Isoquinoline alkaloid fractions of Fumaria officinalis: Characterization and evaluation of their antioxidant and antibacterial activities

Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry

“…These results agreed with previous studies on this plant using GC coupled to a quadrupole mass analyzer [ 11 , 12 ] and other studies [ 9 , 25 ], while additionally we also detect coreximine. Moreover, in agreement with previous studies [ 13 , 14 , 17 , 24 ] our results suggest that the stationary phase 5% phenyl 95% dimethyl polysiloxane is also an adequate alternative to the more apolar stationary phases consisting of 100% dimethyl polysiloxane, which is also commonly used for the separation of isoquinoline alkaloids [ 11 , 12 ].…”

“…Briefly, for the untargeted analysis of the alkaloidal extract via GC-TOF-MS the strategy followed consisted of the generation of the molecular formulae of the detected ions in the chromatographic profile ( Figure 1(a) ) and studying the fragmentation pattern using NIST MS library when possible or based on literature [ 11 , 12 , 14 , 24 ]. The results provided by the GC–TOF-MS analyzer are given in Table 1 , which shows MS experimental data, retention time (RT), and main fragments generated by EI from F. capreolata alkaloids.…”

“…It seems that this platform is highly attractive since it can provide not only the fragmentation pattern of alkaloids, but also their molecular formulae, a basic clue for structure elucidation of unknown compounds that are not available in current GC-MS libraries. This was the case of dihydrosanguinarine and parfumine (Table S1), whose structures were proposed based on both their molecular formula and the fragmentation pattern that could be fortunately compared with literature [ 11 , 14 , 24 ].…”

Evaluation of Polyphenolic Content, Antioxidant and Diuretic Activities of Six Fumaria Species

“…Various species have been reported for their use in traditional medicine for the treatment of hepatobiliary pathologies [ 5 ]. Other sources cite the antifungal [ 6 ], antibacterial [ 7 ], and anti-inflammatory [ 8 ] activities of these species. These activities are especially due to the isoquinolinic alkaloids, among which protopine is the most frequently found [ 9 ].…”

“…An important number of biological activities are associated and assessed to the Fumaria officinalis L. ( Figure 1 ) [ 17 , 18 ], a species that is also spontaneous in the Western part of Europe [ 2 ]. The antibacterial [ 7 ] and the antioxidant [ 19 ] activities are the most frequently cited by scientific literature. Compounds that are responsible for these activities are mostly isoquinolinic alkaloids, but there are also authors that state the involvement of the polyphenols in some of these activities [ 11 ].…”