Isolation, structure, and bioactivities of polysaccharides from <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja

Li, Qiqiong; Hu, Jielun; Xie, Jianhua; Nie, Shaoping; Xie, Mingyong

doi:10.1111/nyas.13357

Cited by 32 publications

(19 citation statements)

References 36 publications

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“…Cyclocarya paliurus is a plant of the genus Chrysanthemum (Jugaceae), mainly distributed in southern China. Its branches and leaves are sweet, having a cooling effect, and can reduce swelling and pain [5]. Modern pharmacological studies have shown that Cyclocarya paliurus contains a variety of nutrients and can play different biological roles [6].…”

Section: Introductionmentioning

confidence: 99%

Cyclocarya paliurus Polysaccharide Inhibits Glioma Cell U251 Proliferation, Migration, and Invasion and Promotes Apoptosis via the GSK3β/β-Catenin Signaling Pathway

Guo

et al. 2020

International Journal of Polymer Science

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Objective. To investigate the effects of Cyclocarya paliurus polysaccharide (CPP) on the proliferation, migration, invasion, and apoptosis of human glioma U251 cells and further explore the underlying mechanism. Methods. U251 cells were cultured in vitro and treated with various concentrations (25, 50, 75, 100, 125, and 150 μmol/L) of CPP for 24, 48, and 72 h. Cell counting kit-8 was used to detect the activity of cell proliferation. Wound-healing assay, Transwell assay, and flow cytometry were used to measure the effects of CPP on the migration, invasion, and apoptosis of U251 cells, respectively. Western blotting was used to determine the protein expression involved in the GSK3β/β-catenin signaling pathway and its downstream genes related to proliferation, migration, invasion, and apoptosis including Cyr61, CCND1, Vimentin, and Slug. Meanwhile, qRT-PCR was used to detect the mRNA levels of Cyr61, CCND1, Vimentin, and Slug. Results. We found that CPP not only could inhibit the proliferation, migration, and invasion of U251 cells but also promote its apoptosis in vitro. Besides, CPP could significantly inhibit the phosphorylation and decrease the protein levels of GSK3 β at ser9 site (p<0.05), and thus increasing the phosphorylation of β-Catenin at ser33/37 site (p<0.05), resulting in β-Catenin degradation. In addition, we also found that CPP could downregulate the mRNA (p<0.05) and protein expression (p<0.05) of downstream genes of GSK3 β/β-catenin signaling pathway including Cyr61, CCND1, Vimentin, and Slug, which are related to proliferation, migration, invasion, and apoptosis. Conclusion. CPP could inhibit the expression of GSK3β, promote the degradation of β-catenin, and downregulate the levels of GSK3β/β-catenin downstream genes including Cyr61, CCND1, Vimentin, and Slug, which regulate the proliferation, migration, invasion, and apoptosis of glioma cells.

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Section: Introductionmentioning

confidence: 99%

Cyclocarya paliurus Polysaccharide Inhibits Glioma Cell U251 Proliferation, Migration, and Invasion and Promotes Apoptosis via the GSK3β/β-Catenin Signaling Pathway

Guo

et al. 2020

International Journal of Polymer Science

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“…It is one of the national key protected endangered plants in China [1–3]. It was found that CP contains a variety of biological active ingredients, such as polysaccharides, flavonoids, saponins, and triterpenes [3–5], and thus has the pharmacological effects of hypoglycemia [6], lowering blood pressure [7], and hypolipidemia [8–11]. In addition, previous studies showed that C. paliurus aqueous extract inhibited α-glucosidase activity and decreased blood glucose level in diabetic mice [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Cyclocarya Paliurus on Hypoglycemic Effect in Type 2 Diabetic Mice

