Isoscutellarein 8, 4′-Dimethyl ether glycosides as cytotoxic agents and chemotaxonomic markers in Kickxia aegyptiaca

Farid, Mai M.; Marzouk, Mona M.; El-Shabrawy, Mona; Salem, Maha; Mounier, Marwa M.; Hussein, Sameh R.

doi:10.1016/j.bcab.2019.101431

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…3 В результате исследований M. M. Farid и др. разработана БАД из растительного экстракта Kermia aegyptiaca [14]. Полученная БАД была исследована методами биотехнологии против двух линий опухолевых клеток человека: молочной железы (MCF-7) и толстой кишки (HCT-116).…”

Section: Discussionunclassified

Effect of Dietary Supplement from Lymphoid Tissue of Chickens on Cell Viability

Кольберг

Тихонова

Tikhonov

et al. 2021

Food Processing: Techniques and Technology

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Introduction. Today, dietary supplements are an integral part of human diet. Some of them are made of hydrolysates of animal origin. Biologically active additives of immunomodulatory action can prevent various diseases. The research objective was to develop a dietary supplement from the bursa of Fabricius obtained from broiler chickens and evaluate its effect on cell viability in culture. Study objects and methods. The study featured biologically active supplement obtained by enzymatic hydrolysis of the bursa of Fabricius, immature stem cells, and adult differentiated cells of human dermal fibroblasts, HeLa and MCF-7 cancer cells, and extract of the bursa of Fabricius. Results and discussion. The research resulted in a new technology of dietary supplement production from the bursa of Fabricius of broiler chickens. It included washing, cutting, homogenization, proteolytic enzyme fermentation, and ultrafiltration. When introduced into the culture of mesenchymal stem cells, the dietary supplement caused a slight decrease in the cell viability at concentrations of 25 and 50%, which indicated a possible cytotoxic effect of the extract on mesenchymal cells. The extract did not affect the viability of human fibroblast culture and caused no cytotoxic effect. In MCF-7 culture, the extract had a dose-dependent cytotoxic effect, which lowered the relative cell viability. Conclusion. The new dietary supplement based on the bursa of Fabricius of broiler chickens had a cytotoxic effect on stem cell cultures. However, it did not affect the cell viability and had no cytotoxic effect on human dermal fibroblasts. The effect depended on the cell culture. In the case of HeLa, the supplement stimulated proliferative activity, and in the case of MCF-7, it had a cytotoxic effect. Therefore, the new dietary supplement demonstrated some prospects as an active ingredient for various biologically active additives and immunomodulatory drugs.

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Section: Discussionunclassified

Effect of Dietary Supplement from Lymphoid Tissue of Chickens on Cell Viability

Кольберг

Тихонова

Tikhonov

et al. 2021

Food Processing: Techniques and Technology

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“…The detailed MTT assay was performed according to the reported procedures and reported in the supporting materials. [25,[55][56][57] IC 50 values were calculated for the selected compounds 6e, 6g, 7e-h, and 7n.…”

Section: Biological Assaysmentioning

confidence: 99%

Novel 2‐oxo‐2‐phenylethoxy and benzyloxy diaryl urea hybrids as VEGFR‐2 inhibitors: Design, synthesis, and anticancer evaluation

Ghannam

Kerdawy

Mounier

et al. 2022

Archiv der Pharmazie

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Two series of diaryl urea derivatives, 6a-k and 7a-n, were synthesized. All the newly synthesized compounds were tested against the NCI (US) cancer cell lines via SRB assay. The p-chloro-m-trifluoromethyl phenyl derivatives 6e-g and 7e-g showed the most potent cytotoxic activity with a GI 50 value range of 1.2-15.9 µM. Furthermore, the p-fluorobenzyloxy diaryl urea derivative 7g revealed the most potent cytotoxicity against eight cancer cell lines in the MTT assay with IC 50 values below 5 µM. Compounds 6a-k and 7a-n were tested for their vascular endothelial growth factor receptor-2 (VEGFR-2) kinase inhibitory activities. The p-chloro-mtrifluoromethyl diaryl urea benzyloxy derivatives 7e-i and the p-methoxydiaryl urea benzyloxy derivatives 7k, 7l, and 7n were found to be the most active compounds as VEGFR-2 inhibitors in the benzyloxy series 7, with an IC 50 range of 0.09-4.15 µM. In the 2-oxo-2-phenylethoxy series 6, compounds 6e-g and 6i were reported with IC 50 values of 0.94, 0.54, 2.71, and 4.81 µM, respectively. Moreover, compounds 7e and 7g induced apoptosis, causing cell cycle arrest in the G2/M phase. In addition, 7gshowed an antimigratory effect in A-375 cells and inhibited the VEGFR-2 expression in an immunohistofluorescence study. Molecular docking simulations on VEGFR-2 as well as ADME properties prediction were also performed.

