2013
DOI: 10.1128/jvi.01399-12
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Japanese Encephalitis Virus Infects Neuronal Cells through a Clathrin-Independent Endocytic Mechanism

Abstract: Japanese encephalitis virus (JEV) is a mosquito-borne pathogenic flavivirus responsible for acute viral encephalitis in humans. The cellular entry of JEV is poorly characterized in terms of molecular requirements and pathways. Here we present a systematic study of the internalization mechanism of JEV in fibroblasts and neuroblastoma cells. To verify the roles of distinct pathways of cell entry, we used fluorescently labeled virus particles, a combination of pharmacological inhibitors, RNA interference (RNAi), … Show more

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Cited by 122 publications
(109 citation statements)
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“…To further examine the role of GRP78 in virus entry, we performed virus binding experiments on Neuro2a cells in the presence of GRP78 antibodies. Purified JEV was labeled with the lipophilic dye DiI (Vybrant DiI cell labeling solution; Thermo Fisher Scientific) as described previously (37). Labeled virus particles retain infectivity and can be used to detect single-particle JEV entry events (37).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To further examine the role of GRP78 in virus entry, we performed virus binding experiments on Neuro2a cells in the presence of GRP78 antibodies. Purified JEV was labeled with the lipophilic dye DiI (Vybrant DiI cell labeling solution; Thermo Fisher Scientific) as described previously (37). Labeled virus particles retain infectivity and can be used to detect single-particle JEV entry events (37).…”
Section: Resultsmentioning
confidence: 99%
“…Purified JEV was labeled with the lipophilic dye DiI (Vybrant DiI cell labeling solution; Thermo Fisher Scientific) as described previously (37). Labeled virus particles retain infectivity and can be used to detect single-particle JEV entry events (37). Based on the fluorescence (A) JEV-ED3-interacting proteins from plasma membrane fractions of Neuro2a cells were separated on a 2D gel and silver stained.…”
Section: Resultsmentioning
confidence: 99%
“…86 For several viruses, involvement of Rho GTPase signaling in virus entry was reported, although the specific uptake routes are still unclear. For example, Japanese encephalitis virus (JEV), 87 poliovirus, 88 rotavirus, 89,90 and baculovirus 91 all appear to depend on RhoA activation for their entry. Porcine circovirus 2 (PCV-2) infection also appears to rely on small Rho GTPase activity for infection, as inhibition with Clostridium difficile toxin B reduced PCV-2 infection in different cell types.…”
Section: Macropinocytosismentioning
confidence: 99%
“…As mentioned above, clathrin-mediated endocytosis is reported to be the major route of cell entry for flaviviruses (Ishak et al, 1988;Ng & Lau, 1988), except for a few cases showing cell-type dependence (Kalia et al, 2013). When JEV enters host cells, the viral antigen can be detected as aggregates in CCVs or early endosomes only when Hsc70 has been knocked down in C6/36 cells.…”
Section: Discussionmentioning
confidence: 96%
“…This mechanism for infecting host cells is also implemented by various mosquito-borne flaviviruses (Acosta et al, 2008;Chu & Ng, 2004). Among them the Japanese encephalitis virus (JEV), which infects host (either mammalian or mosquito) cells via receptor-mediated endocytosis (Andoh et al, 1998;Nawa, 1998), has also been confirmed to be clathrindependent (Chai et al, 2013;Yang et al, 2013), except for certain unusual cases (Kalia et al, 2013).…”
Section: Introductionmentioning
confidence: 99%