Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis

Palmarini, Massimo; Holland, Martin J.; Cousens, Chris; Dalziel, Robert; Sharp, J. M.

doi:10.1099/0022-1317-77-12-2991

Cited by 104 publications

(114 citation statements)

References 38 publications

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“…Considering that the virus reservoir in an infected animals seems to be the lymphoreticular system (Palmarini et al, 1996b;González et al, 2001;Caporale et al, 2005) and in agreement with other authors that the lamb infection may occurs perinatally or in the first few months of life (Caporale et al, 2005) maternal routes may be important in the JSRV transmission. Milk transmission is an important route of spread of ovine lentiviruses (Pepin et al, 1998) and for many retroviruses either causing immunodeficiencies (Friendland and Klein, 1987) or tumours (Buffet et al, 1969;Coffin, 1996).…”

supporting

confidence: 64%

“…Free virus in the plasma or produced in the mammary glands is contained in milk and infects young individuals. In spite JSRV transcripts have been found in several lymphoid organs, it has not been found in the plasma using very specific and sensitive PCR procedures in other studies (Palmarini et al, 1996b). Furthermore, proviral DNA was not found in mammary glands either in OPA clinically affected sheep or in pre-clinical status (González and Ruiz, 1995) and mammary gland tissues obtained from OPA affected animals were negative by immunohistochemistry using a rabbit anti-SU-JSRV (author's unpublished results).…”

mentioning

confidence: 99%

“…As the tumour cells are secretory, a considerable amount of fluid can be generated and accumulated in the respiratory tract of the affected animals and the most typical feature of clinical OPA is the discharge of a whitish foamy fluid from the nostrils (De las Heras et al, 2003). The presence of the JSRV can be confirmed in the lung fluid or tumours by immunoblotting (Sharp and Herring, 1983), immunohistochemistry Palmarini et al, 1995;Salvatori et al, 2004), ELISA (Palmarini et al, 1995) and PCR (Bai et al, 1996;Palmarini et al, 1996b). In OPA affected sheep, JSRV is also detectable in cells of the lymphoreticular system, employing a highly sensitive PCR assay (Palmarini et al, 1996b;Holland et al, 1999) or immunohistochemistry (Holland et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of the JSRV can be confirmed in the lung fluid or tumours by immunoblotting (Sharp and Herring, 1983), immunohistochemistry Palmarini et al, 1995;Salvatori et al, 2004), ELISA (Palmarini et al, 1995) and PCR (Bai et al, 1996;Palmarini et al, 1996b). In OPA affected sheep, JSRV is also detectable in cells of the lymphoreticular system, employing a highly sensitive PCR assay (Palmarini et al, 1996b;Holland et al, 1999) or immunohistochemistry (Holland et al, 1999). In spite of this, it is more difficult to identify animals during pre-clinical period due to lack of detectable circulating JSRV-specific antibodies (Sharp and Herring, 1983;Ortín et al, 1998;Summers et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Colostrum and milk can transmit jaagsiekte retrovirus to lambs

Grego

Meneghi

Álvarez

et al. 2008

Veterinary Microbiology

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Ovine pulmonary adenocarcinoma (OPA) is a contagious disease caused by jaagsiekte sheep retrovirus (JSRV). In the three studies performed, we have obtained data of the importance of colostrum/milk (C/M) in the transmission of JSRV. In the first study, a group of sheep from a flock with a long history of OPA, samples from colostrum and peripheral blood leucocytes (PBLs) were collected. Two specific PCRs (U3-LTR and env of the JSRV) were carried out. Using U3PCR 8/34 sheep were positive in colostrum whereas with envPCR 7/34 were positive. From these animals only one was positive with U3PCR in the PBLs. Evidence of the transmission of JSRV infection by C/M was obtained in two more separate studies. In the second study, PBLs from five lambs from JSRV+ ewes and two from JSRV-ewes were tested by the U3PCR. They were fed C/M by their mothers during 3 months and slaughtered 7 months after birth. Three out of five lambs from the JSRV+ sheep become PBL positive at 3-4 months old and the other two were also positive at 4-6 months of age. One lamb of the JSRV-sheep became also PBL positive at an age of 3 months. In the third study, a group of lambs from JSRV negative mothers were fed with C/M from JSRV+ sheep and housed in separate unit. For comparison, another group of the same origin and maintained in another different unit, were fed with C/M containing a JSRV virus preparation. All lambs were blood sampled monthly and JSRV infection was detected as early as 15 days and several times onwards in both groups. Control groups fed with C/M from JSRV free flock and JSRV blood test negative sheep were always negative. Together these results indicate that suckling is an important natural transmission route for JSRV. #

