Isolation, Screening and Optimization for Laccase production by  Scytalidium lignicola pesante under submerged fermentation

Darade, Amanpreet K. Sidhu Sonali B.; Gaikwad, Praneeta P. Bhavsar Vishwas B.; Patil, Sucheta N.

doi:10.20546/ijcmas.2017.604.289

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…The anti-MRSA activity declined slightly with further increment in inoculum sizes. Similar observation also reported by Sidhu et al, (2017) in which two mycelial discs was sufficient to stimulate higher laccase production by Scytalidium lignicola under submerged fermentation. Higher inoculum sizes than the optimal may stimulate too much production of microbial biomass which lead to nutrient depletion for their secondary metabolite production (Kumar et al, 2010).…”

Section: Discussionsupporting

confidence: 90%

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Taufiq¹,

Darah²

2019

J Pure Appl Microbiol

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Citation: M.M.J. Taufiq and I. Darah, Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System, J Pure Appl Microbiol., 2019; 13(4):Abstract The effects of cultural conditions on the production of anti-MRSA activity of the endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 isolated from the leaf of a local medicinal herb, Ocimum sanctum Linn. was studied. The highest anti-MRSA activity of 50.0±0.1 U/mL and 1.46±0.1 g/L of fungal growth were obtained after incorporating all the improved cultural conditions which consisted of yeast extract sucrose broth supplemented with host plant extract, with initial medium pH of 6, 2 mycelial plugs of 4 days old seed culture, cultivation temperature of 30°C and cultivated for 16 days under static conditions without the presence of light. After improvement of cultural conditions, a significant increment of anti-MRSA activity of about 57.57% (2.4 folds) and a slight increment of fungal growth of about 8. 15% (1.1 folds) were obtained compared to the cultural condition before improvement. Indeed the improvement of cultural conditions greatly affected the anti-MRSA activity and growth production by L. pseudotheobromae IBRL OS-64 isolate.

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Section: Discussionsupporting

confidence: 90%

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Taufiq¹,

Darah²

2019

J Pure Appl Microbiol

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“…In previous studies, similarly, Scytalidium lignicola pesante (Sidhu et al 2017) and Myrothecium verrucaria (Sun et al 2017), P. eryngii, P. florida and P. sajor caju (Mathur et al 2021), Beauveria pseudobassiana (Sharma et al 2023), Beltraniopsis sp. ET-17and…”

Section: Discussionmentioning

confidence: 60%

Isolation and Characterization of Newly Laccase-Producing Endophytic Fungi in Submerged Cultures from Calotropis gigantean Plant Leaves

Naz,

Gupta,

Chatterjee

2023

IJPE

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The aim of the study to isolate and identify new laccase sources from an endophytic fungal source that could be used as a weapon foreliminating and detoxifying contaminants found in wastewater and aquatic habitats. Isolation was done from Calotropis gigantean plantleaves from different locations of paper mill effluents from the “Raipur” region of Chhattisgarh, India. Positive isolates were obtainedwith a dark brown color below and surrounding the fungal colony due to guaiacol oxidation on potato dextrose agar. One potentendophytic fungal isolate that produces laccase is identified as Aspergillus turcosus by using the internal transcribed spacer (ITS) andBLAST analysis. After optimization, maximum laccase production was obtained at the following conditions: medium (Czapeck Dox Broth),carbon source (sucrose), nitrogen source (sodium nitrate), pH (6), activator tannic acid (20 mM), incubation period (35°C) and duration(8 days) with 3 (8 mM fermentation) disc inoculums. The maximum laccase activity was obtained at 65 U mL-1 in submerged optimizedconditions, which was more than two fold compared to the unoptimized conditions. As estimated by SDS-PAGE, The molecular massof the monomer of pure laccase was determined to be 66 kDa. After five days of treatment with the laccase of A. turcosus, the syntheticdyes phenol red, bromophenol blue, methyl orange, and Congo red lose their colour. Clearance rates for chemical oxygen demand were59.46 and 48.57%, and phenolic contaminants were 80 and 22.3% in coal and textile effluents during the required treatment periods,respectively. One novel and potent laccase-producing endophytic fungus was successfully isolated, which can be utilized as a laccaseproducingsource for various industrial applications.

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“…To identify the optimal incubation temperature, a wide range of temperatures (−20 °C, 0 °C, 20 °C, 25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C, and 60 °C) was examined for both free and conjugated enzymes. The assay mixture contained 150 μL of 0.5 mM substrate (ABTS-2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid), 2.7 ml of 0.1 M sodium acetate buffer of pH 4.5 and 150 μl of enzyme (at a concentration of 50 U ml −1 )/enzyme-nanoparticle conjugates and was incubated for 5 min (Sidhu et al 2017). For pH optimization, same enzyme assay method was followed with various buffers spanning a broad pH, including pH 2 Citrate phosphate buffer, pH 3 Citrate buffer, pH 4 Citrate buffer, pH 5 sodium acetate buffer, pH 6 phosphate buffer, pH 7 phosphate buffer, and pH 8 glycine NaOH buffer, offering a comprehensive assessment of enzyme activity across the pH spectrum.…”

Section: Characterization Of Synthesized Magnetic Nanoparticlesmentioning

confidence: 99%

Direct binding and characterization of laccase onto iron oxide nanoparticles

Sidhu,

Patil,

Gaikwad

2024

Nanotechnology

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Iron oxide nanoparticles exhibit unique magnetic properties and possess a high surface-to-volume ratio, making them ideal candidates for the conjugation of substances, including enzymes. Laccase (EC 1.10.3.2), an oxidative enzyme with diverse applications, presents an opportunity for enhancing stability and reusability through innovative immobilization techniques, thus reducing overall process costs. In this study, we employed a direct binding procedure via carbodiimide activation to conjugate laccase onto iron oxide nanoparticles synthesized using thermal chemical coprecipitation. Stabilization of the nanoparticles was achieved using thioglycerol and polyvinyl chloride as capping agents. Characterization of the synthesized nanoparticles was conducted using UV-spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Energy dispersive X-ray spectroscopy (EDS). FTIR spectroscopy analysis confirmed successful laccase binding to magnetic nanoparticles, with binding efficiencies of 90.65% and 73.02% observed for thioglycerol and polyvinyl chloride capped iron oxide nanoparticles, respectively. Furthermore, the conjugated enzyme exhibited remarkable stability, retaining nearly 50% of its initial activity after 20 reuse cycles. This research demonstrates that immobilizing laccase onto iron oxide nanoparticles enhances its activity, stability, and reusability, with the potential for significant cost savings and expanded applications in various fields.

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Isolation, Screening and Optimization for Laccase production by Scytalidium lignicola pesante under submerged fermentation

Cited by 6 publications

References 11 publications

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Isolation and Characterization of Newly Laccase-Producing Endophytic Fungi in Submerged Cultures from Calotropis gigantean Plant Leaves

Direct binding and characterization of laccase onto iron oxide nanoparticles

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