Isolation, screening and molecular characterization of phytase-producing microorganisms to discover the novel phytase

Nezhad, Nima Ghahremani; Rahman, Raja Noor Zaliha Raja Abd; Normi, Yahaya M.; Oslan, Siti Nurbaya; Shariff, Fairolniza Mohd; Leow, Adam Thean Chor

doi:10.1007/s11756-023-01391-w

Cited by 8 publications

(3 citation statements)

References 45 publications

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“…In earlier studies, phytase enzyme-producing bacterial isolates were screened and identified from various sources, including poultry farms, dairy areas, hydrothermal vents, hot springs, agricultural soil, etc. [7,[13][14][15][16][17][18][19]. In the present study, screening of phytase-producing bacterial isolates obtained from soil samples in the north Gujarat region was conducted.…”

Section: Resultsmentioning

confidence: 99%

Screening and molecular characterization of phytase producer from north Gujarat region

Madhvi,

Shah

2024

J App Biol Biotech

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Soil samples from the rhizosphere region of different agricultural fields, cattle dung sheds, dump sites of agricultural and dairy waste, etc. in the north Gujarat area were used for the screening of phytase-producing bacteria. Out of the 104 microbial colonies, around 42 colonies showed phytase production on Petri plates, as indicated by the zone of hydrolysis around the colonies. Among them, 13 isolates with hydrolysis efficiency more than 100% were screened for quantitative enzyme production in submerged fermentation. Two isolates, designated as which was further subjected to study the effect of physiological parameters including temperature, pH, incubation time, and inoculum percent. The effect of different physiological parameters on the production of enzymes suggests that each isolate has its own unique set of conditions for higher enzyme production. Isolate DSS was found to have higher enzyme production at 40°C with 5% inoculum and CDS at 55°C with 10% inoculum; both isolates gave higher enzyme production at pH 6 and 72 h of incubation time under submerged fermentation conditions. Based on the molecular identification and identity score,

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Section: Resultsmentioning

confidence: 99%

Screening and molecular characterization of phytase producer from north Gujarat region

Madhvi,

Shah

2024

J App Biol Biotech

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“…Species-level identification of the chosen isolates comes next, following the screening and selection of the best phytase-producing microbes. 16S rRNA gene sequencing is typically used for the molecular identification of bacteria in general and phytase-producing bacteria in particular [ 18 , 38 ]. In one study, 16S rRNA gene sequencing was used to identify the chosen phytase-producing bacterial isolates after microscopic inspection [ 12 ].…”

Section: Identification Of Phytase-producing Microbementioning

confidence: 99%

Review on Desirable Microbial Phytases as a Poultry Feed Additive: Their Sources, Production, Enzymatic Evaluation, Market Size, and Regulation

Urgessa,

Koyamo,

Dinka

et al. 2024

International Journal of Microbiology

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Poultry’s digestive tract lacks hydrolytic phytase enzymes, which results in chelation of dietary minerals, vital amino acids, proteins, and carbohydrates, phytate‐phosphate unavailability, and contamination of the environment due to phosphorus. Therefore, it is necessary to use exogenous microbial phytases as feed additive to chicken feed to catalyze the hydrolysis of dietary phytate. Potential sources of microbial isolates that produce desired phytases for chicken feed supplementation have been isolated from agricultural croplands. It is achievable to isolate phytase‐producing bacteria isolates using both broth and agar phytase screening media. Potential substrates for submerged fermentation (SmF) for bacterial phytase production and solid‐state fermentation (SSF) for fungal phytase production include rice and wheat bran. Following fermentation, saturated ammonium sulphate precipitation is typically used to partially purify microbial culture filtrate. The precipitate is then desalted. Measurements of the pH optimum and stability, temperature optimum and stability, metal ions stability, specificity and affinity to target substrate, proteolysis resistance, storage stability, and in vitro feed dephosphorylation are used to perform an enzymatic evaluation of phytase as an additive for poultry feed. The growth of the feed phytase market is primarily due to the expansion of chicken farms to meet the demand for meat and eggs from humans. The Food and Drug Administration in the USA and the European Food and Safety Authority are primarily in charge of putting rules pertaining to feed phytase use in chicken feed into effect. Conclusively, important components of the production of phytase additives for poultry feed include identifying a reliable source for potential microbe isolation, selecting an economical method of phytase production, thoroughly characterizing the biochemical properties of phytase, and comprehending the size and regulation of the current feed phytase market.

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“…and Klebsiella variicola . Similarly, Nezhad et al (2023) isolated eleven phytase-producing microorganisms from a more diverse range of sources, including the bottom of a lake, cow stool, compost, decaying corn fruit, soil, and pineapple. Sequencing analysis of 16S rDNA and nuclear ribosomal transcribed spacer (ITS) genes revealed that six of the eleven isolates belonged to the Acinetobacter genus, with two isolates affiliated with the Enterobacter genus.…”

Section: Introductionmentioning

confidence: 99%

Identification of phytase producing bacteria from acidifying Tithonia diversifolia: Potential for ruminant feed development

Pazla,

Yanti,

Jamarun

et al. 2024

Saudi Journal of Biological Sciences

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Isolation, screening and molecular characterization of phytase-producing microorganisms to discover the novel phytase

Cited by 8 publications

References 45 publications

Screening and molecular characterization of phytase producer from north Gujarat region

Screening and molecular characterization of phytase producer from north Gujarat region

Review on Desirable Microbial Phytases as a Poultry Feed Additive: Their Sources, Production, Enzymatic Evaluation, Market Size, and Regulation

Identification of phytase producing bacteria from acidifying Tithonia diversifolia: Potential for ruminant feed development

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