Isozyme analysis of genetic variability and population structure of Lactuca L. germplasm

El‐Esawi, Mohamed A.; Mustafa, Abd El-Zaher M.A.; Badr, Salwa; Sammour, R. H.

doi:10.1016/j.bse.2016.11.003

Cited by 25 publications

(17 citation statements)

References 19 publications

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“…The first step in making use of genetic resources, for human interest, is the collection of genetic resources and assessment of genetic diversity at both intra and inter accessional/population levels. The assessment of genetic diversity is necessary to increase utilization of plant genetic resources in varietals improvement (Sammour et al, 2007;Bakshi and Konnert, 2011;Ambade et al, 2015;Indira et al, 2015;El-Esawi et al, 2017). The advent of the electrophoresis method provides an indirect method for genome probing by exposing structural variations in enzymes and other proteins (Sammour, 1999;Thakur et al, 2018;El-Esawi et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The assessment of genetic diversity is necessary to increase utilization of plant genetic resources in varietals improvement (Sammour et al, 2007;Bakshi and Konnert, 2011;Ambade et al, 2015;Indira et al, 2015;El-Esawi et al, 2017). The advent of the electrophoresis method provides an indirect method for genome probing by exposing structural variations in enzymes and other proteins (Sammour, 1999;Thakur et al, 2018;El-Esawi et al, 2017). Electrophoretic markers are independent of species morphology and physiology and offer significant advantages over morphological methods of variety and/or species identification, in that: they are rapid, relatively cheap, eliminate the need to grow plants to maturity and are largely unaffected by the growth environment.…”

Section: Introductionmentioning

confidence: 99%

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Isozyme analysis of genetic variability and population structure of Lathyrus sativus L. germplasm

Sammour

Mustafa

Elsheikh

et al. 2019

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This work aimed at exploring the genetic variability, population structure and relationships of Lathyrus sativus L. germplasm using isozyme analysis. The data of isozyme analysis revealed 12 putative polymorphic loci of a total 33 alleles, indicating that studied accessions express good allelic richness and had an apparent rate of allogamy. The mean average of the expected heterozygosity (0.483) was more than the mean average of the observed heterozygosity (0.449) suggesting an apparent rate of allogamy taking place in Lathyrus sativus L. The average of total heterozygosity (HT) and intra-accessional genetic diversity (HS) were 0.559 and 0.428 respectively indicating that majority of genetic diversity was intra-accessional. The low levels and non-significant of genetic diversity among accessions (DST = 0.190, X2 = 62.59, p = 0.029) were probably indicative of occurrence of several gene flows. The coefficient of gene differentiation (GST) showed significant variation, suggesting the heterogonous distribution of L. sativus accessions among different geographic regions. The mean average of FST was 0.327, suggesting the occurrence of random mating system for the studied accessions and reflecting adaptation to strong environmental dissimilarities. Cluster analysis based on isozyme data suggested that the environment had no influence on the genetic diversity and confirmed that Lathyrus sativus L. had a polyphyletic origin.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isozyme analysis of genetic variability and population structure of Lathyrus sativus L. germplasm

Sammour

Mustafa

Elsheikh

et al. 2019

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“…Nowadays articles using isoenzymes as main technique (Litkowiec et al, 2013;Mathiasen and Premoli, 2013;Harter et al;Medoukali et al, 2015;Chlumský et al, 2016;Kutlunina et al, 2016;Luo et al, 2016;El-Esawi et al, 2017;Konzen and Martins, 2017) as well in association with molecular markers (Streiff et al, 1998;De Woody et al, 2009;Ekart et al, 2016;Reichmann et al, 2017;Quipildor et al, 2018;) are still published worldwide on population genetic studies. Although molecular markers are more informative and currently used, in some cases isozymes is still helpful to screen population and individuals…”

