Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R.Br.)

Alam, Mohammad; Auddy, Biswajit; Gomes, Antony

doi:10.1016/0041-0101(94)90314-x

Cited by 75 publications

(47 citation statements)

References 3 publications

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“…In the present study, the protection of the mesenteric cells against the degranulating effect of venom by the ethanol leaf extract may be by its interaction with phospholipase A2 and by prevention of the release of platelet activating factor and histamine from mast cells. It has been reported that anti oxidant activity is one of the mechanisms of venom inactivation and inhibition (Alam et al, 1994;Gomes, 1998, 2003). In our study, blood TBARS levels were significantly reduced and non-protein sulfhydral (GSH) and catalase levels in kidney were significantly enhanced by the extract.…”

Section: Discussionmentioning

confidence: 98%

Neutralization potential of Viper russelli russelli (Russell’s viper) venom by ethanol leaf extract of Acalypha indica

Shirwaikar

Rajendran

Bodla

et al. 2004

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 98%

Neutralization potential of Viper russelli russelli (Russell’s viper) venom by ethanol leaf extract of Acalypha indica

Shirwaikar

Rajendran

Bodla

et al. 2004

Journal of Ethnopharmacology

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“…Its extract mainly consists of essential oils and phytosterols, like hemidesmol, hemidesterol and saponins. The active principle of H. indicus is 2-hydroxy 4-methoxy benzoic acid (HMBA) [12]. It is also known to have antioxidant properties [13].…”

Section: Introductionmentioning

confidence: 99%

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Saravanan

Nalini

2008

Cellular and Molecular Biology Letters

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Alcoholic liver disease (ALD) is one of the most common diseases in modern society. A large number of studies are in progress aiming to identify natural substances that would be effective in reducing the severity of ALD. Although there are currently a number of drugs on the market, their long-term use can have numerous side effects. Hemidesmus indicus is an indigenous Ayurvedic medicinal plant used in soft drinks in India. In this study, we examined the effects of its ethanolic root extract on experimental liver damage in order to evaluate its hepatoprotective effects against hepatotoxicity induced in rats by ethanol at a dosage of 5 g/kg body weight for 60 days. The H. indicus root extract was given at a dose of 500 mg/kg body weight for the last 30 days of the experiment. The animals were monitored for food intake and weight gain. The liver was analysed for the degree of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) and antioxidant status using the activities of glutathione-depedendant enzymes. The degree of liver damage was analysed using serum marker enzyme activities, the total protein, albumin, globulin, ceruloplasmin and liver glycogen contents, and the A/G ratio. The Fourier transform infrared spectra (FT-IR) of the liver tissues were recorded in the region of 4000-400 cm -1 . The ethanol-fed rats showed significantly elevated liver marker enzyme activities, lipid peroxidation levels and reduced antioxidant levels as compared to the control rats. Oral administration of H. indicus for the latter 30 days resulted in an increased food intake and weight gain, decreased TBARS levels, near normal levels of glutathione-dependent enzymes, increased total protein, albumin, globulin and liver glycogen contents, an increased A/G ratio, and decreased liver marker enzyme activities and ceruloplasmin levels. The relative intensity of the liver FT-IR bands for the experimental groups were found to be altered significantly (p < 0.05) compared to the control samples. For the group that had H. indicus co-administered with ethanol, the intensity of the bands was near normal. Moreover, the results of the FT-IR study correlated with our biochemical results.

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“…Some of them have been characterized as inhibitors of cyclooxygenase and lipoxygenase activities (24). Additionally, some plants active against the lethal effect of snake venoms (25,26, present study) are also active against the edema induced by venoms. The herbal compound (2-OH-4-methoxy benzoic acid) isolated from the root extract of Hemidesmus indicus R. BR effectively neutralized the inflammation induced by Vipera russelli venom in male albino mice and reduced the cotton pelletinduced granuloma in rats (27).…”

Section: Discussionmentioning

confidence: 98%

Neutralization of the edema-forming, defibrinating and coagulant effects of Bothrops asper venom by extracts of plants used by healers in Colombia

Núñez

Otero

Barona

et al. 2004

Braz J Med Biol Res

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We determined the neutralizing activity of 12 ethanolic extracts of plants against the edema-forming, defibrinating and coagulant effects of Bothrops asper venom in Swiss Webster mice. The material used consisted of the leaves and branches of Bixa orellana (Bixaceae), Ficus nymphaeifolia (Moraceae), Struthanthus orbicularis (Loranthaceae) and Gonzalagunia panamensis (Rubiaceae); the stem barks of Brownea rosademonte (Caesalpiniaceae) and Tabebuia rosea (Bignoniaceae); the whole plant of Pleopeltis percussa (Polypodiaceae) and Trichomanes elegans (Hymenophyllaceae); rhizomes of Renealmia alpinia (Zingiberaceae), Heliconia curtispatha (Heliconiaceae) and Dracontium croatii (Araceae), and the ripe fruit of Citrus limon (Rutaceae). After preincubation of varying amounts of each extract with either 1.0 µg venom for the edema-forming effect or 2.0 µg venom for the defibrinating effect, the mixture was injected subcutaneously (sc) into the right foot pad or intravenously into the tail, respectively, to groups of four mice (18-20 g). All extracts (6.2-200 µg/mouse) partially neutralized the edema-forming activity of venom in a dose-dependent manner (58-76% inhibition), with B. orellana, S. orbicularis, G. panamensis, B. rosademonte, and D. croatii showing the highest effect. Ten extracts (3.9-2000 µg/mouse) also showed 100% neutralizing ability against the defibrinating effect of venom, and nine prolonged the coagulation time induced by the venom. When the extracts were administered either before or after venom injection, the neutralization of the edema-forming effect was lower than 40% for all extracts, and none of them neutralized the defibrinating effect of venom. When they were administered in situ (sc at the same site 5 min after venom injection), the neutralization of edema increased for six extracts, reaching levels up to 64% for C. limon.

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Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R.Br.)

Cited by 75 publications

References 3 publications

Neutralization potential of Viper russelli russelli (Russell’s viper) venom by ethanol leaf extract of Acalypha indica

Neutralization potential of Viper russelli russelli (Russell’s viper) venom by ethanol leaf extract of Acalypha indica

Hemidesmus indicus Protects against ethanol-induced liver toxicity

Neutralization of the edema-forming, defibrinating and coagulant effects of Bothrops asper venom by extracts of plants used by healers in Colombia

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