IVM is an alternative for patients with PCO after failed conventional IVF attempt

Gülekli, Bülent; Kovalı, Müge; Aydıner, Fulya; Doğan, Şule; Doğan, Seda Sultan

doi:10.1007/s10815-011-9591-z

Cited by 9 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cryopreservation of GV oocytes by vitrification offers attractive perspectives [ 59 ], since the resulting GV oocytes represent an important pool of germ cells for women undergoing unstimulated IVM cycles or at risk of infertility due to ovarian hyperstimulation syndrome or polycystic ovarian syndrome, particularly in those who are poor responders to gonadotropin stimulation [ 60 ] or in the case of cancer [ 61 ]. Rescue of immature oocytes can also be performed in an IVF cycle when a conventional protocol fails to produce mature oocytes.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of immature and mature human oocytes after cryotop vitrification

Palmerini

Antinori²,

Maione

et al. 2014

Journal of Reproduction and Development

View full text Add to dashboard Cite

In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic organization of fresh and vitrified oocytes did not show significant changes. GVv oocytes showed significant ultrastructural alterations of the microvilli in 40% of the samples; small vacuoles and occasional large/isolated vacuoles were abnormally present in the ooplasm periphery of 50% of samples. The ultrastructure of nuclei and mitochondria-vesicle (MV) complexes, as well as the distribution and characteristics of cortical granules (CGs), were comparable with those of GVc oocytes. MIIv oocytes showed an abnormal ultrastructure of microvilli in 30% of the samples and isolated large vacuoles in 70% of the samples. MV complexes were normal, but mitochondria-smooth endoplasmic reticulum aggregates appeared to be of reduced size. CGs were normally located under the oolemma but presented abnormalities in distribution and matrix electron density. In conclusion, Cryotop vitrification preserved main oocyte characteristics in the GV and MII stages, even if peculiar ultrastructural alterations appeared in both stages. This study also showed that the GV stage appears more suitable for vitrification than the MII stage, as indicated by the good ultrastructural preservation of important structures that are present only in immature oocytes, like the nucleus and migrating CGs.

show abstract

Section: Discussionmentioning

confidence: 99%

Ultrastructure of immature and mature human oocytes after cryotop vitrification

Palmerini

Antinori²,

Maione

et al. 2014

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

“…Gulekli et al ( 46 ) studied 23 women who failed in conventional IVF and were transferred to IVM without ovarian stimulation. Only one pregnancy was obtained and that did not continue to birth, and the authors concluded that IVM might be a useful tool for failed conventional IVF ( 46 ) . As an uncommon indication, IVM may be an important optional choice in cases with oocyte maturation abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Oocyte in vitro maturation: A sytematic review

Hatırnaz¹,

Ata

Hatırnaz³

et al. 2018

tjod

View full text Add to dashboard Cite

In vitro maturation (IVM) is one of the most controversial aspects of assisted reproductive technology. Although it has been studied extensively, it is still not a conventional treatment option and is accepted as an alternative treatment. However, studies have shown that IVM can be used in almost all areas where in vitro fertilization (IVF) is used and it has a strong place in fertility protection and Ovarian Hyperstimulation syndrome management. The aim of this systematic review was to address all aspects of the current knowledge of IVM treatment together with the evolution of IVM and IVF.

show abstract

“…However, accumulating data from articles supporting IVM because it is safe, cost-effective, simple, repeatable, flexible, and patient friendly in nature, without the risk of OHSS, it serves as a good treatment option. As such, IVF specialists need to be encouraged to add IVM to their clinical practice instead of neglecting it ( 18 , 19 , 20 , 21 ) . There are few publications regarding rescue IVM in the literature.…”

Section: Discussionmentioning

confidence: 99%

The use of in vitro maturation in stimulated antagonist in vitro fertilization cycles of normo-hyperresponder women due to arrested follicular development: A rescue procedure

Hatırnaz¹,

Akarsu

Hatırnaz³

et al. 2018

tjod

View full text Add to dashboard Cite

Objective: To evaluate the impact of rescue in vitro maturation (IVM) on the clinical outcomes of women with arrested follicular development in stimulated in vitro fertilization (IVF) cycles.Materials and Methods:This is a retrospective review of 13 patients who were evaluated as normo-hyperresponders for ovarian stimulation. The main outcome measure was the clinical pregnancy and livebirth rates. The purpose of gonadotropin stimulation in patients undergoing IVF is to retrieve multiple oocytes by avoiding multifetal gestation and Ovarian Hyperstimulation syndrome (OHSS). The ovarian response to stimulation ranges from poor response to OHSS, which is related to the follicular number and the dose of the gonadotropins used. However, in some cycles of normo-hyperresponder women, follicular development decelerates or ceases. Close follow-up in a daily manner and increasing the dose of gonadotropins did not change the follicular arrest. This clinical situation has two edges; one is cycle cancellation, which has undesired psychological outcomes for women and the IVF team, and second one is the prolongation of the IVF cycle. For such circumstances, IVM may be a valuable option. Stimulated IVF cycles were converted to IVM as a rescue IVM procedure following detailed informed consent of the women who were close to cycle cancellation.Results:Thirteen 13 IVM cycles and their clinical outcomes are presented. Six women achieved pregnancies, but only 4 delivered 5 healthy live born. The other two women had biochemical loss during follow-up.Conclusion:Based on the data obtained, it can be concluded that gonadotropin-stimulated cycles with follicular arrest at the edge of cancellation can be shifted to rescue IVM procedures with reasonable clinical outcomes.

show abstract

IVM is an alternative for patients with PCO after failed conventional IVF attempt

Cited by 9 publications

References 30 publications

Ultrastructure of immature and mature human oocytes after cryotop vitrification

Ultrastructure of immature and mature human oocytes after cryotop vitrification

Oocyte in vitro maturation: A sytematic review

The use of in vitro maturation in stimulated antagonist in vitro fertilization cycles of normo-hyperresponder women due to arrested follicular development: A rescue procedure

Contact Info

Product

Resources

About