Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

Parkhahyun,; Song, Gwonhwa; Lim, Whasun

doi:10.12750/jarb.36.1.17

Cited by 16 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, 200 μM PA significantly increased tiRNA expression in trophoblasts. Differences in the expression of tiRNAs depending on the concentration of PA require further study; however, a recent study on angiogenin-mediated inflammation-induced metabolic disease suggests a new link between tiRNA generation and inflammation (Park et al, 2021;Zhang et al, 2021). Angiogenin expression is increased in the mouse caput epididymis by LPS, and inflammation is inhibited in mice lacking angiogenin, accompanied by a change in the tRNA-derived species profile.…”

Section: Discussionmentioning

confidence: 99%

Palmitic acid induces inflammatory cytokines and regulates tRNA-derived stress-induced RNAs in human trophoblasts

Yang

Song

et al. 2022

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

High levels of proinflammatory cytokines have been observed in obese pregnancies. Obesity during pregnancy may increase the risk of various pregnancyrelated complications, with pathogenesis resulting from excessive inflammation. Palmitic acid (PA) is a saturated fatty acid that circulates in high levels in obese women. In our previous study, we found that PA inhibited the proliferation of trophoblasts developing into the placenta, induced apoptosis, and regulated the number of cleaved halves derived from transfer RNAs (tRNAs). However, it is not known how the expression of tRNA-derived stress-induced RNAs (tiRNAs) changes in response to PA treatment at concentrations that induce inflammation in human trophoblasts. We selected concentrations that did not affect cell viability after dose-dependent treatment of HTR8/SVneo cells, a human trophoblast cell line. PA (200 μM) did not affect the expression of apoptotic proteins in HTR8/SVneo cells. PA significantly increased the expression of inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α. In addition, 200 μM PA significantly increased the expression of tiRNAs compared to 800 μM PA treatment. These results suggest that PA impairs placental development during early pregnancy by inducing an inflammatory response in human trophoblasts. In addition, this study provides a basis for further research on the association between PA-induced inflammation and tiRNA generation.

show abstract

Section: Discussionmentioning

confidence: 99%

Palmitic acid induces inflammatory cytokines and regulates tRNA-derived stress-induced RNAs in human trophoblasts

Yang

Song

et al. 2022

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

show abstract

“…Adult wild-type (AB strain) and transgenic (flk1:eGFP) zebrafish lines acquired from the Zebrafish Organogenesis Mutant Bank (Kyungpook National University, Korea) were maintained as previously reported (Park et al, 2021).…”

Section: Experimental Organismmentioning

confidence: 99%

Norflurazon causes developmental defects including cardiovascular abnormalities in early-stage zebrafish (Danio rerio)

Park

Hong

et al. 2022

J Anim Reprod Biotechnol

Self Cite

View full text Add to dashboard Cite

Norflurazon is widely used on agricultural lands and has a high potential to pollute water sources. However, its effects on fish have not been fully elucidated. The purpose of our study was to determine whether norflurazon adversely affects the developmental stage of zebrafish, which are frequently used as a model system to evaluate the environmental impact of pollutants. Norflurazon interfered with the hatching of zebrafish embryos and induced several sublethal deformities including body length reduction, increased yolk sac volume, and enlargement of the pericardial region. We further examined the cardiotoxicity of norflurazon in the flk1:eGFP transgenic zebrafish line. The vascular network, mainly in the brain region, was significantly disrupted in norflurazon-exposed zebrafish. In addition, due to the failure of cardiac looping, norflurazon-exposed zebrafish had an abnormal cardiac structure. These developmental abnormalities were related to the apoptotic process triggered by norflurazon. Overall, the present study demonstrated the non-target toxicity of norflurazon by analyzing the hazardous effects of norflurazon on developing zebrafish.

show abstract

“…Despite the extensive use of picolinafen, its molecular mechanisms in non-target cells have not been extensively studied. Previous studies have shown high bioaccumulation and reproductive toxicity of herbicides in sh (An et al 2021;Park et al 2021;Yang et al 2021). In addition, research on the toxic effects of herbicides in murine preimplantation embryos revealed that exposure to low-dose herbicides increased apoptosis and inhibited blastocyst development (Greenlee et al 2004).…”

Section: Introductionmentioning

confidence: 98%

Picolinafen exposure induces ROS accumulation and calcium depletion, leading to apoptosis in porcine embryonic trophectoderm and uterine luminal epithelial cells

Park¹,

Park²,

Lim

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

The global use of herbicides accounts for more than 48% of total pesticide usage. Picolinafen is a pyridine carboxylic acid herbicide that is predominantly used to control broadleaf weeds in wheat, barley, corn, and soybeans. Despite its widespread use in agriculture, its toxicity in mammals has rarely been studied. In this study, we first identified the cytotoxic effects of picolinafen on porcine trophectoderm (pTr) and luminal epithelial (pLE) cells, which are involved in the implantation process during early pregnancy. Picolinafen treatment significantly decreased the viability of pTr and pLE cells. Our results demonstrate that picolinafen increased the number of sub-G1 phase cells and early/late apoptosis. In addition, picolinafen disrupted mitochondrial function and resulted in the accumulation of intracellular ROS, leading to a reduction in calcium levels in both the mitochondria and cytoplasm of pTr and pLE cells. Moreover, picolinafen was found to significantly inhibit the migration of pTr. These responses were accompanied by the activation of the MAPK and PI3K signal transduction pathways by picolinafen. Our data suggest that the deleterious effects of picolinafen on the viability and migration of pTr and pLE cells might impair their implantation potential.

show abstract

Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

Cited by 16 publications

References 27 publications

Palmitic acid induces inflammatory cytokines and regulates tRNA-derived stress-induced RNAs in human trophoblasts

Palmitic acid induces inflammatory cytokines and regulates tRNA-derived stress-induced RNAs in human trophoblasts

Norflurazon causes developmental defects including cardiovascular abnormalities in early-stage zebrafish (Danio rerio)

Picolinafen exposure induces ROS accumulation and calcium depletion, leading to apoptosis in porcine embryonic trophectoderm and uterine luminal epithelial cells

Contact Info

Product

Resources

About