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supporting

confidence: 64%

mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Colostrum and milk can transmit jaagsiekte retrovirus to lambs

Grego

Meneghi

Álvarez

et al. 2008

Veterinary Microbiology

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“…These viruses are closely related to each other and to sheep endogenous retroviral sequences (ovERVs) at the nucleic acid level (Hecht et al, 1994;Cousens et al, 1996Cousens et al, , 1999. The close homology between JSRV, ENTV and ovERVs was a problem for the development of specific detection techniques for these viruses, but this was resolved by the identification of small areas of sequence that differ between them (Bai et al, 1996;Palmarini et al, 1996;Cousens et al, 1996Cousens et al, , 1999. The suggestion that ENTV of sheep and ENTV of goats are distinct viruses (De las Heras et al, 2003) led us to undertake the complete sequencing of a goat ENTV isolate, named ENTV-2, using samples from a single ENA-affected goat.…”

Section: Introductionmentioning

confidence: 99%

Characterization of enzootic nasal tumour virus of goats: complete sequence and tissue distribution

Ortı́n

Cousens

Minguijón

et al. 2003

Journal of General Virology

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The complete genome sequence of a new isolate of enzootic nasal tumour virus (ENTV-2), associated with enzootic nasal adenocarcinoma (ENA) of goats, was determined. The genome exhibits a genetic organization characteristic of b-retroviruses. ENTV-2 is closely related to the retrovirus (ENTV-1) associated with enzootic adenocarcinoma of sheep, and to jaagsiekte retrovirus. The main sequence differences between these viruses reside in orfX, the U3 LTR, two small regions in gag and the transmembrane (TM) region of env. Sequence analysis of the TM region of env from several sheep and goats naturally affected by ENA suggested that ENTV-1 and ENTV-2 are distinct viruses rather than geographical variants. Although both viruses transform secretory epithelial cells of the ethmoid turbinate, the study of their tissue distribution using specific PCRs showed that ENTV-2 establishes a disseminated lymphoid infection whereas ENTV-1 is mainly confined to the tumour.

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Sequence Comparison of JSRV with Endogenous Proviruses: Envelope Genotypes and a Novel ORF with Similarity to a G-Protein-Coupled Receptor

et al. 1999

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Ovine pulmonary carcinoma, a contagious lung cancer of sheep, is caused by the oncogenic jaagsiekte sheep retrovirus (JSRV) that is closely related to a family of endogenous sheep retroviral sequences (ESRVs). By using exogenous virus-specific U3 oligonucleotide primers, the entire JSRV proviral genome or its 3' part was amplified from tumor DNA. Analysis of these proviral sequences revealed a novel open reading frame (ORF) within the pol coding region, designated ORF X, which was well conserved in ESRV and JSRV sequences. Deduced amino acids of ORF X showed similarity to a portion of the mammalian adenosine receptor subtype 3, a member of the G-protein-coupled receptor family. Comparison of deduced env amino acids of six JSRV strains from three continents identified 15 residues that defined two distinct genotypes of JSRVs. Sequence analysis identified two highly variable regions between JSRV and ESRV in the transmembrane domain of env (TM) and the 3' unique sequence (U3) of the long terminal repeat, from which JSRV-specific DNA probes were derived. By using these DNA probes in Southern hybridization, for the first time we successfully identified JSRV proviral sequences in tumor genomic DNA in the presence of multiple ESRV loci, validating the use of exogenous virus-specific DNA probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences.

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Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis

Cited by 104 publications

References 38 publications

Colostrum and milk can transmit jaagsiekte retrovirus to lambs

Colostrum and milk can transmit jaagsiekte retrovirus to lambs

Characterization of enzootic nasal tumour virus of goats: complete sequence and tissue distribution

Sequence Comparison of JSRV with Endogenous Proviruses: Envelope Genotypes and a Novel ORF with Similarity to a G-Protein-Coupled Receptor

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