Isolation, Purification, and Characterization of a Collagen-associated Serpin, Caspin, Produced by Murine Colon Adenocarcinoma Cells

Kozaki, Ken Ichi; Miyaishi, Osamu; Koiwai, Osamu; Yasui, Yoshihiro; Kashiwai, Akiko; Nishikawa, Yohko; Shimizu, Satoru; Saga, Shinsuke

doi:10.1074/jbc.273.24.15125

Cited by 56 publications

(47 citation statements)

References 25 publications

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“…Caspin, a murine homologue to pigment epithelium-derived factor, which binds specifically to collagen I and III in the extracellular matrix, is highly expressed in nonmetastatic colon cancer cell lines and low in metastatic variants demonstrating a relation among pigment epithelium-derived factor expression, metastasis, and cell adhesion (44). In line with this, overexpression of pigment epitheliumderived factor decreases the invasiveness of glioma cells in vitro (45).…”

Section: Discussionsupporting

confidence: 49%

Decreased Pigment Epithelium-Derived Factor Is Associated with Metastatic Phenotype in Human and Rat Prostate Tumors

Halin¹,

Wikström²,

Rudolfsson

et al. 2004

Cancer Research

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Pigment epithelium-derived factor, a potent angiogenesis inhibitor in the eye, is also expressed in the prostate. Prostate size and angiogenesis is increased in pigment epithelium-derived factor knockout mice, and pigment epithelium-derived factor is down-regulated in some prostate cancers. To investigate whether pigment epithelium-derived factor expression correlates with tumor progression, we examined 5 Dunning rat prostate sublines with different growth rates, differentiation, androgen dependence, vascular density, and metastatic ability and 26 human prostate cancers of Gleason score 8 -10 obtained from patients at transurethral resection selected to represent two groups, with and without metastases at diagnosis. By Western blot, real-time quantitative reverse transcription-PCR, and immunostaining, pigment epithelium-derived factor was detected in highly differentiated, nonmetastatic, androgen-sensitive Dunning tumors and in the anaplastic, androgen insensitive but nonmetastatic Dunning tumors. In contrast, the metastatic Dunning tumor sublines showed very low pigment epithelium-derived factor expression levels. In human cancer tissues, by immunohistochemistry and real-time quantitative reverse transcription-PCR, patients without metastases at diagnosis had higher tumor pigment epithelium-derived factor levels than tumors from patients with metastases at diagnosis. In both the rat model and in the human tumors, the proliferation index and vascular count, as determined by Ki-67 staining and endoglin and/or factor VIII-related antigen staining, inversely correlated with pigment epithelium-derived factor mRNA levels. These observations indicate that loss of pigment epitheliumderived factor expression could be associated with the progression toward a metastatic phenotype in prostate cancer.

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Section: Discussionsupporting

confidence: 49%

Decreased Pigment Epithelium-Derived Factor Is Associated with Metastatic Phenotype in Human and Rat Prostate Tumors

Halin¹,

Wikström²,

Rudolfsson

et al. 2004

Cancer Research

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“…In contrast, in cases of lesions showing PEDF deposition in acellular area associated with frequent apoptotic neovessels, deposited PEDF may exert its antiangiogenic activity. This hypothesis is reasonable, because PEDF intimately associates with the extracellular matrix 26 and forms a complex with collagen type I. 27 A limitation related to the current study involves only correlative observations between localization of PEDF and density of microvessels and apoptosis in human coronary arteries, without direct evidence of antiangiogenic properties of PEDF, which has been uncertain.…”

Section: Discussionmentioning

confidence: 98%

Cytoplasmic Expression and Extracellular Deposition of an Antiangiogenic Factor, Pigment Epithelium-Derived Factor, in Human Atherosclerotic Plaques

Baba

Yonemitsu

Nakano

et al. 2005

ATVB

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Objective-To assess the expression and distribution of a neurotrophic/antiangiogenic factor, pigment epithelium-derived factor (PEDF), related to angiogenesis that is a possibly key event during atherogenesis in human atherosclerotic plaques. Methods and Results-Twenty fresh aortic samples were used for reverse-transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry (IHC). In addition, 80 stocked paraffin blocks of coronary arteries from 40 autopsy cases were also used. IHC revealed divergent staining patterns for PEDF in both the aortas and the coronary arteries tested, ie, "cytoplasmic staining" or "extracellular deposition," were observed, respectively. In the areas showing cytoplasmic staining, double PEDF was expressed in a majority of the foamy macrophages and in some smooth muscle cells, and the PEDF-positive cell frequency was positively correlated with that of microvessels in a cell-rich area in the coronary arteries (PϽ0.0001). Inversely, extracellular deposition of PEDF was seen in acellular areas and was negatively correlated with the number of microvessels (Pϭ0.0003). Conclusions-These

