Isotachophoresis as a candidate reference method in analytical chemistry

Lemmens, Aag; Everaerts, FM Frans; Janssen, Rtp; Hulsman, Jarj; Meijers, C.A.M.

doi:10.1016/s0021-9673(01)90495-9

Cited by 9 publications

(4 citation statements)

References 1 publication

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“…Another advantage is the ability to visually monitor the DNA band formation during the run as well as its amount because nucleic acid content in human blood may vary greatly. Finally, in view of the high flexibility of ITP (21,22), one may suppose that the method can be further improved and adapted to various new tasks.…”

Section: Resultsmentioning

confidence: 99%

Concentration and Isolation of DNA from Biological Fluids by Agarose Gel Isotachophoresis

Кондратова¹,

Serd'uk²,

Шелепов³

et al. 2005

BioTechniques

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Isotachophoresis is an electrophoretic method of separation of charged substances. The method is characterized by a discontinuous buffer system, constant velocity of separated molecules, and the distribution of separated components in the form of narrow concentrated bands located one right after another. As a rule, isotachophoresis is not used for the separation of nucleic acids because the mobility of polynucleotides in this system does not depend on their size. However, this circumstance proved to be very useful for the quantitative isolation of heterogeneous DNA fragments from biological fluids, for gene diagnostics of cancer in particular. The proposed method of agarose gel isotachophoresis of DNA has been used for the isolation of blood DNA and its successful PCR analysis.

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Section: Resultsmentioning

confidence: 99%

Concentration and Isolation of DNA from Biological Fluids by Agarose Gel Isotachophoresis

Кондратова¹,

Serd'uk²,

Шелепов³

et al. 2005

BioTechniques

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“…Importantly, it provides an opportunity to monitor the formation of DNA band in the course of electrophoresis and to assess its content, taking into account that the content of nucleic acids in human blood varies within a very broad range (if necessary, an additional aliquot of biological specimen may be added to well in gel). Finally, with regard for the high "plasticity" of isotachophoresis [4,5,15], it can be improved and adapted for various genodiagnostic problems.…”

Section: Counterflow Isotachophoresis As a Methods Of Concentration Anmentioning

confidence: 99%

Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

Кондратова¹,

Serdyuk²,

Шелепов³

et al. 2005

Dokl Biochem Biophys

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“…Isotachophoresis has also been examined as a candidate reference method. 39 Inductively coupled plasma mass spectrometry (ICP-MS) has not been extensively used for the determination of sodium in blood serum. The main challenge with the use of this technique is the relatively high instrumental background, which originates from sodium contamination, and which severely limits the dynamic range when using pulse counting ion detection.…”

Section: Introductionmentioning

confidence: 99%

Determination of sodium in blood serum by inductively coupled plasma mass spectrometry

Long

Vetter

2002

J. Anal. At. Spectrom.

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Owing to its importance as a serum electrolyte, sodium is determined routinely in clinical laboratories. In the United States, the accuracy of these measurements is assessed through a number of proficiency testing programs. Clinical measurements are supported by the National Institute of Standards and Technology through the production of serum matrix Standard Reference Materials 1 (909 and 956 series). For reference material certification, a gravimetric primary method has traditionally been used, but this method is time consuming. In this work, an alternative method has been developed in which sodium is determined by inductively coupled plasma mass spectrometry (ICP-MS) using a procedure, which is a cross between internal standardization and isotope dilution analysis. Diluted serum is spiked with 26 Mg enriched isotope and the isotope ratio 23 Na/ 26 Mg measured in analog detection mode. The ratios are standardized by measuring a sodium primary standard (SRM 919a) spiked with 26 Mg. As a relatively high concentration of sodium is measured, the sodium background from the ICP-MS instrument is comparatively small. The method has been successfully applied to the determination of sodium in SRM 909b (Human Serum), SRM 956a (Electrolytes in Frozen Human Serum) and serum pools from the College of American Pathologists.

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Isotachophoresis as a candidate reference method in analytical chemistry

Cited by 9 publications

References 1 publication

Concentration and Isolation of DNA from Biological Fluids by Agarose Gel Isotachophoresis

Concentration and Isolation of DNA from Biological Fluids by Agarose Gel Isotachophoresis

Counterflow Isotachophoresis As a Method of Concentration and Isolation of DNA from Biological Fluids

Determination of sodium in blood serum by inductively coupled plasma mass spectrometry

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