Isoprenoids Are Essential for Fruiting Body Formation in
            <i>Myxococcus xanthus</i>

Lorenzen, Wolfram; Ring, Michael W.; Schwär, Gertrud; Bode, Helge B.

doi:10.1128/jb.00539-09

Cited by 5 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the formation of acetyl-coA requires energy that is provided by pyruvate decarboxylation, the former pathway may be advantageous if prey-derived (aceto)acetyl-coA is available. It is noteworthy that almost all free-living non-predators, that may be potential prey, but not (Handa et al, 2005) were significantly more abundant in the BALOs, where they are upregulated during growth (Lambert et al, 2010;Wang et al, 2011); also, in myxobacteria a new pathway that branches from 3-hydroxy-3-methylglutaryl-CoA forms isovaleryl coenzyme A and compounds derived thereof, which are essential for fruiting body formation (Lorenzen et al, 2009). These examples of differential enrichment for a metabolic pathway, as well as for a particular tRNA synthetase gene (see above) in phylogenetically unrelated bacterial species suggest that they have been independently selected for and that they may confer as yet unknown selective advantages.…”

Section: Discussionmentioning

confidence: 99%

By their genes ye shall know them: genomic signatures of predatory bacteria

Pasternak

Pietrokovski

Rotem

et al. 2012

ISME J

View full text Add to dashboard Cite

Predatory bacteria are taxonomically disparate, exhibit diverse predatory strategies and are widely distributed in varied environments. To date, their predatory phenotypes cannot be discerned in genome sequence data thereby limiting our understanding of bacterial predation, and of its impact in nature. Here, we define the 'predatome,' that is, sets of protein families that reflect the phenotypes of predatory bacteria. The proteomes of all sequenced 11 predatory bacteria, including two de novo sequenced genomes, and 19 non-predatory bacteria from across the phylogenetic and ecological landscapes were compared. Protein families discriminating between the two groups were identified and quantified, demonstrating that differences in the proteomes of predatory and non-predatory bacteria are large and significant. This analysis allows predictions to be made, as we show by confirming from genome data an over-looked bacterial predator. The predatome exhibits deficiencies in riboflavin and amino acids biosynthesis, suggesting that predators obtain them from their prey. In contrast, these genomes are highly enriched in adhesins, proteases and particular metabolic proteins, used for binding to, processing and consuming prey, respectively. Strikingly, predators and non-predators differ in isoprenoid biosynthesis: predators use the mevalonate pathway, whereas non-predators, like almost all bacteria, use the DOXP pathway. By defining predatory signatures in bacterial genomes, the predatory potential they encode can be uncovered, filling an essential gap for measuring bacterial predation in nature. Moreover, we suggest that fullgenome proteomic comparisons are applicable to other ecological interactions between microbes, and provide a convenient and rational tool for the functional classification of bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

By their genes ye shall know them: genomic signatures of predatory bacteria

Pasternak

Pietrokovski

Rotem

et al. 2012

ISME J

View full text Add to dashboard Cite

show abstract

“…Our findings imply a link between membrane order and protein function in prokaryotes. They also suggest a possible lipid target to address bacterial multidrug resistance, further implicating isoprenoidal lipid biosynthesis as a bacterial Achilles heel (25,26).…”

Section: Significancementioning

confidence: 99%

Hopanoids as functional analogues of cholesterol in bacterial membranes

Sáenz

Grosser

Bradley

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

213

177

View full text Add to dashboard Cite

The functionality of cellular membranes relies on the molecular order imparted by lipids. In eukaryotes, sterols such as cholesterol modulate membrane order, yet they are not typically found in prokaryotes. The structurally similar bacterial hopanoids exhibit similar ordering properties as sterols in vitro, but their exact physiological role in living bacteria is relatively uncharted. We present evidence that hopanoids interact with glycolipids in bacterial outer membranes to form a highly ordered bilayer in a manner analogous to the interaction of sterols with sphingolipids in eukaryotic plasma membranes. Furthermore, multidrug transport is impaired in a hopanoid-deficient mutant of the gram-negative Methylobacterium extorquens, which introduces a link between membrane order and an energy-dependent, membrane-associated function in prokaryotes. Thus, we reveal a convergence in the architecture of bacterial and eukaryotic membranes and implicate the biosynthetic pathways of hopanoids and other order-modulating lipids as potential targets to fight pathogenic multidrug resistance.

show abstract

“…[73] Myxobacteria are known producers of terpenoids. [74][75] In the genome of S. cellulosum So ce56, a terpene pathway gene cluster is also evident, [53] thus probably highlighting a need for the TS product. As myxobacteria are known to form fruiting bodies upon starvation, [76] single cells need to communicate in order to facilitate the complex lifestyle, [77] and the emission and sensing of such volatile terpenes could be a mechanism of chemical communication.…”

Section: Resultsmentioning

confidence: 98%

Characterization of the Gene Cluster CYP264B1‐geoA from Sorangium cellulosum So ce56: Biosynthesis of (+)‐Eremophilene and Its Hydroxylation

Schifrin

Günnewich

et al. 2014

ChemBioChem

View full text Add to dashboard Cite

Terpenoids can be found in almost all forms of life; however, the biosynthesis of bacterial terpenoids has not been intensively studied. This study reports the identification and functional characterization of the gene cluster CYP264B1-geoA from Sorangium cellulosum So ce56. Expression of the enzymes and synthesis of their products for NMR analysis and X-ray diffraction were carried out by employing an Escherichia coli whole-cell conversion system that provides the geoA substrate farnesyl pyrophosphate through simultaneous overexpression of the mevalonate pathway genes. The geoA product was identified as a novel sesquiterpene, and assigned NMR signals unambiguously proved that geoA is an (+)-eremophilene synthase. The very tight binding of (+)-eremophilene (∼0.40 μM), which is also available in S. cellulosum So ce56, and its oxidation by CYP264B1 suggest that the CYP264B1-geoA gene cluster is required for the biosynthesis of (+)-eremophilene derivatives.

show abstract

Isoprenoids Are Essential for Fruiting Body Formation in Myxococcus xanthus

Cited by 5 publications

References 30 publications

By their genes ye shall know them: genomic signatures of predatory bacteria

By their genes ye shall know them: genomic signatures of predatory bacteria

Hopanoids as functional analogues of cholesterol in bacterial membranes

Characterization of the Gene Cluster CYP264B1‐geoA from Sorangium cellulosum So ce56: Biosynthesis of (+)‐Eremophilene and Its Hydroxylation

Contact Info

Product

Resources

About