Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis

Janicka, Monika; Kot‐Wasik, Agata; Kot, Jacek; Namieśnik, Jacek

doi:10.3390/ijms11114631

Cited by 103 publications

(60 citation statements)

References 103 publications

(103 reference statements)

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“…8-isoPGF 211 is a key biomarker for evaluation of free radical generation in the pathogenesis ofhuman diseases (28), and an increased lung tissue level of 8-isoPGF 211 has been reported in various pulmonary diseases (29). Production of 8-isoPGF 211 has been detected in whole lung Iysates, while inhibition of the ROCK pathway reduces the 8-isoPGF 211level in whole lung Iysates from a guinea pig model of allergy based on ovalbumin inhalation (30).…”

Section: Discussionmentioning

confidence: 99%

Rock Activation in Lung of Idiopathic Pulmonary Fibrosis with Oxidative Stress

Shimizu

Dobashi

Sano

et al. 2014

Int J Immunopathol Pharmacol

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The Rho-associated coiled-coil containing protein kinase, (Rho-kinase or ROCK) undergoes activation by oxidative stress. ROCK-II, which is an isoform of ROCK, is activated in a murine model of lung fibrosis. The present study evaluated the level of oxidative stress and activation of ROCK-II in patients with idiopathic pulmonary fibrosis (IPF). The ROCK-II level and the phosphorylation of myosin phosphatase subunit-1 (p-MYPT-1), a hallmark of ROCK activation, were examined by immunohistochemistry of lung tissue sections. The 8-iso prostaglandin-F2α (8-isoPGF2α) level, as a marker of oxidative stress, of exhaled breath condensate was significantly higher in IPF patients than in control patients. In IPF lungs, ROCK-II was predominantly expressed by bronchial epithelial cells, as well as at a lower level by airway smooth muscle cells, vascular smooth muscle cells, and the fibroblasts of fibroblastic foci (FF). In addition, there was moderate p-MYPT-1 expression in these cells of IPF lungs. In control lungs, ROCK-II was expessed by these cells. p-MYPT-1 was weakly expressed by the bronchial epithelial cells. In conclusion, ROCK-II was activated in various lung cells of IPF patients along with oxidative stress detected by 8-isoPGF2α elevation. The ROCK pathway may play a role in the development of IPF via oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Rock Activation in Lung of Idiopathic Pulmonary Fibrosis with Oxidative Stress

Shimizu

Dobashi

Sano

et al. 2014

Int J Immunopathol Pharmacol

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“…Therefore, F2-isoprostanes have been regarded as reliable biomarkers of oxidative stress. Furthermore, F2-isoprostanes have been shown to exert potent vasoconstrictor effects on animal and human vessels, suggesting a pathogenic role in cardiovascular diseases and have been extensively used as markers of lipid peroxidation in human diseases [74,75,148]. Their high stability and presence in measurable concentrations in many biological tissues and fluids, under physiological and pathological conditions, has also allowed the establishment of reference intervals and the comparison or monitoring of disease states [97,149,150].…”

Section: F2-isoprostanesmentioning

confidence: 99%

Lipid Peroxidation and Antioxidants in Arterial Hypertension

Sousa¹,

Afonso²,

Albino‐Teixeira³

et al. 2012

Lipid Peroxidation

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“…v-3 PUFAs mainly generate F 3 and F 4 -isoprostanes [28]. F 2 -isoprostanes are considered as reliable biomarkers of lipid peroxidation in various inflammatory states, such as atherosclerosis, rheumatic diseases [48], chronic lung diseases [49], degenerative diseases, aging, obesity and cancers [50]. In obesity, one of the risk factors of CRC, F 2 -isoprostanes level is highly correlated with visceral and subcutaneous adipose tissue.…”

Section: Isoprostanes: In-vivo Golden Standard Biomarker Of Lipid Permentioning

confidence: 99%

Role of polyunsaturated fatty acids and lipid peroxidation on colorectal cancer risk and treatments

Cai

Dupertuis

Pichard

2012

Current Opinion in Clinical Nutrition and Metabolic Care

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Recent findings CRC is one of the most overriding threats to public health. Despite a broad range of treatments, up to 50% of patients will inevitably develop incurable metastatic disease. Peroxidation of PUFAs contributes to augmentation of oxidative stress and causes in consequence inflammation, which is one of the possible carcinogenic factors of CRC. End products of PUFAs might be used as biomarkers for CRC detection and surveillance for treatment. They also have cytotoxic effect in CRC cells. Experimental results suggest that v-3 PUFAs could increase the efficacy of radiotherapy and chemotherapy of CRC. SummaryLipid peroxidation, one factor of oxidative stress, might play a paramount role not only in carcinogenesis but also in potential therapeutic strategy on CRC. End products of lipid peroxidation, such as malondialdehyde, 4-hydroxy-2-nonenal, and isoprostanes, could be used as biomarkers for cancer detection, surveillance of treatment outcome and prognostic index for CRC patients. Furthermore, malondialdehyde and 4-hydroxy-2-nonenal have cytotoxic effect not only in normal cells but also in CRC cancer cells, which implies the potential role of PUFAs in CRC treatment.

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Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis

Cited by 103 publications

References 103 publications

Rock Activation in Lung of Idiopathic Pulmonary Fibrosis with Oxidative Stress

Rock Activation in Lung of Idiopathic Pulmonary Fibrosis with Oxidative Stress

Lipid Peroxidation and Antioxidants in Arterial Hypertension

Role of polyunsaturated fatty acids and lipid peroxidation on colorectal cancer risk and treatments

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