-specific oligonucleotides were detected in each of several virus preparations examined and their occurrence was independent of the cell line on which the virus was propagated. HEL-12 virus and GALV-SF have 50% unique oligonucleotides in common. These are the same oligonucleotides that are shared between GALV-SF and SiSV(SSAV) RNA. Two BaEV isolates, M7 and BILN, and RD 114, a BaEV-related endogenous virus of cats, each easily display distinguishable oligonucleotide patterns. Large oligonucleotides characteristic for these three endogenous virus isolates were not detected in the fingerprints of HEL-12 virus, SiSV(SSAV) and GALV-SF.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Oligonucleotide Analysis of Exogenous and Endogenous Primate Type C Viruses

Hefti

Panem

1981

Journal of General Virology

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“…Sci. USA 74 (1977) viruses highly related to mem'bers of this virus group (25)(26)(27)(28)(29)(30) and, more recently, suggestive evidence for antibodies to this virus group in human sera (19,39,40). However, proviral sequences related to the simian sarcoma virus/gibbon ape leukemia virus group have not as yet been reported (14).…”

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confidence: 99%

“…Recently, nucleic acids and proteins related to the two known primate type-C virus groups-the simian sarcoma virus/gibbon ape leukemia virus group and the baboon endogenous virus group-have been reported in fresh human tissues (5-8, 11-14, 16, 18-23) (20)(21)(22)(23) have been contested (24). Although clearly a rare event, isolation of a replicating virus from human tissues related to one or both of these primate type-C viruses has also been reported (25)(26)(27)(28)(29)(30). Until The objective of experiments reported here was to examine DNA from human tissues for sequences related to RNA of MuLVR in more detail and with more carefully prepared cDNA probes.…”

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confidence: 99%

Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hemotopoietic neoplasias and absence in DNA from other tissues.

Aulakh¹,

Gallo²

1977

Proc. Natl. Acad. Sci. U.S.A.

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Nature 262,[190][191][192][193][194][195], because there is no sequence homology between nucleic acids from MuLVR and baboon virus. The absence of these nucleic acid sequences in many tissues of patients with neoplasia and from the few tissues examined from people with nonneoplastic disease suggests that they are not endogenous elements but are acquired after fertilization. Taken together with the previous detection of baboon and woolly monkey type-C viral related components in some human tumors, the results suggest acquisition of at least three types of type-C viral sequences in the human population. Data are accumulating that indicate the presence of type-Ctumor-virus-like components in some human tissues (1-23). Cytoplasmic nucleic acids with sequences related to the RNA of Rauscher murine leukemia virus (MuLVR) and other viruses have been described (4, 9-17). Recently, nucleic acids and proteins related to the two known primate type-C virus groups-the simian sarcoma virus/gibbon ape leukemia virus group and the baboon endogenous virus group-have been reported in fresh human tissues (5-8, 11-14, 16, 18-23) The objective of experiments reported here was to examine DNA from human tissues for sequences related to RNA of MuLVR in more detail and with more carefully prepared cDNA probes. We report: (i) detection of nucleic acid sequences distantly related to MuLVR in DNA from spleens of some patients with acute myelogenous leukemia (AML), Hodgkin's disease, and multiple myeloma; (ii) absence of these sequences in DNA from apparently uninvolved tissues of the same patients with neoplasia and from the normal human tissues tested. Since MuLVR and the baboon endogenous virus are not detectably related (32), the detection of MuLVR-related DNA sequences is not explained by the previously reported presence of proviral sequences of baboon virus in DNA from some leukemia patients. Taken together, the results show at least two independent sets of oncornavirus-related sequences in the DNA from some leukemia patients. While the mode, source, and time of acquisition, and relevance to etiology, must be speculative, interspecies transmission of one or both agents may be involved.MATERIALS AND METHODS Purification of DNA from Human Tissues. The tissues used in these experiments were uncultured specimens obtained at the time of autopsy and kept frozen at -80°until the isolation of DNA. The organ tissues obtained from people with neoplasia were infiltrated with tumor cells. The tissues were lysed in a

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Particles Resembling Oncornaviruses

Bendheim¹

1977

Arch Neurol

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Isolation of type C Virions from a Normal Human Fibroblast Strain

Cited by 121 publications

References 19 publications

Comparative Oligonucleotide Analysis of Exogenous and Endogenous Primate Type C Viruses

Comparative Oligonucleotide Analysis of Exogenous and Endogenous Primate Type C Viruses

Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hemotopoietic neoplasias and absence in DNA from other tissues.

Particles Resembling Oncornaviruses

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