Jacalin Has Chemopreventive Effects on Colon Cancer Development

Abstract

Colorectal cancer, which is one of the most common causes of cancer-related deaths worldwide, has a slow natural history that provides a great opportunity for prevention strategies. Plant-derived natural products have received considerable attention because of their inherent colorectal cancer chemopreventive effects. The plant lectin jacalin specifically recognizes the tumor-associated Thomsen-Friedenreich antigen and has antiproliferative effects on human colon cancer cells, highlighting its potential antitumor activity. To evaluate jacalin's potential application in colorectal cancer chemoprevention, we studied its effects on the early stages of carcinogenesis. Balb/c mice were given 4 intrarectal deposits of 0.1 ml solution of Methyl-N′-Nitro-N-Nitroso-Guanidine (5 mg/ml) twice a week (with a 3-day interval) for 2 weeks. Starting 2 weeks before carcinogen administration, animals were treated orally with jacalin (0.5 and 25 μg) three times a week (on alternate weekdays) for 10 weeks. We show that jacalin treatment reduced the number of preneoplastic lesions in carcinogen-exposed mice. This anticarcinogenic activity was associated with decreased colonic epithelial cell proliferation and stromal COX-2 expression and with increased intestinal production of TNF-α. Our results demonstrate that jacalin is able to modulate the early stages of colon carcinogenesis and emphasize its promising chemopreventive activity in colorectal cancer.

<p>ArtinM Grafted Phospholipid Nanoparticles for Enhancing Antibiotic Cellular Uptake Against Intracellular Infection</p>

“…This finding is supported by results obtained from the SDS-PAGE analysis given in Figures 2B and 2C . Figure 2B shows distinct two bands representing heavy and light chains at 12–15 kDa in SDS-PAGE electrophoresis gel, which is consistent with the previous report, 30 indicating the presence of jacalin in both the crude extract and jacalin dominant effluent. Further adsorption to guar gum beads, a complete artinM separation with jacalin was shown by a single band at about 16 kDa ( Figure 2C ).…”

“…The SDS-PAGE profile of artinM is recognized as a single band at 14–16 kDa, 29 while that of jacalin is as double bands at 12–15 kDa. 30 …”

“…The SDS-PAGE profile of artinM is recognized as a single band at 14-16 kDa, 29 while that of jacalin is as double bands at 12-15 kDa. 30 ArtinM activity was analyzed using haemagglutination test adopted from Sano and Ogawa, 31 in which a serial dilution of lectin in a 96-well microliter plate with the addition of erythrocyte was used. Approximately 50 µL of 2% erythrocyte suspension in PBS was placed in each well and incubated with artinM solution sample for two hours.…”

Biosynthesis and Characterization of Silver Nanoparticles (AgNPs) using the purified protein from Jack fruit seeds and its effects on HeLa, cervical cancer cell line

“…Further, the puri ed protein was used for AgNP synthesis. Jacalin, a plant lectin isolated from jackfruit seeds, is recognized for its a nity for the alpha-O-glycoside of Thomsen-Friedenreich (TF) and has been used to analyze "Olinked glycoproteins such human IgA and mucin [11][12][13]. Jackfruit seed contains more than 50% Jacalin protein, including a minor mannose-binding lectin.…”

Efeito da lectina ArtinM em células B de origem murina e células de linhagem humana de linfoma não-Hodgkin

“…Resumidamente, CD45 é relatado como o principal alvo da jacalina nas células B, promoveu a mobilização do Ca2 + intracelular e induziu a ativação da calpaína, efeito que levou à morte por apoptose (MA et al, 2009). Além disso, Jacalina é capaz de interagir com o CD45 expresso em células T CD4 + , induzindo alta produção de citocinas IL-2, IFN-γ e IL-4 (GERALDINO et al, 2017).…”