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“…All immunostained specimens were examined under a confocal laser scanning microscope (LSM 410; Karl Zeiss, Oberkochen, Germany). Sections of mouse embryonic tissues were prepared, and the immunohistochemical staining of these sections was performed as described previously (28).…”

Section: Methodsmentioning

confidence: 99%

Phosphorylation of αB-crystallin in Mitotic Cells and Identification of Enzymatic Activities Responsible for Phosphorylation

Kato¹,

Ito²,

Kamei³

et al. 1998

Journal of Biological Chemistry

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121

115

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The immunofluorescence localization of ␣B-crystallin in U373 MG human glioma cells with an antibody specific for ␣B-crystallin that had been phosphorylated at Ser-45 revealed an intense staining of cells in the mitotic phase of the cell cycle. Phosphorylated forms of ␣B-crystallin in mitotic cells were detected in all cell lines examined and in tissue sections of mouse embryos. Increases in the levels of ␣B-crystallin that had been phosphorylated at Ser-45 and Ser-19, but not at Ser-59, were detected biochemically by isoelectric focusing or SDSpolyacrylamide gel electrophoresis and a subsequent Western blot analysis of extracts of cells collected at the mitotic phase. When we estimated the phosphorylation activity specific for ␣B-crystallin in extracts of mitotic U373 MG cells, using the amino-terminal 72-amino acid peptide derived from unphosphorylated ␣B2-crystallin as the substrate, we found that the activities responsible for the phosphorylation of Ser-45 and Ser-19 were markedly enhanced but that the activity responsible for the phosphorylation of Ser-59 was suppressed ␣-Crystallin, a major structural protein of the vertebrate eye lens, is a polymeric protein with a molecular mass of about 800 kDa. The ␣-crystallin of the bovine lens is composed predominantly of two types of polypeptide, the A (␣A1 and ␣A2) and B (␣B1 and ␣B2) subunits (1). ␣A1 and ␣B1 are the phosphorylated forms of the primary gene products, ␣A2 and ␣B2 (2, 3). The molecular mass of each subunit is about 20 kDa, and the similarity between the primary structures of the two subunits is greater than 50% (4). The ␣-crystallins also share sequence similarity with the small heat shock proteins (hsps) 1 of numerous species (5). Because of the striking similarities among the primary structures of the carboxyl-terminal half of each molecule (the ␣-crystallin domain), ␣A-crystallin, ␣B-crystallin, hsp27, and p20 (6) are considered to be members of the ␣-crystallin small hsp family in vertebrates (7,8).A common feature of small hsps is their formation of large oligomeric complexes such as ␣A-crystallin and ␣B-crystallin in the lens. In the skeletal muscle, ␣B-crystallin, hsp27, and p20 seem to form a large heteropolymer, because the three proteins were copurified from the extract and coimmunoprecipitated with antibodies against each of the three proteins (6, 9). The ␣-crystallin domain of each small hsp was suggested to be important for this complex formation and the chaperone activity (8). Both ␣A-crystallin (10) and ␣B-crystallin (11) are also present in nonlenticular tissues, and the expression of ␣B-crystallin, but not ␣A-crystallin and p20, is induced in cells under various stressful conditions, as is hsp27 (12, 13).The major posttranscriptional modifications of the ␣-crystallin small hsp family are due to the phosphorylation of serine residues. The phosphorylation of hsp27 by mitogen-activated protein (MAP) kinase-activated protein (MAPKAP) kinase-2 is enhanced when cells are exposed to heat (14, 15) or chemicals (16). p20 in vascular smooth m...

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Isolation, Purification, and Characterization of a Collagen-associated Serpin, Caspin, Produced by Murine Colon Adenocarcinoma Cells

Cited by 56 publications

References 25 publications

Decreased Pigment Epithelium-Derived Factor Is Associated with Metastatic Phenotype in Human and Rat Prostate Tumors

Decreased Pigment Epithelium-Derived Factor Is Associated with Metastatic Phenotype in Human and Rat Prostate Tumors

Cytoplasmic Expression and Extracellular Deposition of an Antiangiogenic Factor, Pigment Epithelium-Derived Factor, in Human Atherosclerotic Plaques

Phosphorylation of αB-crystallin in Mitotic Cells and Identification of Enzymatic Activities Responsible for Phosphorylation

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