Jagged‐1‐mediated activation of notch signalling induces adipogenesis of adipose‐derived stem cells

Ba, Kai; Yang, Xueqin; Wu, Ling; Wei, Xiaoyuan; Fu, Na; Fu, Yang; Cai, Xiaoxiao; Yuxia, Yao; Yang, Ge; Lin, Yunfeng

doi:10.1111/j.1365-2184.2012.00850.x

Cited by 40 publications

(28 citation statements)

References 40 publications

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“…Although we did not prove the form of the recombinant mouse Jagged1 protein used in this study, the increase in Hes1 and Hey1 both at protein and gene levels and the use of DAPT may explain the Jagged1 used in this study was an activator not an inhibitor. And our lab previous study also showed the recombinant Jagged1 used in this study was an activator (Ba, ) and the same results were also showed by other groups (Buchler, ; Bing, ).…”

Section: Discussionsupporting

confidence: 88%

Notch Signaling Pathway Regulates Angiogenesis via Endothelial Cell in 3D Co‐Culture Model

Zhao

Xue

Lin

et al. 2016

Journal Cellular Physiology

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This study aimed to investigate the role of Notch signaling pathway for angiogenesis in a three-dimensional (3D) collagen gel model with co-culture of adipose-derived stromal cells (ASCs) and endothelial cells (ECs). A 3D collagen gel model was established in vitro by implanting both ASCs from green fluorescent protein-labeled mouse and ECs from red fluorescent protein-labeled mouse, and the phenomena of angiogenesis with Notch signaling inducer Jagged1, inhibitor DAPT and PBS, respectively were observed by confocal laser scanning microscopy. Semi-quantitative PCR and immunofluorescent staining were conducted to detect expressions of angiogenesis-related genes and proteins. Angiogenesis in the co-culture gels was promoted by Jagged1 treatment while attenuated by DAPT treatment, compared to control group. In co-culture system of ASCs and ECs, the gene expressions of VEGFA, VEGFB, Notch1, Notch2, Hes1, Hey1, VEGFR1,and the protein expression of VEGFA, VEGFB, Notch1, Hes1, Hey1 were increased by Jagged1 treatment and decreased by DAPT treatment in ECs. And the result of VEGFR3 was the opposite. However, the same results did not appear completely in ASCs. These results revealed the VEGFA/B-Notch1/2-Hes1/Hey1- VEGFR1/3 signal axis played an important role in angiogenesis when ASCs and ECs were co-cultured in a 3D collagen gel model. J. Cell. Physiol. 232: 1548-1558, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 88%

Notch Signaling Pathway Regulates Angiogenesis via Endothelial Cell in 3D Co‐Culture Model

Zhao

Xue

Lin

et al. 2016

Journal Cellular Physiology

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“…2B,C). We then confirmed the effect of Notch inhibition by silencing the expression of JAG1, a crucial ligand for Notch signaling that induces cell differentiation (Ba et al, 2012), and which was significantly upregulated in pNCCs (Fig. 1H).…”

Section: Derivation Of Pnccs From Hescssupporting

confidence: 57%

Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells

et al. 2014

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“…3A, B). We used Oil Red O, which stains neutral triglycerides and lipids, and immunocytochemistry to look for the expression of perilipin, an essential protein for lipid storage and lipolysis [27] located exclusively on adipocyte lipid droplets [34]. Consistent with our results on PDGFRa expression, only type-1 pericytes differentiated into adipocytes in culture.…”

Section: Type-1 But Not Type-2 Pericytes Express the Adipogenic Progesupporting

confidence: 55%

Role of Pericytes in Skeletal Muscle Regeneration and Fat Accumulation

Birbrair

Zhang²,

Wang³

et al. 2013

Stem Cells and Development

258

225

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Stem cells ensure tissue regeneration, while overgrowth of adipogenic cells may compromise organ recovery and impair function. In myopathies and muscle atrophy associated with aging, fat accumulation increases dysfunction, and after chronic injury, the process of fatty degeneration, in which muscle is replaced by white adipocytes, further compromises tissue function and environment. Some studies suggest that pericytes may contribute to muscle regeneration as well as fat formation. This work reports the presence of two pericyte subpopulations in the skeletal muscle and characterizes their specific roles. Skeletal muscle from Nestin-GFP/ NG2-DsRed mice show two types of pericytes, Nestin-GFP-/NG2-DsRed + (type-1) and Nestin-GFP + /NG2-DsRed + (type-2), in close proximity to endothelial cells. We also found that both Nestin-GFP-/NG2-DsRed + and Nestin-GFP + /NG2-DsRed + cells colocalize with staining of two pericyte markers, PDGFRb and CD146, but only type-1 pericyte express the adipogenic progenitor marker PDGFRa. Type-2 pericytes participate in muscle regeneration, while type-1 contribute to fat accumulation. Transplantation studies indicate that type-1 pericytes do not form muscle in vivo, but contribute to fat deposition in the skeletal muscle, while type-2 pericytes contribute only to the new muscle formation after injury, but not to the fat accumulation. Our results suggest that type-1 and type-2 pericytes contribute to successful muscle regeneration which results from a balance of myogenic and nonmyogenic cells activation.

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Jagged‐1‐mediated activation of notch signalling induces adipogenesis of adipose‐derived stem cells

Abstract: The results led to the conclusion that activation of Notch signalling had stimulated adipogenesis of mASCs in the presence of adipogenic medium by promoting expression of PPAR-γ.

Cited by 40 publications

References 40 publications

Notch Signaling Pathway Regulates Angiogenesis via Endothelial Cell in 3D Co‐Culture Model

Notch Signaling Pathway Regulates Angiogenesis via Endothelial Cell in 3D Co‐Culture Model

Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells

Role of Pericytes in Skeletal Muscle Regeneration and Fat Accumulation

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