Japanese Sake‐Brewing Rice Cultivars Show High Levels of Globulin‐like Protein and a Chloroplast Stromal HSP70

Kamara, Joseph Sherman; Hoshino, Miki; Satoh, Yuki; Nayar, N. K.; Takaoka, Motoko; Sasanuma, Tsuneo; Abe, Toshinori

doi:10.2135/cropsci2008.12.0719

Cited by 3 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein abundance values in the embryo were lower than in cotyledon and a general trend was not observed. Some of them, like alanine aminotransferase, have been reported to play a role in seed dormancy [47].…”

Section: Discussionmentioning

confidence: 99%

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Sghaier-Hammami

Hammami

Baazaoui

et al. 2020

IJMS

View full text Add to dashboard Cite

Unlike orthodox species, seed recalcitrance is poorly understood, especially at the molecular level. In this regard, seed maturation and germination were studied in the non-orthodox Quercus ilex by using a proteomics strategy based on two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization/time of flight (2-DE-MALDI-TOF).Cotyledons and embryo/radicle were sampled at different developmental stages, including early (M1–M3), middle (M4–M7), and late (M8–M9) seed maturation, and early (G1–G3) and late (G4–G5) germination. Samples corresponding to non-germinating, inviable, seeds were also included. Protein extracts were subjected to 2-dimensional gel electrophoresis (2-DE) and changes in the protein profiles were analyzed. Identified variable proteins were grouped according to their function, being the energy, carbohydrate, lipid, and amino acid metabolisms, together with protein fate, redox homeostasis, and response to stress are the most represented groups. Beyond the visual aspect, morphometry, weight, and water content, each stage had a specific protein signature. Clear tendencies for the different protein groups throughout the maturation and germination stages were observed for, respectively, cotyledon and the embryo axis. Proteins related to metabolism, translation, legumins, proteases, proteasome, and those stress related were less abundant in non-germinating seeds, it related to the loss of viability. Cotyledons were enriched with reserve proteins and protein-degrading enzymes, while the embryo axis was enriched with proteins of cell defense and rescue, including heat-shock proteins (HSPs) and antioxidants. The peaks of enzyme proteins occurred at the middle stages (M6–M7) in cotyledons and at late ones (M8–M9) in the embryo axis. Unlike orthodox seeds, proteins associated with glycolysis, tricarboxylic acid cycle, carbohydrate, amino acid and lipid metabolism are present at high levels in the mature seed and were maintained throughout the germination stages. The lack of desiccation tolerance in Q. ilex seeds may be associated with the repression of some genes, late embryogenesis abundant proteins being one of the candidates.

show abstract

Section: Discussionmentioning

confidence: 99%

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Sghaier-Hammami

Hammami

Baazaoui

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Two distinct members of the Hsp70 family of stress-related protein were localized in the maize amyloplast and form transient complexes with starch synthase 1 (SSI) and other stromal enzymes [37]. In Japanese sake-brewing rice rich in starch content, the HSP70 protein was highly abundant in amyloplast compared to cytosol and its concentration was elevated during the later stages of grain development [50]. Our candidate HSP90 gene showed strong protein-protein interaction with numerous molecular chaperones including several HSP70 proteins.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum

Sapkota

Boatwright

Jordan

et al. 2020

Genes

View full text Add to dashboard Cite

Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition. Unraveling genetic control for starch metabolism is important for breeding grains with high starch content. In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content. Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM. All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm. The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing. The first interactors of HSP90 also showed high transcript abundance in reproductive tissues. The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.

show abstract

“…It is not produced by a conventional beer brewing process, but there are several similarities between beer and sake (Hardwick, 1995). Sake is made from steamed rice by a double fermentation process involving koji (A. oryzae) and yeast (S. cerevisiae) (Kamara et al, 2009). The fi rst step in sake brewing is polishing of rice.…”

Section: Sakementioning

confidence: 99%

Rice

Arendt¹,

Zannini²

2013

Cereal Grains for the Food and Beverage Industries

View full text Add to dashboard Cite

Rice (Oriza sativa L.) is the staple food for nearly two-thirds of the world's population. In 2010, China, India, Indonesia, Bangladesh, Vietnam and Myanmar alone provided more than 75 % of the world's total rice production. Rice has the lowest protein content of all the cereals; however, rice protein is highly nutritious and has one of the highest lysine contents among the cereals. Rice is rich in starch, low in fi bre and has no detectable levels of vitamins A, C or D. Rice is consumed in the form of noodles, puffed rice, fermented sweet rice and snack foods produced by extrusion cooking. It is used for making bakery products, sauces, infant foods, breakfast cereals, alcoholic beverages and vinegar. Continuing population growth and consequent rice consumption increases will mean that the global demand for rice will expand signifi cantly in the near future. An integrated approach that involves more effi cient and environmentally sustainable agricultural practices, pest management and the development of new rice varieties adapted to country-specifi c farming conditions suitable for a changing climate should be vigorously pursued.

show abstract

Japanese Sake‐Brewing Rice Cultivars Show High Levels of Globulin‐like Protein and a Chloroplast Stromal HSP70

Cited by 3 publications

References 30 publications

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum

Rice

Contact Info

Product

Resources

About