Isotopic graphene–isolated-Au-nanocrystals with cellular Raman-silent signals for cancer cell pattern recognition

Zou, Yuxiu; Huang, Siqi; Liao, Yixin; Zhu, Xing; Chen, Yiqin; Chen, Long; Liu, Fang; Hu, Xiaoxiao; Tu, Haijun; Zhang, Liang; Liu, Zhangkun; Chen, Zhuo; Tan, Weihong

doi:10.1039/c7sc05442d

Cited by 56 publications

(50 citation statements)

References 69 publications

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“…Additionally, our chip-based exosome-PEARL SERS immunosensor offered more intuitionistic ways to discriminate cancer patients from healthy individuals by Raman imaging techniques with tremendous progress in spectral acquisition speed, detection sensitivity, spatial resolution, and penetration depth. 52 – 56 Briefly, the exosomes captured and SERS tag-labeled chip was fully scanned on its entire surface (7.2 × 1.8 cm 2 ) with a 200 μm step between each point. The anti-MIF-SERS chip containing eight pancreatic cancer samples and eight healthy samples was scanned in about 1 h. Then, the scanning results were analyzed by calculating the peak area of every point in the peak range (1045–1100 cm –1 ) and a color gradient was given to reveal the intensities.…”

Section: Resultsmentioning

confidence: 99%

An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer

et al. 2018

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Section: Resultsmentioning

confidence: 99%

An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer

et al. 2018

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“…All chromatograms were acquired and processed by the system software (Empower; Waters). To confirm the synthesis of Aptamer‐PEG‐Ce6, agarose gel electrophoresis was performed with 2% agarose gel . After the samples were loaded, a 1 × Tris‐borate‐EDTA (TBE) buffer as running solution was added into the outer buffer reservoir until the liquid level just covered the top surface of the gel.…”

Section: Methodsmentioning

confidence: 99%

Tumor‐Specific Aptamer‐Conjugated Polymeric Photosensitizer for Effective Endo‐Laparoscopic Photodynamic Therapy

Kim

Park

Kim

et al. 2019

Adv Funct Materials

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Recent advances in medical technology and endo-laparoscopic devices have enabled the treatment of gastrointestinal (GI) cancers to be minimally invasive through endo-laparoscopic photodynamic therapy (PDT). To achieve an efficient regional or endo-laparoscopic PDT, it is necessary to develop a highly target specific photosensitizer (PS) that can be easily treated to the lesion site with endo-laparoscopic device. Here, an ideal polymeric PS is demonstrated for effective endo-laparoscopic PDT. In the synthetic process, conventional PS (i.e., Chlorin e6, Ce6) is conjugated with an Aptamer (i.e., AS1411) targeting nucleolin (also called C23) overexpressed on the cancer cell membrane using a water-soluble polymeric linker (i.e., polyethylene glycol, PEG). The synthesized Aptamer-PEG-Ce6 could target nucleolinoverexpressing tumor cells efficiently and visualize the tumor tissues through optical and fluorescent imaging both in vitro and ex vivo, and effectively kills cancer cells under laser irradiation. Tumor staining with Aptamer-PEG-Ce6 is easily accomplished through endoscopic equipment within a few minutes. Furthermore, after laser irradiation, Aptamer-PEG-Ce6 is found to penetrate deeply into the tumor tissue and induce apoptosis of tumor cells. Taken together, the tumor-specific Aptamer-conjugated polymeric PS developed in this study has great potential as an ideal photomedicine for effective tumor treatment using endo-laparoscopic PDT.

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“…Besides one and two‐dimensional nanomaterials, as shown in Figure c, have been employed for cellular imaging. Application of GIANs conjugated with aptamers specific to cell membrane receptors including nucleolin, mucin, and epithelial cell adhesion molecule has been tested . Unlike Raman reporter‐labelled SERS metal and core‐shell nanoparticles, D, 2D, and G bands of GO coating at particular wavenumber are used as reference similar to that of Raman reporters.…”

Section: In Vitro Detection Of Cells/tissuesmentioning

confidence: 99%

Advances in surface‐enhanced Raman spectroscopy for cancer diagnosis and staging

Chakraborty

Ghosh

Barui

2019

J Raman Spectroscopy

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Surface‐enhanced Raman spectroscopy (SERS) is rapidly emerging as a bioanalytical tool for cancer diagnosis and therapy. The SERS‐based molecular diagnostics have progressed from proof‐of‐concept studies towards analysis in animal models as well as for in vitro clinical diagnostics in the last decade. Recently, SERS has also been implemented in screening, diagnosis, and staging of clinical cancer samples. Moreover, in vivo SERS imaging has been implemented for mapping the extent of tumor growth and metastasis; SERS nanoparticles have also enabled image‐guided therapies strongly indicating SERS technology can significantly complement the practice of oncology. Despite the progress, widespread clinical translation of SERS nanoparticles is still challenging. Current SERS strategies in diagnostic oncology require further development and standardization to progress from bench‐top to point‐of‐care applications. The present review critically analyzes the current state of the art about various strategies for SERS‐based cancer detection and staging from cellular metabolites, exosomes, circulating tumor cells, extracellular fluids, and cancer cells.

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Isotopic graphene–isolated-Au-nanocrystals with cellular Raman-silent signals for cancer cell pattern recognition

Abstract: Isotopic graphene–isolated-Au-nanocrystal SERS tags with simple, low background and super-stable fingerprint spectra were developed for pattern recognition of cancer cells.

Cited by 56 publications

References 69 publications

An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer

An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer

Tumor‐Specific Aptamer‐Conjugated Polymeric Photosensitizer for Effective Endo‐Laparoscopic Photodynamic Therapy

Advances in surface‐enhanced Raman spectroscopy for cancer diagnosis and staging

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