In this quantitative review, we investigate the degree to which landscape ecology studies that use spatial data address spatial uncertainty when conducting analyses. We identify three broad categories of spatial uncertainty that are important in determining the characterisation of landscape pattern and affect the outcome of analysis in landscape ecology: i) classification scheme uncertainty, ii) spatial scale and iii) classification error. The second category, spatial scale, was further subdivided into five scale dependent factors i) pixel size ii) minimum mappable unit, iii) smoothing, iv) thematic resolution and v) extent. We reviewed all articles published in the journal Landscape ecology in 2007 and recorded how spatial data was used and whether spatial uncertainty was addressed or reported in ecological analyses. This review found that spatial uncertainty was rarely addressed and/or reported. Most articles used the default pixel size of the sensor. Only 23% of articles addressed one or more scale dependent factors and 47% reported one or more as issues. A single study of the 59 investigated the effect of classification accuracy on ecological analyses. We demonstrate that spatial uncertainty is not being addressed as standard practice in analyses in landscape ecology, and then describe methods to test for spatial uncertainty and potential solutions that can be developed in the future.