Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Poret, Jonquil M.; Molina, Patricia E.; Simon, Liz

doi:10.3791/61732-v

Cited by 3 publications

(2 citation statements)

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“…Therefore, this protocol provides a powerful tool to manipulate protein and non-coding RNA expression and study their role on adipocyte functions. To get closer to ideal physiological conditions, Poret et al describe a method to generate functional mature adipocytes by using adipose-derived stem cells (ADSCs) obtained from rhesus macaque AT 7 . This protocol explains how to isolate primary ADSCs and how to induce their proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Exploring Adipose Tissue Functions

Jager

Gilleron

2023

JoVE

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Section: Discussionmentioning

confidence: 99%

Exploring Adipose Tissue Functions

Jager

Gilleron

2023

JoVE

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“…The stromal vascular fraction (SVF), containing adipose stem cells/preadipocytes, was isolated from iWAT or eWAT as previously described (Aune et al , 2013; Poret et al , 2021). Briefly, iWAT or eWAT of 8–10 weeks C57BL/6J male mice was collected quickly and chopped into small chunks in ice‐cold PBS.…”

Section: Methodsmentioning

confidence: 99%

Role and mechanism of PVN–sympathetic–adipose circuit in depression and insulin resistance induced by chronic stress

Wang,

Sun,

You

et al. 2023

EMBO Reports

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Chronic stress induces depression and insulin resistance, between which there is a bidirectional relationship. However, the mechanisms underlying this comorbidity remain unclear. White adipose tissue (WAT), innervated by sympathetic nerves, serves as a central node in the interorgan crosstalk through adipokines. Abnormal secretion of adipokines is involved in mood disorders and metabolic morbidities. We describe here a brain–sympathetic nerve–adipose circuit originating in the hypothalamic paraventricular nucleus (PVN) with a role in depression and insulin resistance induced by chronic stress. PVN neurons are labelled after inoculation of pseudorabies virus (PRV) into WAT and are activated under restraint stress. Chemogenetic manipulations suggest a role for the PVN in depression and insulin resistance. Chronic stress increases the sympathetic innervation of WAT and downregulates several antidepressant and insulin‐sensitizing adipokines, including leptin, adiponectin, Angptl4 and Sfrp5. Chronic activation of the PVN has similar effects. β‐adrenergic receptors translate sympathetic tone into an adipose response, inducing downregulation of those adipokines and depressive‐like behaviours and insulin resistance. We finally show that AP‐1 has a role in the regulation of adipokine expression under chronic stress.

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