Isorhamnetin and its new derivatives isolated from sea buckthorn berries prevent H2O2/Fe – Induced oxidative stress and changes in hemostasis

Skalski, Bartosz; Lis, Bernadetta; Pecio, Łukasz; Kontek, Bogdan; Olas, Beata; Żuchowski, Jerzy; Stochmal, Anna

doi:10.1016/j.fct.2019.02.014

Cited by 40 publications

(45 citation statements)

References 41 publications

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“…The wide range of applied concentrations of the crude extract, the phenolic fraction, and phenolic compounds isolated from lentil aerial parts (1-50 µg/mL) was in accordance with the general practice in in vitro model [11,14,17]. Besides, the concentration range used in studies was the same as in our earlier studies to maintain the continuity of research [8].…”

Section: Discussionsupporting

confidence: 55%

“…Comparable lipid peroxidation experiments using blood plasma and H 2 O 2 /Fe-induced oxidative stress are also not common. However, our earlier work on sea buckthorn fruit flavonoids also demonstrated that two isorhamnetin glycosides exerted a similar and stronger inhibitory effect on plasma lipid peroxidation as compared to their aglycone [14].…”

Section: Discussionmentioning

confidence: 85%

“…The carbonyl group concentration was calculated using a molar extinction coefficient (ε = 22,000 M −1 cm −1 ) and the level of carbonyl groups as nmol carbonyl groups/mg of plasma protein. More details on the method are described in other papers [12][13][14].…”

Section: Carbonyl Group Measurementmentioning

confidence: 99%

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Modulation of Oxidative Stress and Hemostasis by Flavonoids from Lentil Aerial Parts

et al. 2021

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While specific metabolites of lentil (Lens culinaris L.) seeds and their biological activity have been well described, other organs of this plant have attracted little scientific attention. In recent years, green parts of lentils have been shown to contain diverse acylated flavonoids. This work presents the results of the research on the effect of the crude extract, the phenolic fraction, and seven flavonoids obtained from aerial parts of lentils on oxidative damage induced by H2O2/Fe to lipid and protein constituents of human plasma. Another goal was to determine their effect on hemostasis parameters of human plasma in vitro. Most of the purified lentil flavonoids had antioxidant and anticoagulant properties. The crude extract and the phenolic fraction of lentil aerial parts showed antioxidant activity, only at the highest tested concentration (50 μg/mL). Our results indicate that aerial parts of lentils may be recommended as a source of bioactive substances.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 85%

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Modulation of Oxidative Stress and Hemostasis by Flavonoids from Lentil Aerial Parts

et al. 2021

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“…The TBARS concentration was calculated using the molar extinction coefficient (ε = 156,000 M − 1 cm − 1 ). More details were described in Skalski et al [21].…”

Section: Methodsmentioning

confidence: 99%

Biological properties of Elaeagnus rhamnoides (L.) A. Nelson twig and leaf extracts

Skalski

Kontek

Lis

et al. 2019

BMC Complement Altern Med

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Background Sea buckthorn ( Elaeagnus rhamnoides (L.) A. Nelson, SBT) is a valuable plant because of its medical and therapeutic potential. Different bioactive compounds in SBT berries are of special interest to various researchers. However, not only sea buckthorn berries, but also leaves of this plant (both fresh and dried) contain a lot of nutrients and bioactive compounds, including phenolic compounds. The present study was carried out in order to investigate antioxidant and anticoagulant properties of sea buckthorn twig and leaf extracts (0.5–50 μg/mL) by using various in vitro models. Moreover, the aim of present experiments was to compare the biological activity of SBT leaf extract and SBT twig extract with selected berry extracts (a rich source of phenolic compounds): SBT berry extract (flavonoids being the dominant components), a commercial extract from the berries of Aronia melanocarpa (Aronox ® ), and a grape seed extract. Methods We determined the effect of plant extracts on the oxidative stress using selected markers of this process, i.e. the level of carbonyl groups in proteins. Additionally, we analysed the potential mechanism of modulation of hemostatic properties of human plasma (using selected coagulation times). Results SBT twig and leaf extracts were observed to exhibit an antioxidant activity against two strong biological oxidants: hydrogen peroxide (H 2 O 2 ) and H 2 O 2 /Fe (the donor of hydroxyl radicals), which induced human plasma lipid peroxidation and protein carbonylation. Both extracts also showed anticoagulant properties. Conclusions Our present results have demonstrated that extracts from different parts of SBT, especially berries and twigs, in comparison to well-known berries (aronia and grape), may also be viewed as a good source of active substances – antioxidants for pharmacological or cosmetic applications. Moreover, it is very important from an economic point of view to know that there is a possibility of obtaining phenolic compounds not only from the berries or leaves, but also from twigs, which constitute a production waste.

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“…In this study, the effective hemostatic fractions of E. fortunei were screened by measuring the bleeding time (BT), coagulation time (CT), blood coagulation indexes, and platelet aggregation effect. Tail vein bleeding and capillary tube method in mice are presently the most commonly used bleeding and clotting time for assessment [17,18]. The values of BT and CT are influenced by different reasons.…”

Section: Discussionmentioning

confidence: 99%

Evaluations of Hemostatic Activity of Ethanol Extract of Euonymus fortunei Aerial Parts

Ouyang

Shu

Qin

et al. 2021

JPRI

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Aim: To investigate the hemostatic effect of ethanol extracts of Euonymus fortunei and their different polar fractions, and to analyze the changes in intracellular calcium ion concentration in platelet cells. Methodology: Bleeding time and clotting time was assessed by the slide method and the tail-breaking method, respectively. Four coagulation indexes, including prothrombin time, activated partial thromboplastin time, thrombin time, and the content of fibrinogen, as well as platelet aggregation effect were also determined. The concentration of calcium ions in platelet cells treated by n-butanol fraction with good active hemostatic activity was also examined. Results: Compared with the control group, the n-butanol fraction of E. fortunei can not only significantly shorten the bleeding time and coagulation time, but also obviously shorten activated partial thromboplastin time. However, no significant difference was found on the content of fibrinogen. Platelet aggregation experiments show that the n-butanol fraction can increase the intracellular Ca2+ concentration of platelet cells, which may be related to the activation of downstream signals through the platelet membrane G protein-coupled receptor. Conclusion: Ethanol extract of E. fortunei has excellent hemostatic activity, which may be related to the increase of intracellular Ca2+ concentration caused by G protein-coupled receptor on the platelet membrane surface. E. fortunei could be a potential medicinal plant used for hemostatic treatment.

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Isorhamnetin and its new derivatives isolated from sea buckthorn berries prevent H2O2/Fe – Induced oxidative stress and changes in hemostasis

Cited by 40 publications

References 41 publications

Modulation of Oxidative Stress and Hemostasis by Flavonoids from Lentil Aerial Parts

Modulation of Oxidative Stress and Hemostasis by Flavonoids from Lentil Aerial Parts

Biological properties of Elaeagnus rhamnoides (L.) A. Nelson twig and leaf extracts

Evaluations of Hemostatic Activity of Ethanol Extract of Euonymus fortunei Aerial Parts

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