iTRAQ-based proteomics analysis of Bacillus pumilus responses to acid stress and quorum sensing in a vitamin C fermentation system

Zhang, Qian; Lyu, Shuxia

doi:10.3389/fmicb.2023.1131000

Cited by 5 publications

(2 citation statements)

References 48 publications

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“…Following the maturation and release of spores, a large number of active substances, such as purines, amino acids, fatty acids, and vitamin B 1 , are released into the medium, which can promote the growth of K. vulgare and 2-KGA production. Lyu et al 52,53 found that addition of the supernatant of the associated bacteria in the middle and late stages of the fermentation period, that is, the period of spore formation and release, can greatly promote the accumulation of 2-KGA. A comparison of the growth of B. endophyticus ST-1 in single or mixed culture with K. vulgare 25-B-1 by Lyu et al 54,55 revealed a shortened growth period of K. vulgare 25-B-1 and faster growth of the associated bacteria in the mixed-culture system.…”

Section: The Interaction Between Associated Bacteria and K Vulgarementioning

confidence: 99%

“…vulgare and 2-KGA production. Lyu et al , found that addition of the supernatant of the associated bacteria in the middle and late stages of the fermentation period, that is, the period of spore formation and release, can greatly promote the accumulation of 2-KGA. A comparison of the growth of B.…”

Section: Characteristics Of Key Strains Employed In the Classic Two-s...mentioning

confidence: 99%

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Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Liu,

Chen

et al. 2024

J. Agric. Food Chem.

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Vitamin C, also known as ascorbic acid, is an essential vitamin that cannot be synthesized by the human body and must be acquired through our diet. At present, the precursor of vitamin C, 2-keto-L-gulonic acid (2-KGA), is typically produced via a two-step fermentation process utilizing three bacterial strains. The second step of this traditional two-step fermentation method involves mixed-culture fermentation employing 2-KGA-producing bacteria (Ketogulonicigenium vulgare) along with associated bacteria. Because K. vulgare has defects in various metabolic pathways, associated bacteria are needed to provide key substances to promote K. vulgare growth and 2-KGA production. Unlike previous reviews where the main focus was the interaction between associated bacteria and K. vulgare, this Review presents the latest scientific research from the perspective of the metabolic pathways associated with 2-KGA production by K. vulgare and the mechanism underlying the interaction between K. vulgare and the associated bacteria. In addition, the dehydrogenases that are responsible for 2-KGA production, the 2-KGA synthesis pathway, strategies for simplifying 2-KGA production via a one-step fermentation route, and, finally, future prospects and research goals in vitamin C production are also presented.

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Section: The Interaction Between Associated Bacteria and K Vulgarementioning

confidence: 99%

Section: Characteristics Of Key Strains Employed In the Classic Two-s...mentioning

confidence: 99%

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Liu,

Chen

et al. 2024

J. Agric. Food Chem.

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Rhodotorula mucilaginosa A8, a potential helper strain in a vitamin C microbial fermentation process

Zhang,

Liao,

Lyu

2024

J Basic Microbiol

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In the vitamin C microbial fermentation system, oxidative stress limits the growth and 2‐keto‐l‐gulonic acid (2‐KLG, the precursor of vitamin C) production of Ketogulonicigenium vulgare. Most Bacillus strains, as helper strains, have been reported to release key biomolecules to reduce oxidative stress and promote the growth and 2‐KLG production of K. vulgare. To understand the specific mechanism by which the helper strain and K. vulgare interact to reduce oxidative stress, a novel helper strain, Rhodotorula mucilaginosa A8, was used to construct a consortium in the co‐culture fermentation system. Based on the activities of the antioxidant enzymes and quantitative polymerase chain reaction (qPCR) analysis, R. mucilaginosa A8 could reduce oxidative stress and increase 2‐KLG production in K. vulgare by upregulating antioxidant enzyme activities and related gene‐expression levels. In addition, the carotenoids of R. mucilaginosa promoted 2‐KLG production in K. vulgare. Coculture of R. mucilaginosa with K. vulgare increased the yield of carotenoids. This study suggested that helper strains with the ability to reduce oxidative stress in K. vulgare would likely act as potential helper strains for facilitating 2‐KLG biosynthesis. This work could provide a theoretical basis for the search for potential helper strains for vitamin C microbial fermentation and for the construction of synthetic microbial communities to produce valuable products.

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2-Keto-l-gulonic acid inhibits the growth of Bacillus pumilus and Ketogulonicigenium vulgare

Zhang

Lyu

2023

World J Microbiol Biotechnol

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iTRAQ-based proteomics analysis of Bacillus pumilus responses to acid stress and quorum sensing in a vitamin C fermentation system

Cited by 5 publications

References 48 publications

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Rhodotorula mucilaginosa A8, a potential helper strain in a vitamin C microbial fermentation process

2-Keto-l-gulonic acid inhibits the growth of Bacillus pumilus and Ketogulonicigenium vulgare

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