Issue Highlights—January 2013

Lanza, Francesco

doi:10.1002/cyto.b.21054

Cited by 6 publications

(7 citation statements)

References 38 publications

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“…Flow cytometry immunophenotyping (FCI) of paucicellular specimens such as cerebrospinal fluid (CSF) has historically been challenging due to the primary lack of sufficient cells for adequate testing purposes as a result of rapid cell loss post‐specimen collection. Several flow cytometric protocols designed to increase cellular yield have been reported, including immediate stabilization of cells in Transfix™ reagent, RPMI 1640 cell culture media with fetal calf serum (FCS), and phosphate buffered saline (PBS) with FCS at the time of specimen collection (). Transfix, however, requires exact volume to reagent ratios and may alter intensity of surface antigen staining.…”

Section: Introductionmentioning

confidence: 99%

Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing

Greig

Stetler-Stevenson

Lea

2013

Cytometry Part B Clinical

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Section: Introductionmentioning

confidence: 99%

Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing

Greig

Stetler-Stevenson

Lea

2013

Cytometry Part B Clinical

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“…Bone marrow shows variable infiltration by plasma cells, indistinguishable from that seen in MM. Plasma cell phenotype and cytogenetic abnormalities are the same as those observed in myeloma 7,8 , though markers of bad prognosis, such as p53 deletions or hypodiploidy, are more frequent in PCL 5,6 . Phenotypically, plasma cells are CD138+/CD38+/CD45-or weakly positive.…”

Section: Introductionmentioning

confidence: 87%

A case of CD138-/CD19+/CD4+ IgD plasma cell leukemia

et al. 2014

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Background: Plasma cell leukemia (PCL) is an uncommon and aggressive disease caused by the clonal proliferation of atypical plasma cells with phenotypical abnormalities similar to those seen in multiple myeloma (MM), although at different rates. Here we report a case of IgD PCL with a very unusual CD138-/CD19+/CD4+ phenotype Methods: Peripheral blood and bone marrow samples from a 37 year old patient afflicted by an aggressive plasma cell dyscrasia were examined and analyzed by conventional morphology, flow cytometry and immunohistochemistry. Results: Analysis of peripheral blood fulfilled criteria for PCL (more than 20% and more than 2x10e9 cells/L). However, flow cytometry and immunohistochemistry phenotyping revealed that the cells were CD138-/CD38+/CD19+/CD4+/CD56-/CD117- Conclusion: PCL is diagnosed on peripheral blood smear. Immunophenotyping is a tool that can be helpful in diagnosing difficult cases but its atypical findings should not prevent the appropriate PCL diagnosis in clinically and morphologically unquestionable cases. © 2014 Clinical Cytometry Society.

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“…Please cite this article as an 'Accepted Article', doi: 10.1002/cytob.21172 cytometry for the identification of MDS patients possibly responding to novel cytostatic drugs. Since, it is well known that in high risk MDS patients treatment with azacitidine is associated with prolonged overall survival in patients who respond to therapy, the use of multiparametric flow cytometry may represent a new tool for refining treatment modality in MDS patients (6)(7)(8)(9)(10)(11). In particular, by using the flow cytometric scoring system (FCSS), the presence of myeloid progenitors having an aberrant immunophenotype was significantly associated with lack of response to azacytidine therapy.…”

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confidence: 99%

CYTOB-83B3-highlights

Lanza

2014

Cytometry

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The review article by Mauland et al provided evidence that the DNA content assessment zmay be of clinical value in endometrial cancer (EC) (1). As many of us may recall, DNA ploidy in EC was introduced as a prognostic marker 30 years ago (2). Despite the accumulating evidence that tumor aneuploidy is associated with poorer clinical outcome, this parameter was not incorporated into daily clinical practice for a number of reasons, mainly due to the lack of a routine diagnostic test validating its clinical applicability in EC. Looking at literature data in this field, the majority of the studies demonstrated highly significant correlation between DNA aneuploidy and survival in both univariate and multivariate analysis. Unfortunately, few studies have focused on DNA ploidy as a prognostic marker in a prospective setting. In conclusion, based on the reviewed literature, authors concluded that DNA ploidy analysis in hysterectomy specimen is superior to analysis in curettage material and may predict lymph node metastasis. However, the clinical significance of tumor aneuploidy in this setting may be hampered by the methodology used (flow cytometry, image cytometry and laser scanning cytometry), thus limiting the clinical applicability of this analysis.

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Issue Highlights—January 2013

Cited by 6 publications

References 38 publications

Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing

Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing

A case of CD138-/CD19+/CD4+ IgD plasma cell leukemia

CYTOB-83B3-highlights

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