Japanese encephalitis virus live-attenuated vaccine, Chinese strain SA14-14-2; adaptation to primary canine kidney cell cultures and preparation of a vaccine for human use

Eckels, Kenneth H.; Yu, Yongxin; Dubois, Doria R.; Marchette, Nyven J.; Trent, Dennis W.; Johnson, Anthony J.

doi:10.1016/0264-410x(88)90103-x

Cited by 86 publications

(50 citation statements)

References 8 publications

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“…Recombinant JEVmut14-14-2 and the JEV SA14-14-2 laboratory stock sequence had the least amino acid sequence divergence from the reference strain and had virulence phenotypes in suckling mice consistent with what has been reported for JEV SA14-14-2. 45 The JEV SA14-14-2 variant H differed from the JEV SA14-14-2 laboratory stock sequence by only the reversion mutation C-S66L and had a similar level of virulence. Surprisingly, Vero-adapted virus variants with more amino acid sequence divergence than variant H were significantly more attenuated in suckling mice.…”

Section: Discussionmentioning

confidence: 99%

“…22,27,45 The PDK cell-adapted virus was further adapted to Vero cells 64 and was used to generate an inactivated JEV vaccine IC-51 (IXIARO) 65 (Intercell AG), but was not developed as a live-attenuated vaccine. Here, we attempted to isolate Vero-adapted JEV SA14-14-2 variants that would be suitable live-attenuated vaccine candidates.…”

Section: Discussionmentioning

confidence: 99%

“…44 Previous attempts have been made to adapt JEV SA14-14-2 to more suitable cell substrates and have resulted in mutations in the virus that reduced vaccine immunogenicity. 22,27,45 In the current study, several genetically distinct Vero cell-adapted JEV SA14-14-2 variants were isolated and a recombinant clone bearing the polyprotein amino acid sequence of JEV SA14-14-2 was constructed and recovered in Vero cells. The in vitro and in vivo phenotypes of these viruses were compared and suggest that Vero cells are a suitable substrate for the development of a safe and immunogenic, second-generation, live-attenuated JEV SA14-14-2 vaccine.…”

Section: Introductionmentioning

confidence: 99%

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Genetic and Phenotypic Properties of Vero Cell-Adapted Japanese Encephalitis Virus SA14-14-2 Vaccine Strain Variants and a Recombinant Clone, Which Demonstrates Attenuation and Immunogenicity in Mice

Gromowski

Firestone

Bustos-Arriaga

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The live-attenuated Japanese encephalitis virus (JEV) SA14-14-2 vaccine, produced in primary hamster kidney cells, is safe and effective. Past attempts to adapt this virus to replicate in cells that are more favorable for vaccine production resulted in mutations that significantly reduced immunogenicity. In this study, 10 genetically distinct Vero cell-adapted JEV SA14-14-2 variants were isolated and a recombinant wild-type JEV clone, modified to contain the JEV SA14-14-2 polyprotein amino acid sequence, was recovered in Vero cells. A single capsid protein mutation (S66L) was important for Vero cell-adaptation. Mutations were also identified that modulated virus sensitivity to type I interferonstimulation in Vero cells. A subset of JEV SA14-14-2 variants and the recombinant clone were evaluated in vivo and exhibited levels of attenuation that varied significantly in suckling mice, but were avirulent and highly immunogenic in weanling mice and are promising candidates for the development of a second-generation, recombinant vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic and Phenotypic Properties of Vero Cell-Adapted Japanese Encephalitis Virus SA14-14-2 Vaccine Strain Variants and a Recombinant Clone, Which Demonstrates Attenuation and Immunogenicity in Mice

Gromowski

Firestone

Bustos-Arriaga

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…The attenuated SA14-14-2 vaccine strain, adapted to primary canine kidney cells, 39 was further passaged in Vero cells. Vero cells used for production were a derivative of a certified cell line that has been used to produce more than 1 billion doses of licensed polio and rabies vaccines.…”

Section: Productionmentioning

confidence: 99%

Product review on the JE vaccine IXIARO

Firbas

Jilma

2015

Human Vaccines & Immunotherapeutics

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“…These include the dengue-2 and 4 vaccines, as well as the attenuated Japanese encephalitis virus vaccine. Research describing the current state of development and testing of these vaccines has been described (1,2,5,29,30,(48)(49)(50)(51)54,73,87,117,118).…”

Section: Vaccine Developmentmentioning

confidence: 99%

Insect-transmitted vertebrate viruses: Flaviviridae

Ludwig

Iacono-Connors

1993

In Vitro Cell Dev Biol - Animal

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SUMMARYThe Flaviviridae include almost 70 viruses, nearly half of which have been associated with human disease. These viruses are among the most important arthropod-borne viruses worldwide and include dengue, yellow fever, and Japanese encephalitis viruses. Morbidity and mortality caused by these viruses vary, but collectively they account for millions of encephalitis, hemorrhagic fever, arthralgia, rash, and fever cases per year. Most of the members of this family are transmitted between vertebrate hosts by arthropod vectors, most commonly mosquitoes or ticks. Transmission cycles can be simple or complex depending on the hosts, vectors, the virus, and the environmental factors affecting both hosts and viruses. Replication of virus in invertebrate hosts does not seem to result in any significant pathology, which suggests a close evolutionary relationship between virus and vector. Another example of this relationship is the ability of these viruses to grow in invertebrate ceil culture, where replication usually results in a steady state, persistent infection, often without cytopathic effect. Yields of virus from insect cell culture vary but are generally similar to yields in vertebrate cells. Replication kinetics are comparable between insect and vertebrate ceil lines, despite differences in incubation temperature. Both vertebrate and insect cell culture systems continue to play a significant role in flavivirus isolation and the diagnosis of disease caused by these agents. Additionally, these culture systems permit the study of flavivirus attachment, penetration, replication, and release from cells and have been instrumental in the production and characterization of live-attenuated vaccines. Both vertebrate and insect cell culture systems will continue to play a significant role in basic and applied flavivirus research in the future.

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Japanese encephalitis virus live-attenuated vaccine, Chinese strain SA14-14-2; adaptation to primary canine kidney cell cultures and preparation of a vaccine for human use

Cited by 86 publications

References 8 publications

Genetic and Phenotypic Properties of Vero Cell-Adapted Japanese Encephalitis Virus SA14-14-2 Vaccine Strain Variants and a Recombinant Clone, Which Demonstrates Attenuation and Immunogenicity in Mice

Genetic and Phenotypic Properties of Vero Cell-Adapted Japanese Encephalitis Virus SA14-14-2 Vaccine Strain Variants and a Recombinant Clone, Which Demonstrates Attenuation and Immunogenicity in Mice

Product review on the JE vaccine IXIARO

Insect-transmitted vertebrate viruses: Flaviviridae

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