Isolation of viruses from specimens submitted for direct immunofluorescence test for respiratory syncytial virus

Waner, Joseph L.; Whitehurst, Norma J.; Jonas, Steven; Wall, Lester; Shalaby, Hamed

doi:10.1016/s0022-3476(86)80993-3

Cited by 26 publications

(18 citation statements)

References 9 publications

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“…We found that 9.5% of our FA-positive and culturepositive (TP) specimens for RSV yielded other viral agents on culture. Similar observations have been reported by Waner et al (8). We agree with their suggestion that selective culturing or inclusion of a broader routine battery of…”

Section: Discussionsupporting

confidence: 94%

Efficiency of immunofluorescence for rapid detection of common respiratory viruses

Ray¹,

Minnich²

1987

J Clin Microbiol

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Rapid immunofluorescence (FA) methods for the detection of common respiratory viruses were compared with culture results over a 3-year period to assess the relative efficiency of FA in a clinical laboratory setting. For respiratory syncytial virus, efficiencies were high (sensitivity, 90 to 95%; specificity, 92 to 95%). The sensitivity of FA for detection of parainfluenza virus type 1, parainfluenza virus type 3, influenza A virus, and adenoviruses ranged from 28 to 63%, but specificities for these viruses were uniformly 98 to 100%. The observations form a basis for consideration of selective reduction of routine culture procedures for specimens with initial positive rapid FA results; however, the possibility of dual viral infection in some situations must also be considered.

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Section: Discussionsupporting

confidence: 94%

Efficiency of immunofluorescence for rapid detection of common respiratory viruses

Ray¹,

Minnich²

1987

J Clin Microbiol

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“…When considering the cohorting of inpatients, it is important to realize that the aforementioned infectious agents may cause bronchiolitis in isolation or in combination with other infectious agents. Although older studies report coinfections (eg, detection of two or more viruses from the same biologic sample) in 4.4% to 23.7% of children younger than 3 years of age who had respiratory illnesses, [69][70][71][72][73][74] recent studies have found 20% to 27% coinfections when testing hospitalized children who had LRTI. 59,61 There is a lack of clear data, however, about the clinical characteristics of children who have coinfections.…”

Section: Coinfectionsmentioning

confidence: 99%

Respiratory Viruses in Bronchiolitis and Their Link to Recurrent Wheezing and Asthma

Mansbach

Camargo

2009

Clinics in Laboratory Medicine

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Bronchiolitis is the leading cause of hospitalization for children age <1 year and these hospitalized children have an increased risk of developing childhood asthma. It remains unclear, however, which children with severe bronchiolitis (e.g., an episode requiring hospitalization) will develop recurrent wheezing and/or asthma. Although many environmental and genetic factors may play a role in the pathway from bronchiolitis to asthma, this review focuses on the viruses that have been linked to bronchiolitis and how these viruses may predict or contribute to future wheezing and asthma. The review also discusses vitamin D as an emerging risk factor for respiratory infections and wheezing.

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“…DIF permits rapid diagnosis of multiple viruses in the same specimen. Cells obtained from a nasopharyngeal wash or aspirate, bronchial lavage, or lesion may be apportioned to numerous circumscribed wells on glass slides and stained by IF for as many viruses as the availability of cells and reagents permits (81,106). The viruses present are thereby identified in their natural host cell(s).…”

Section: Diagnostic Strategiesmentioning

confidence: 99%

Mixed viral infections: detection and management

Waner

1994

Clin Microbiol Rev

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An analysis was done of the incidence and nature of mixed virus infections diagnosed in the same clinical specimen from immunocompetent patients; respiratory viruses were emphasized. Few studies have addressed mixed viral infections in any systematic fashion. The relevant studies reviewed focused on clinical relationships or diagnostic methods. Data relating to multiple infections were usually derived incidentally to the purpose of the investigations. Sixty-three percent of the reports with data on mixed infections identified them in < 5% of the total number of viral infections. Respiratory syncytial virus was the most common coinfecting virus, and respiratory syncytial virus and influenza virus were the most common virus pair identified. In considering rapid diagnostic techniques, in 87% of the reports with available data a virus was diagnosed in > 10% of specimens that were negative for the virus targeted by one method. There was no indication that mixed infections were associated with increased disease in immunocompetent patients or in certain immunocompromised patients. Immunocompromised patients, however, appeared to have a greater incidence of multiple infections. Mixed infections of single cells also occur and may have important clinical implications relative to reactivation of latent viruses and enhanced disease. The requirement for a comprehensive strategy for viral diagnosis involving multiple techniques was indicated by these findings.

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Isolation of viruses from specimens submitted for direct immunofluorescence test for respiratory syncytial virus

Cited by 26 publications

References 9 publications

Efficiency of immunofluorescence for rapid detection of common respiratory viruses

Efficiency of immunofluorescence for rapid detection of common respiratory viruses

Respiratory Viruses in Bronchiolitis and Their Link to Recurrent Wheezing and Asthma

Mixed viral infections: detection and management

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