ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells

Morandi, Evi M.; Verstappen, Ralph; Zwierzina, Marit; Geley, Stephan; Pierer, Gerhard; Ploner, Christian

doi:10.1038/srep28889

Cited by 97 publications

(82 citation statements)

References 61 publications

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“…Previous studies have shown that downregulation of NANOG and Oct4 expression can reduce cell pluripotency (Boyer et al, ; Chambers et al, ), and that knocking out Oct4 can impair blastocyst development (Simmet et al, ). Similarly, NANOG and ITGA5 not only help to maintain embryonic stem cell pluripotency but also promote cell proliferation (Koshida et al, ; Morandi et al, ). Our results showed that KAE‐treated aged oocytes present remarkably higher NANOG , ITGA5 , and Oct4 mRNA expression levels than untreated aged oocytes.…”

Section: Discussionmentioning

confidence: 99%

Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes

Yao

Jiang

et al. 2019

Animal Science Journal

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Kaempferol (KAE) is a natural flavonoid present in different plant species and exhibits anti‐inflammatory, antioxidant, and anticancer therapeutic properties. In the present study, we investigated the influence and underlying mechanisms of KAE supplementation on porcine oocytes during in vitro aging. The results show that KAE treatment can alleviate the aging‐related reduction of developmental competence. We observed that the blastocyst production rate in aged oocytes treated with 0.1 μM KAE was significantly higher than in untreated aging oocytes (36.78 ± 0.86% vs. 27.55 ± 2.60%, respectively, p < .05). The KAE‐treated aging oocytes had significantly reduced levels of reactive oxygen species (p < .05). Furthermore, the mRNA levels of the embryonic pluripotency‐related genes Oct4, NANOG, and ITGA5 were significantly increased in blastocysts derived from KAE‐treated oocytes (p < .05). During excessive oocyte culture, KAE treatment maintained the mitochondrial membrane potential and reduced apoptosis; however, this was not observed in untreated aging oocytes. In conclusion, our results suggest that KAE treatment can alleviate the aging of porcine oocytes by reducing oxidative stress and improving mitochondrial function.

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Section: Discussionmentioning

confidence: 99%

Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes

Yao

Jiang

et al. 2019

Animal Science Journal

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“…The EMT involves the conversion of epithelial cells to migratory and invasive cells, and the activation of the EMT is closely associated with the motility and invasiveness of GC cells . FZD7 , CDH2 , TGFB1 , SPARC , ITGA5, and ZWB1 are associated with the EMT and malignant cell function, indicating that SUSD2 may play a role in the WNT pathway and the EMT . Therefore, SUSD2 may serve as a therapeutic target for inhibiting inappropriate WNT signaling or induction of the EMT.…”

Section: Discussionmentioning

confidence: 99%

“…24,25 FZD7, CDH2, TGFB1, SPARC, ITGA5, and ZWB1 are associated with the EMT and malignant cell function, indicating that SUSD2 may play a role in the WNT pathway and the EMT. 23,[26][27][28][29][30] Therefore, SUSD2 may serve as a therapeutic target for inhibiting inappropriate WNT signaling or induction of the EMT.…”

Section: Discussionmentioning

confidence: 99%

Expression of sushi domain containing two reflects the malignant potential of gastric cancer

et al. 2018

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Hepatic recurrence of gastric cancer (GC) is uncontrollable. Discovery of causative oncogenes and the development of sensitive biomarkers to predict hepatic recurrence are required to improve patients’ outcomes. In this study, recurrence pattern‐specific transcriptome analysis of 57 749 genes was conducted to identify mRNAs specifically associated with hepatic metastasis of patients with stage III GC who underwent curative resection. GC cell lines were subjected to mRNA expression analysis, PCR array analysis, and siRNA‐mediated knockdown. The expression levels of primary cancer tissues from 154 patients with resectable GC were determined and correlated with clinicopathological variables. Among 21 genes significantly overexpressed specifically in patients with hepatic recurrence, Sushi domain containing 2 (SUSD2) was selected as a promising target. PCR array analysis revealed that SUSD2 mRNA levels positively correlated with those of FZD7, CDH2, TGFB1, SPARC, ITGA5, and ZEB1. Functional analysis revealed that knockdown of SUSD2 significantly reduced the proliferation, migration, and invasiveness GC cell lines. Patients with high SUSD2 expression were more likely to experience shorter disease‐free and overall survival. Analysis of the relation between disease recurrence pattern and SUSD2 levels revealed that significantly more patients with hepatic metastases expressed higher levels of SUSD2 mRNA. The cumulative incidence of hepatic recurrence was greater in patients with high SUSD2 expression. In conclusion, SUSD2 likely contributes to the malignant potential of GC and may serve as a novel biomarker that predicts hepatic recurrence after curative resection.

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“…ITGA5 was strongly expressed in undifferentiated cells, while downregulated in MAs. The strongest effect of ITGA5/ITGAV knockdown was an increase in differentiation and, on the contrary, transgenic ITGA5/ITGAV expression determined adipogenesis inhibiting effects (Morandi et al, ).…”

Section: Introductionmentioning

confidence: 99%

Key‐genes regulating the liposecretion process of mature adipocytes

Maurizi

Petäistö

Maurizi³

et al. 2017

Journal Cellular Physiology

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White mature adipocytes (MAs) are plastic cells able to reversibly transdifferentiate toward fibroblast-like cells maintaining stem cell gene signatures. The main morphologic aspect of this transdifferentiation process, called liposecretion, is the secretion of large lipid droplets and the development of organelles necessary for exocrine secretion. There is a considerable interest in the adipocyte plastic properties involving liposecretion process, but the molecular details are incompletely explored. This review analyzes the gene expression of MAs isolated from human subcutaneous fat tissue with respect to bone marrow (BM)-derived mesenchymal stem cells (MSC) focusing on gene regulatory pathways involved into cellular morphology changes, cellular proliferation and transports of molecules through the membrane, suggesting potential ways to guide liposecretion. In particular, Wnt, MAPK/ERK, and AKT pathways were accurately described, studying up- and down-stream molecules involved. Moreover, adipogenic extra- and intra-cellular interactions were analyzed studying the role of CDH2, CDH11, ITGA5, E-Syt1, PAI-1, IGF1, and INHBB genes. Additionally, PLIN1 and PLIN2 could be key-genes of liposecretion process regulating molecules transport through the membrane. All together data demonstrated that liposecretion is regulated through a complex molecular networks that are able to respond to microenvironment signals, cytokines, and growth factors. Autocrine as well as external signaling molecules might activate liposecretion affecting adipocytes physiology.

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ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells

Cited by 97 publications

References 61 publications

Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes

Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes

Expression of sushi domain containing two reflects the malignant potential of gastric cancer

Key‐genes regulating the liposecretion process of mature adipocytes

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