Jacareubin inhibits FcεRI-induced extracellular calcium entry and production of reactive oxygen species required for anaphylactic degranulation of mast cells

Castillo-Arellano, Jorge Iván; Guzmán-Gutiérrez, Silvia Laura; Ibarra-Sánchez, Alfredo; Hernández‐Ortega, Simón; Nieto‐Camacho, Antonio; Medina-Campos, Omar Noel; Pedraza‐Chaverrí, José; Reyes‐Chilpa, Ricardo; González-Espinosa, Claudia

doi:10.1016/j.bcp.2018.05.002

Cited by 8 publications

(10 citation statements)

References 71 publications

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“…Recent finding showed mitochondria-targeted anti-oxidant inhibited mast cell degranulation in vivo and in vitro [ 34 ]. Another study also showed jacareubin isolated from the heartwood of the tropical tree, Callophyllum brasilense , inhibited IgE-induced mast cell degranulation by its high anti-oxidant capacity [ 35 ]. Jacareubin inhibited calcium influx into mast cells accompanied with a blockage on the accumulation of the reactive oxygen species (ROS) in mast cells.…”

Section: Discussionmentioning

confidence: 99%

Intake of a fermented plant product attenuates allergic symptoms without changing systemic immune responses in a mouse model of Japanese cedar pollinosis

Fujimura

Hori

Torii³

et al. 2018

World Allergy Organization Journal

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BackgroundJapanese cedar pollinosis (JCP) is one of the most prevalent allergies in Japan. Within the past few decades, many food factors have been demonstrated to suppress symptoms of pollinosis and mast cell degranulation directly or indirectly. Herein, we conducted a study to clarify the anti-allergic potency of a fermented plant product (FPP) in JCP model mice.MethodsMice were administered FPP, 10-fold-diluted FPP, or saline every day for 40 days by oral gavage and sensitized with major Japanese cedar pollen allergens (SBP). The numbers of sneezes were counted for 5 minutes after SBP nasal challenge. We analyzed the SBP-specific immunoglobulin titers, serum concentration of mast cell protease 1, and cytokine production from splenocytes stimulated with SBP.ResultsThe numbers of sneezes by the mice administered FPP were significantly suppressed compared to those administered saline. The 10-fold-diluted FPP also suppressed the number of sneezes compared to saline, although not significantly. Serum level of mast cell protease 1 tended to be suppressed in FPP-consumed mice compared to those in saline-treated mice. The SBP-specific immunoglobulin titers and cytokine production were comparable among the groups.ConclusionsOur results suggest that FPP intake could attenuate JCP symptoms without change of systemic immune responses.

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Section: Discussionmentioning

confidence: 99%

Intake of a fermented plant product attenuates allergic symptoms without changing systemic immune responses in a mouse model of Japanese cedar pollinosis

Fujimura

Hori

Torii³

et al. 2018

World Allergy Organization Journal

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“…We previously reported the anti-inflammatory properties of Jacareubin, a xanthone molecule found in the heartwood of the American tropical tree Calophyllum brasiliense . We demonstrated that Jacareubin inhibits mast cell degranulation in vitro and passive cutaneous anaphylaxis and edema in mice, suggesting that this xanthone could be an efficient antioxidant, antiallergic, and anti-inflammatory molecule [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Jacareubin inhibits TLR4-induced lung inflammatory response caused by the RBD domain of SARS-CoV-2 Spike protein

et al. 2022

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Background COVID-19, the disease caused by SARS-CoV-2 virus infection, has been a major public health problem worldwide in the last 2 years. SARS-CoV-2-dependent activation of innate immune receptors contributes to the strong local and systemic inflammatory reaction associated with rapid disease evolution. The receptor-binding domain (RBD) of Spike (S) viral protein (S-RBD) is essential for virus infection and its interacting molecules in target cells are still under identification. On the other hand, the search for accessible natural molecules with potential therapeutic use has been intense and remains an active field of investigation. Methods C57BL6/J (control) and Toll-like receptor (TLR) 4-deficient ( Lps del ) mice were nebulized with recombinant S-RBD. Tumor Necrosis Factor-alpha (TNF-α) and Interleukin (IL)-6 production in bronchoalveolar lavages (BALs) was determined by enzyme-linked immunosorbent assay (ELISA). Lung-infiltrating cells recovered in BALs were quantified by hematoxylin–eosin (H&E) stain. In selected groups of animals, the natural compound Jacareubin or dexamethasone were intraperitoneally (ip) administered 2 hours before nebulization. Results A rapid lung production of TNF-α and IL-6 and cell infiltration was induced by S-RBD nebulization in control but not in Lps del mice. Pre-treatment with Jacareubin or dexamethasone prevented S-RBD-induced TNF-α and IL-6 secretion in BALs from control animals. Conclusions S-RBD domain promotes lung TNF-α and IL-6 production in a TLR4-dependent fashion in C57BL6/J mice. Xanthone Jacareubin possesses potential anti-COVID-19 properties that, together with the previously tested anti-inflammatory activity, safety, and tolerance, make it a valuable drug to be further investigated for the treatment of cytokine production caused by SARS-CoV-2 infection. Supplementary Information The online version contains supplementary material available at 10.1007/s43440-022-00398-5.

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“…In other words, the variation of the content of a certain component could not fully represent the degree of degranulation of MCs. The research on mast cell degranulation may contribute to elucidating the aetiology of some mast cell-related diseases [8][9][10][11] and make it possible for scientists to develop new forms of therapy for those diseases. However, the comprehensive detection of degranulation components has not been achieved.…”

Section: Introductionmentioning

confidence: 99%

“…MCs degranulation is closely related to inflammation [8], immediate allergic reactions [9], premalignant lesion and tumor growth [10]. Activation of systemic mast cells may cause headache and visceral pain [11].…”

Section: Introductionmentioning

confidence: 99%

Surface‐enhanced Raman spectroscopy analysis of mast cell degranulation induced by low‐intensity laser

Zheng

Gao

et al. 2019

IET nanobiotechnol.

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Mast cell (MC) degranulation is an important step in the healing process. In this study, silver-nanoparticles-based surface-enhanced Raman spectroscopy (SERS) was used to investigate the spectral characteristics of degranulation of MCs activated by low-intensity laser. The significant spectral changes, such as Raman peak intensities, suggested the concentration variation of some degranulated substances. The Raman intensity ratio of 799-554 cm −1 could be used as a potential internal indicator for the degranulation degree of MCs. Principal component analysis (PCA) was employed to reduce the high dimension of spectra into a few principal components (PCs) while retaining the most diagnostically significant information for sample differentiation. Using the diagnostically significant PC scores (P < 0.05), linear discriminate analysis (LDA) was applied to identify different cell degranulation groups with high sensitivity, specificity and accuracy. This exploratory work demonstrates that SERS technique combined with a PCA-LDA algorithm possesses great potential for developing a label-free, comprehensive, non-invasive and accurate method for measuring MC degranulation.

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Jacareubin inhibits FcεRI-induced extracellular calcium entry and production of reactive oxygen species required for anaphylactic degranulation of mast cells

Cited by 8 publications

References 71 publications

Intake of a fermented plant product attenuates allergic symptoms without changing systemic immune responses in a mouse model of Japanese cedar pollinosis

Intake of a fermented plant product attenuates allergic symptoms without changing systemic immune responses in a mouse model of Japanese cedar pollinosis

Jacareubin inhibits TLR4-induced lung inflammatory response caused by the RBD domain of SARS-CoV-2 Spike protein

Surface‐enhanced Raman spectroscopy analysis of mast cell degranulation induced by low‐intensity laser

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