Isolation, purification and characterization of Trichothecinol-A produced by endophytic fungus Trichotheciumsp. and its antifungal, anticancer and antimetastatic activities

Taware, Ravindra; Abnave, Prasad; Patil, Deepak; Rajamohananan, Pattuparambil Ramanpillai; Raja, Remya; Soundararajan, Gowrishankar; Kundu, Gopal C.; Ahmad, Absar

doi:10.1186/2043-7129-2-8

Cited by 21 publications

(2 citation statements)

References 21 publications

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“…an endophytic fungus isolated from Phyllanthus amarus collected from Pune India. Compound 101 showed activity against Cryptococcus albidus (NCIM 3372) up to 20 µg/mL [ 87 ]. Trichothecin (169) ( Figure 8 ), a sesquiterpene, was isolated from endophytic fungus Trichothecium sp.…”

Section: Medicinal Plantsmentioning

confidence: 99%

Endophytic Fungi: A Source of Potential Antifungal Compounds

Deshmukh

Gupta

Prakash

et al. 2018

JoF

108

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The emerging and reemerging forms of fungal infections encountered in the course of allogeneic bone marrow transplantations, cancer therapy, and organ transplants have necessitated the discovery of antifungal compounds with enhanced efficacy and better compatibility. A very limited number of antifungal compounds are in practice against the various forms of topical and systemic fungal infections. The trends of new antifungals being introduced into the market have remained insignificant while resistance towards the introduced drug has apparently increased, specifically in patients undergoing long-term treatment. Considering the immense potential of natural microbial products for the isolation and screening of novel antibiotics for different pharmaceutical applications as an alternative source has remained largely unexplored. Endophytes are one such microbial community that resides inside all plants without showing any symptoms with the promise of producing diverse bioactive molecules and novel metabolites which have application in medicine, agriculture, and industrial set ups. This review substantially covers the antifungal compounds, including volatile organic compounds, isolated from fungal endophytes of medicinal plants during 2013–2018. Some of the methods for the activation of silent biosynthetic genes are also covered. As such, the compounds described here possess diverse configurations which can be a step towards the development of new antifungal agents directly or precursor molecules after the required modification.

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Section: Medicinal Plantsmentioning

confidence: 99%

Endophytic Fungi: A Source of Potential Antifungal Compounds

Deshmukh

Gupta

Prakash

et al. 2018

JoF

108

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“…Based on its characterization, SJC2 was identified as Trichothecium sp. Several Trichothecium sp species produce trichothecinol-A, which has antifungal, anticancer, and antimetastatic effects (Taware et al, 2014). The compounds 3hydroxyartemisinic acid, 3,15-dihydroxyartemisinic acid, and 3-Oxoartemisinic acid produced by the biotransformation of T. roseum's artemisinic acid have antifungal properties as well (Singh et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

Aini

Elfita

Widjajanti

et al. 2023

molekul

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Endophytic fungi isolated from medicinal plants have high diversity and the secondary metabolites produced have biological activity. Endophytic fungi isolated from medicinal plants have been the subject of many studies on their diversity and biological activity, one of which is jambu mawar (Syzygium jambos (L.) Alston). S. jambos has long been used as a traditional medicine to treat infections caused by pathogenic bacteria in many parts of the world, including South Sumatra. This study purposed to explore the diversity of endophytic fungi isolated from the branches of S. jambos, the antibacterial activity of endophytic fungi extracts, the determination of the structure of the compounds, and the activity of the active compounds of the selected endophytic fungi. Observations of the morphological characteristics of endophytic fungi were macroscopically and microscopically. The endophytic fungal extracts were then tested for antimicrobial activity against test bacteria Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Escherichia coli using the Kirby-Bauer paper disk diffusion method. Isolation of pure compounds using a gravity chromatography column, the determination of the structure of the compound is determined based on 1H-NMR, 13C-NMR, HMQC, and HMBC spectroscopy. Four endophytic fungi, code SJC1–4, were isolated from a branch of S. jambos. The results of macroscopic and microscopic morphological characterization showed three genera of Botryosphaeria, Trichothecium, and Aspergillus. The endophytic fungal isolate SJC1 exhibited strong activity against Gram-positive bacteria and moderate activity against Gram-negative bacteria, while SJC2–4 showed moderate activity against the four bacteria. Molecular identification of SJC1 revealed that it was Botryosphaeria mamane. Isolation of SJC1’s pure compound yielded compound 1, which was identified as 5-acetyl-6-hydroxy-3-methyl-2H-pyran-2-one. This compound is thought to have antibacterial properties.

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An Update on Bioactive Natural Products from Endophytic Fungi of Medicinal Plants

Sharma

Abrol

et al. 2019

Environmental Chemistry for a Sustainable World

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Isolation, purification and characterization of Trichothecinol-A produced by endophytic fungus Trichotheciumsp. and its antifungal, anticancer and antimetastatic activities

Cited by 21 publications

References 21 publications

Endophytic Fungi: A Source of Potential Antifungal Compounds

Endophytic Fungi: A Source of Potential Antifungal Compounds

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

An Update on Bioactive Natural Products from Endophytic Fungi of Medicinal Plants

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