Zhao

Wang

et al. 2019

Med Sci Monit

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Background The aim of this study was to assess the hypoglycemic effect of Cyclocarya paliurus extract (CPE) on diabetes mellitus (DM) mice. Material/Methods A DM mouse model was established to test FBG, TC, and TG. The DM mice were divided into 3 groups: a DM group, a DM+CPE (0.5 g/Kg) group, and a DM+CPE (1.0 g/Kg) group. The FBG and body weight were measured. The glucose tolerance ability was determined by OGTT test. FINS was measured to calculate ISI and IRI. Serum MDA, SOD, and GSH-Px levels were detected. NIT-1 cells were cultured in vitro and divided into 4 groups: a control group, a STZ group, a STZ+CPE (80 μg/mL) group, and a STZ+CPE (160 μg/mL) group. Cell apoptosis and ROS content were assessed by flow cytometry. Cell proliferation was detected by EdU staining. Results Compared with the control group, FBG, TC, and TG were significantly increased in the DM group. CPE gavage obviously reduced FBG level, increased body weight, enhanced glucose tolerance, elevated FINS level and ISI, and reduced IRI, all in a dose-dependent manner. CPE gavage reduced serum MDA content and increased SOD and GSH-Px enzyme activities in DM mice. STZ markedly enhanced ROS production, induced apoptosis, and inhibited proliferation in NIT-1 cells. CPE treatment clearly reduced ROS production and apoptosis, enhanced cell proliferation, and alleviated STZ damage to NIT-1 cells. Conclusions CPE has the effects of decreasing blood glucose and insulin resistance, and enhancing glucose tolerance in DM mice, which may be related to its effects of reducing oxidation and reduced apoptosis, and relieving STZ in pancreatic beta cell injury.

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“…Iljinsk ( C. paliurus ), a plant of the genus Cyclocarya (Juglandaceae), is the sole species in its genus and is unique to China (8). It is mainly distributed in Southern China and grows at 420–2,500 m altitude in mountainous humid evergreen forests (8,9). Its branches and leaves taste sweet, have a cooling effect, and may reduce swelling and pain (8).…”

Section: Introductionmentioning

confidence: 99%

“…It is mainly distributed in Southern China and grows at 420–2,500 m altitude in mountainous humid evergreen forests (8,9). Its branches and leaves taste sweet, have a cooling effect, and may reduce swelling and pain (8). From its leaves, Chinese populations produce health tea, commonly termed ‘sweet tea’.…”

Section: Introductionmentioning

confidence: 99%

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Antiproliferative and pro‑apoptotic effects of Cyclocarya�paliurus polysaccharide and X‑ray irradiation combination on SW480 colorectal cancer cells

Jin

Jiang

2019

Mol Med Report

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The anti-hyperglycemic effects of Cyclocarya paliurus polysaccharide (CPP) have attracted increasing attention; however, limited research has been conducted on the potential effects of CPP on inhibiting tumor growth. The present study aimed to investigate the functions of CPP in combination with X-ray irradiation on colorectal cancer cells and the underlying mechanisms. SW480 cells were treated with various concentrations of CPP for 24, 48 and 72 h to determine cell viability using a Cell Counting Kit-8 assay. Then, the cells were divided into four groups as follows: Control, CPP (100 µmol/l), 8 Gy and CPP + 8 Gy. The proliferation and apoptosis, and colony formation of cells were detected using flow cytometry and plate clone formation assays, respectively. Reverse transcription-quantitative PCR and western blot analyses were conducted to determine the expression of proliferation and apoptosis-associated, and PI3K/Akt signaling-associated genes. Treatment with 75 µmol/l CPP for 48 h significantly decreased cell viability compared with untreated cells. CPP in combination with 8 Gy X-ray treatment significantly promoted the induction of apoptosis, and suppressed cell proliferation and clone formation compared with the control, CPP and 8 Gy groups. The detection of mRNA and protein expression levels by reverse transcription-PCR and western blotting demonstrated that CPP in combination with 8 Gy not only significantly decreased the expression of proliferation marker protein Ki-67, p53 and Bcl-2, but also upregulated the expression of cleaved caspase-3 and Bax, compared with the control. In addition, CPP and 8 Gy combined significantly attenuated the phosphorylation of PI3K and Akt. The present study demonstrated that the combination of CPP with X-ray irradiation suppressed SW480 cell proliferation and promoted cell apoptosis compared with the control, CPP and 8 Gy groups. The underlying mechanisms may involve inhibition of PI3K/Akt signaling.

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Isolation, structure, and bioactivities of polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja

Cited by 32 publications

References 36 publications

Cyclocarya paliurus Polysaccharide Inhibits Glioma Cell U251 Proliferation, Migration, and Invasion and Promotes Apoptosis via the GSK3β/β-Catenin Signaling Pathway

Cyclocarya paliurus Polysaccharide Inhibits Glioma Cell U251 Proliferation, Migration, and Invasion and Promotes Apoptosis via the GSK3β/β-Catenin Signaling Pathway

Effect of Cyclocarya Paliurus on Hypoglycemic Effect in Type 2 Diabetic Mice

Antiproliferative and pro‑apoptotic effects of Cyclocarya�paliurus polysaccharide and X‑ray irradiation combination on SW480 colorectal cancer cells

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