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“…Kickxia species, including K. aegyptiaca, were documented as significant traditional plants in the treatment of vascular diseases, haemorrhoids, and wounds, along with their uses as laxatives, anti-diabetics, anti-scorbutics, diuretics, and, tonics agents [ 12 , 14 ]. The phytochemical characterization of this plant revealed that it is rich in flavonoids, phenolic acids, glycosides, and iridoids [ 7 , 15 , 16 ]. The different K. aegyptiaca extracts and their isolated metabolites were documented to have antioxidant activity [ 17 , 18 ], larvicidal activity [ 18 ], and cytotoxic activity [ 15 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…The phytochemical characterization of this plant revealed that it is rich in flavonoids, phenolic acids, glycosides, and iridoids [ 7 , 15 , 16 ]. The different K. aegyptiaca extracts and their isolated metabolites were documented to have antioxidant activity [ 17 , 18 ], larvicidal activity [ 18 ], and cytotoxic activity [ 15 , 19 ]. Flavonoids, pectolinarigenin, tangeretin, and gardenin were also isolated from K. aegyptiaca, which exhibited potential antiviral activity against SARS-CoV-2 [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of Kickxia aegyptiaca Essential Oil and Its Potential Antioxidant and Antimicrobial Activities

Abd‐ElGawad

El‐Amier

Bonanomi

et al. 2022

Plants

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The exploration of new bioactive compounds from natural resources as alternatives to synthetic chemicals has recently attracted the attention of scientists and researchers. To our knowledge, the essential oil (EO) of Kickxia aegyptiaca has not yet been explored. Thus, the present study was designed to explore the EO chemical profile of K. aegyptiaca for the first time, as well as evaluate its antioxidant and antibacterial activities, particularly the extracts of this plant that have been reported to possess various biological activities. The EO was extracted from the aerial parts via hydrodistillation and then characterized by gas chromatography-mass spectrometry (GC-MS). The extracted EO was tested for its antioxidant activity via the reduction in the free radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). In addition, the EO was tested as an antibacterial mediator against eight Gram-negative and Gram-positive bacterial isolates. Forty-three compounds were identified in the EO of K. aegyptiaca, with a predominance of terpenoids (75.46%). Oxygenated compounds were the main class, with oxygenated sesquiterpenes attaining 40.42% of the EO total mass, while the oxygenated monoterpenes comprised 29.82%. The major compounds were cuminic aldehyde (21.99%), caryophyllene oxide (17.34%), hexahydrofarnesyl acetone (11.74%), ar-turmerone (8.51%), aromadendrene oxide (3.74%), and humulene epoxide (2.70%). According to the IC50 data, the K. aegyptiaca EO revealed considerable antioxidant activity, with IC50 values of 30.48 mg L−1 and 35.01 mg L−1 for DPPH and ABTS, respectively. In addition, the EO of K. aegyptiaca showed more substantial antibacterial activity against Gram-positive bacterial isolates compared to Gram-negative. Based on the minimum inhibitory concentration (MIC), the EO showed the highest activity against Escherichia coli and Bacillus cereus, with an MIC value of 0.031 mg mL−1. The present study showed, for the first time, that the EO of K. aegyptiaca has more oxygenated compounds with substantial antioxidant and antibacterial activities. This activity could be attributed to the effect of the main compounds, either singular or synergistic. Thus, further studies are recommended to characterize the major compounds, either alone or in combination as antioxidants or antimicrobial agents, and evaluate their biosafety.

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Isoscutellarein 8, 4′-Dimethyl ether glycosides as cytotoxic agents and chemotaxonomic markers in Kickxia aegyptiaca

Cited by 8 publications

References 33 publications

Effect of Dietary Supplement from Lymphoid Tissue of Chickens on Cell Viability

Effect of Dietary Supplement from Lymphoid Tissue of Chickens on Cell Viability

Novel 2‐oxo‐2‐phenylethoxy and benzyloxy diaryl urea hybrids as VEGFR‐2 inhibitors: Design, synthesis, and anticancer evaluation

Chemical Composition of Kickxia aegyptiaca Essential Oil and Its Potential Antioxidant and Antimicrobial Activities

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