Ecotourism is a type of tourism whose main motivation is to protect the integrity of the ecosystem with a responsible tourism scientist and should be handled under the umbrella of sustainability. Considering the principles of protecting nature and not destroying nature in ecotourism, natural areas are accepted as the home of all of us and are envisaged as a tool for both conservation and sustainable development. Isparta province is a rich destination in terms of ecotourism activities with its unspoiled nature and rich ecology. Various studies have been carried out on ecotourism in the region and projects related to ecotourism are supported. In the study, firstly, the ecotourism routes in the destination were discussed, and a focus group interview was conducted to determine the economic, political, socio-cultural, technological and ecological dimensions of ecotourism in the destination with PESTE analysis, which is a strategic and external environment analysis. The opportunities and threats of ecotourism activities in the destination for the region were examined. It is thought that the study will contribute to the field of tourism and ecotourism activities and projects to be carried out in the region.