Isolation of Vi Antigen and a Simple Method for Its Measurement

Wong, K. H.; Feeley, John C.

doi:10.1128/aem.24.4.628-633.1972

Cited by 28 publications

(13 citation statements)

References 13 publications

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“…In the present study, the Vi and porin were first purified from S. typhi Ty2 and S. typhi 0901 strains, respectively. The Vi antigen was extracted by a non-denaturing technique adopted by Wong et al (43). This Vi met all of the requirements drafted by W.H.O.…”

Section: Discussionmentioning

confidence: 99%

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Protective Efficacy and Immunogenicity of Vi‐Porin Conjugate against Salmonella typhi

Singh

Ganguly

Kumar

et al. 1999

Microbiology and Immunology

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A conjugate vaccine against Salmonella typhi was prepared by covalently binding capsular polysaccharide (Vi) with porin, both isolated from S. typhi. First, Vi and porins were extracted. The Vi was purified from S. typhi Ty2. The purified Vi conformed to the requirements of the World Health Organization. Porins were purified from S. typhi 0901. The Vi was bound to the porins by a heterobifunctional cross‐linking reagent, N‐succinimidyl‐3‐(2‐pyridyl dithio)‐propionate (SPDP). After preparing the Vi‐porin conjugate, its protective ability and immunogenicity were studied in mice following systemic immunization. The results showed that the conjugate is 6.5‐fold more protective than Vi alone against S. typhi. The mice immunized with conjugate elicited higher anti‐Vi antibody (IgG) levels (P<0.01) than the mice immunized with Vi alone. Anti‐porin antibodies were also induced by the conjugate. To study the mucosal immune responses, secretory IgA (sIgA) in the intestinal fluid was measured. Conjugate‐immunized mice showed the induction of sIgA as compared to Vi alone. The results showed that when Vi is bound to porins, both isolated from same organism, the resultant conjugate induced both systemic and mucosal immune responses and provided better protection against S. typhi than Vi alone.

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Section: Discussionmentioning

confidence: 99%

“…Isolation of Vi antigen. Vi was extracted from acetone killed and dried (AKD) cells of S. typhi Ty2 as previously described (43). The cell extract of AKD cells was treated with deoxyribonuclease, ribonuclease and pronase to remove contaminating nucleic acids and protein.…”

Section: Methodsmentioning

confidence: 99%

Protective Efficacy and Immunogenicity of Vi‐Porin Conjugate against Salmonella typhi

Singh

Ganguly

Kumar

et al. 1999

Microbiology and Immunology

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“…Vi antigen was either received from K. H. Wong at the Center for Disease Control in Atlanta, Ga., or prepared from S. typhosa by the method of Wong and 196 BREEN AND APICELLA Feeley (18). The crude antigen was treated with deoxyribonuclease, ribonuclease, and Pronase and precipitated by the addition of Cetavlon (hexadecylbromethyl ammonium bromide).…”

Section: Methodsmentioning

confidence: 99%

Immunogenicity of gonococcal Gc2 polysaccharide: comparative studies with pneumococcal type III polysaccharide and Salmonella typhosa Vi antigen

Breen¹,

Apicella²

1978

Infect Immun

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A plaque assay technique was used to assess the immunogenicity of a gonococcal cell wall polysaccharide (Gc2 antigen) in BALB/c mice. The Gc2 antigen was shown to be immunogenic, and the kinetics of the response differed from that of a pneumococcal polysaccharide (SSS-III) and a polysaccharide antigen of Salmonella typhosa (Vi antigen). In addition, using antithymocyte sera, the Tlymphocyte dependency of these antigens was investigated. The immune response to the Gc2 antigen was demonstrated to be dependent on a population of helper T cells, whereas the response to SSS-III appears to be regulated by suppressor T cells. There appears to be marked differences in the immune response of mice to different bacterial polysaccharides.

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“…of radioactivity were added to triplicate 0-05 ml aliquots of a Vi (or O) antigsn solution (1 mg/ml) in tubes. The Vi antigen was prepared from Citrobacter 5396/38 by the ethanol-cetavlon mild precipitation method (Wong and Feeley, 1972) while ths O antigen was prepared from a Salmonella typhi O strain NCTC 8753 by the hot phenol-water extraction method (Westphal, Liideritz and Bister, 1952). After incubating at 37° for 1 h. an optimal amount of rabbit Vi or O antiserum, pre-determined by adding increasing volumes of the antisera to their corresponding antigen solutions, was added to bring about maximum precipitation.…”

Section: E^timation Of the Agglutinating Capacity Of The Igg And Igmmentioning

confidence: 99%

DIFFERENTIAL AGGLUTINATION OF PARTICULATE Vi AND O ANTIGENS BY THE IgM AND IgG CLASS ANTIBODIES

Chau

1978

Aust J Exp Biol Med

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Isolation of Vi Antigen and a Simple Method for Its Measurement

Cited by 28 publications

References 13 publications

Protective Efficacy and Immunogenicity of Vi‐Porin Conjugate against Salmonella typhi

Protective Efficacy and Immunogenicity of Vi‐Porin Conjugate against Salmonella typhi

Immunogenicity of gonococcal Gc2 polysaccharide: comparative studies with pneumococcal type III polysaccharide and Salmonella typhosa Vi antigen

DIFFERENTIAL AGGLUTINATION OF PARTICULATE Vi AND O ANTIGENS BY THE IgM AND IgG CLASS ANTIBODIES

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