Isotretinoin Oxidation and Electroanalysis in a Pharmaceutical Drug Using a Boron‐doped Diamond Electrode

Abstract

Isotretinoin redox behaviour, by voltammetry, at the cathodic pre‐treated boron doped diamond electrode by cyclic, differential pulse and square wave voltammetry, and by spectrophotometry in a wide pH range, was investigated. The isotretinoin oxidation was an irreversible, diffusion‐controlled process, occurred in a three‐step cascade mechanism, and the isotretinoin diffusion coefficient D13‐cis‐RA=7.35×10−6 cm2 s−1, was calculated. The UV‐Vis isotretinoin maximum absorption was pH‐dependent. The detection limits LOD=0.53 μM, by differential pulse voltammetry, and LOD=0.38 μM, by UV‐Vis spectroscopy, were obtained. The isotretinoin in the pharmaceutical drug Roacutan®, using differential pulse voltammetry, was determined.

Preparation a core‐shell lipid/polymer nanoparticle containing Isotretinoin drug with pH sensitive property: A response surface methodology study

“…Since pKa of chitosan is 6.5, it is positively charged in the acidic environment and therefore suitable for encapsulating negatively‐charged agents. Therefore, with pKa of 5 for Isotretinoin, 48 by increasing pH, the negative surface charge of Isotretinoin is increased, resulting in stronger electrostatic interactions with CS and eventually enhanced entrapment efficiency. However, with the increase of pH value, the positive surface charge of CS decreases, but the impact of negative surface charge of drug at higher pH is more highlighted.…”