Isotopic Determination of Silicon by Mass Spectrometry in Plants and Soils Labeled with³⁰Si

Abstract

A method for isotopic determination of silicon by mass spectrometry in plants and soils labeled with 30 Si is reported. The development of this method is for use with studies involving the physiological process of absorption, transport, and redistribution of Si in the soil-plant system by use of the stable isotope 30 Si as a tracer. The procedure leads to SiF 4 formation, and the isotopic determination of Si was based on the measurements of the 28 SiF 3 þ , 29 SiF 3 þ , and 30 SiF 3 þ signals. Relative standard deviation of 30 Si abundance measurements (n ¼ 6) were lower than 0.1%, and the detection limit was 0.5 mg Si (dry mass).

Measuring production—dissolution rates of marine biogenic silica by 30Si‐isotope dilution using a high‐resolution sector field inductively coupled plasma mass spectrometer

“…It should permit more particularly the measurement of low rates on short time scales. Finally, we foresee potential applications to trace the origin and fate of Si in the course of complex processes such as reverse weathering in sediments (Michalopoulos and Aller 2004), formation of chemical complexes like hydroxyaluminisilicates (e.g., Doucet et al 2001), or Si uptake, transport, and precipitation within higher organisms such as plants (e.g., Opfergelt et al 2006, Carneiro et al 2008). These theoretical estimations take into account an average HR-SF-ICP-MS sensitivity of 1.10 6 /100 ppb (Table 1), the salinity of the MAGIC solution of 80 ± 8‰ per L seawater coprecipitated (average of 132 measurements), the salinity of alkaline digestion (~10‰), the salinity of solution purified by chromatography, which is less than 0.1‰ (8 measurements), the concentration factor of the alkaline digestion and the MAGIC solution (± 400 and 50 respectively), and the Si-requirement per ion-exchange column (2.0-3.6 µg Si).…”

Silício em plantas de feijão e arroz: absorção, transporte, redistribuição e tolerância ao cádmio

“…A fonte de silício utilizada foi o silicato de sódio, obtido a partir da fusão alcalina de 0,1 g de SiO 2 com 0,5 g de carbonato de sódio em mufla (CARNEIRO et al, 2008). O pH da solução nutritiva foi aferido e quando necessário adicionou-se HCl para acidificar ou NaOH para alcalinizar, objetivando o pH de 6,5 e condutividade elétrica de 2,5 mS cm -1 , os quais foram monitorados nas parcelas durante todo o período de condução do experimento.…”

Silicon speciation by hyphenated techniques for environmental, biological and industrial issues: A review

“…6). These techniques were mainly performed in environment (soil 193 and biogas 194,195 ) with variable sensitivity depending on the sample preparation (2 mg m À3 195 and 50 mg kg À1 193 ) and in polymer characterization (FT-ICR/MS and TOF/MS) usually as qualitative analysis for industrial applications.…”

“…46 After possible adsorption or deposition of silicon in the environment (section 1.2), a method for isotopic determination of 30 Si by MS in plants and soils was proposed with detection limit of 50 mg kg À1 for the soil samples. 193 MS is applied to have a better control of silicon compounds present in industrial applications such as semiconductors and packaging industries 196 and also in some processes. 197 For instance, in situ MS was carried out to characterize silicon compounds (tetraethoxysilane and hexamethyldisiloxane) enabling the production of protective layers for semiconductors.…”