Section: Isoenzyme Electrophoresismentioning

confidence: 99%

Gene Diversity in Grevillea Populations Introduced in Brazil and Its Implication on Management of Genetic Resources

et al. 2018

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-We describe isoenzymes variability in six populations of Grevillea robusta from a provenances and progenies test established in a randomized block design with five plants per replication in Southern Brazil. The population genetic structure was examined by using biochemical markers in 5-year old trees, specifically at MDH-3, PGM-2, DIA-2, PO-1, PO-2, SOD-1, and SKDH-1 loci. The northern provenances (Rathdowney and Woodenbong) showed a strong divergence in relation to the average of provenances when alleles per locus (Ap), allele richness (Rs), Nei's gene diversity (H), and inbreeding coefficient (f) were considered. Inbreeding in varying degrees was detected. The commercial control showed the highest inbreeding coefficient, (f = 0.4448), whereas the provenance average was f = 0.2306, possibly due to insufficient sampling of populations in their origin (Australia). Despite its restricted natural range, a positive correlation between genetic divergence and geographic distance among original populations was detected. Genetic distance and cluster analyses based on the Bayesian model revealed three distinct provenance groups: 1) Rathdowney-QLD and Woodenbong-QLD; 2) Paddy's Flat-NSW; and 3) Mann River-NSW, Boyd River-NSW and the commercial control (material used in Brazil). The grouping of the control to both Mann River-NSW and Boyd River-NSW provenances suggests that the northern provenances have the highest potential for genetic improvement of wood productivity in Brazil, due to their high genetic diversity and low inbreeding coefficient. RESUMO -A variabilidade isoenzimática para seis populações de Grevillea robusta, oriundas de um teste de procedências/progenies, implantado no delineamento em blocos casualizados com 5 plantas por parcela, no Sul do Brasil, é descrita. A estrutura genética da população foi analisada utilizando-se marcadores bioquímicos, aos 5 anos de idade, especificamente para os locos MDH-3, PGM-2, DIA-2, PO-1, PO-2, SOD-1, e SKDH-1. As procedências do norte de ocorrência natural (Rathdowney e Woodenbong) apresentaram divergência genética superior, em relação à média das progênies, considerando o número de alelos por locus, (Ap), a riqueza alélica (Rs), a diversidade genética de Nei (H), e o coeficiente de endogamia (f). A endogamia foi detectada em diversos graus. A testemunha comercial apresentou o maior coeficiente de endogamia, (f = 0,4448), comparativamente à média das procedências (f = 0,2306), possivelmente devido à insuficiente amostragem populacional na região de origem (Austrália). Apesar de sua ocorrência natural restrita, observou-se correlação positiva entre divergência genética e distância geográfica entre as populações originais. A distância genética e análise de cluster, baseada no modelo bayesiano, mostrou três grupos de procedências distintos: 1) Rathdowney-QLD e Woodenbong-QLD; 2) Paddy's Flat-NSW; e 3) Mann River-NSW, Boyd River-NSW e a testemunha comercial (material utilizado no Brasil). O agrupamento da testemunha com as procedências Mann River-NSW e Boyd River-NSW...

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“…In conclusion, morphological, cytological, biochemical, and molecular markers proved useful in assessing genetic diversity levels in different plant species [22][23][24][25][26][27][28][29][30].…”

Section: Microsatellites (Ssrs)mentioning

confidence: 93%

Introductory Chapter: Assessment and Conservation of Genetic Diversity in Plant Species

El‐Esawi¹

2019

Genetic Diversity in Plant Species - Characterization and Conservation

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Isozyme analysis of genetic variability and population structure of Lactuca L. germplasm

Cited by 25 publications

References 19 publications

Isozyme analysis of genetic variability and population structure of Lathyrus sativus L. germplasm

Isozyme analysis of genetic variability and population structure of Lathyrus sativus L. germplasm

Gene Diversity in Grevillea Populations Introduced in Brazil and Its Implication on Management of Genetic Resources

Introductory Chapter: Assessment and Conservation of Genetic Diversity in Plant Species

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