Isozyme Analysis of Philippine Banana Cultivars/Species

Espino, R.R.C.; Pascua, G.S.

doi:10.17660/actahortic.1992.321.18

Cited by 2 publications

(1 citation statement)

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“…In the present study, different isozyme namely acid phosphatase, catalase, esterase and peroxidase have been used to test the genetic fidelity of micropropagated plants and to score somaclonal variation to differentiate the banana genotypes differing in their doses of 'A' and 'B' genomes. Similarly, Espino and Pascua (1992) reported the utilization of three isozyme for differentiating A and B genomes as well as AAB and ABB cultivars from BB/BBB cultivars. Lebot et al (1993) studied isozyme variation among 563 accessions of Musa including diploid AA, BB, triploid AAA, AAB, ABB and some tetraploid ABBB clones to determine genetic relationship.…”

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Isozyme analysis based genetic fidelity assessment of micropropagated banana plants

Suman

Kumari

Sharma

et al. 2015

JANS

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Isozyme studies of micropropagated and mother plants of banana cvs. Matti, Ney Poovan, Kechulepa, Dwarf Cavendish, Malbhog, Champa, B.B. Battisa and FHIA-1 were done to test their genetic fidelity. The banding patterns as revealed by electrophoretic variations were evaluated with respect to isozymes of acid phosphatase, catalase, esterase and peroxidase as markers. The genetic fidelity of micropropagated plants and the relationship of the different cultivars were determined by dendrogram using numerical taxonomy and multivariate analysis system (NTSYS). A clustered dendrogram was prepared by unweighted pair group method using averages (UPGAMA) method. At 87% similarity, the micropropagated and mother plants were clustered in four groups reflecting their genomic constitution. Cvs. Matti (AA) and Dwarf Cavendish (AAA) with similar 'A' genome were categorized in Cluster I. Cluster II comprised of cvs. Ney Poovan (AB), B.B. Battisa (ABB) and FHIA-1(AAAB) with genomic constitution of both 'A' and 'B' type. Cvs. Champa (AAB) and Malbhog (AAB) with similar genome were grouped in Cluster III. Cluster IV contained the cv. Kechulepa (BB) having only 'B' genome. However, there was no somaclonal variation among the micropropagated plants and they showed 100% genetic similarity. Thus, the isozyme studies could be a reliable marker for testing the genetic fidelity of micropropagated plants and for evaluating the diversity among the banana germplasm.

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confidence: 98%