Two new ring A-cleaved lanostane-type triterpenoids, glometenoid A (1) and glometenoid B (2), together with four known steroids, (20S,22E,24R)-ergosta-7,22-dien-3b,5a,6b-triol (3), (3b,5a,8a,22E)-ergosta-6,22-diene-3,5,8-triol (4), 5a,8aepidioxy-22E-ergosta-6,22-dien-3b-ol (5), and ergosterol (6), were isolated from the endophytic fungus Glomerella sp. F00244. Their structures were elucidated by comprehensive spectroscopic analyses of NMR and MS data. Their antimicrobial activities were evaluated against pathogenic bacteria Bacillus subtilis ATCC 9372, Staphylococcus aureus ATCC 25923, Bacillus pumilus CMCC (B) 63202, Micrococcus luteus CMCC28001, and pathogenic fungi Candida albicans AS2.538 and Aspergillus niger ACCC30005, but no inhibition was observed at a concentration of 20 lg/ml. Further cytotoxicity assessment revealed that compound 1 exhibited weak antiproliferative activity against ovarian cancer HeLa cell.