Jiedu Sangen Decoction Inhibits Migration and Invasion of Colon Cancer SW480 Cells via Suppressing Epithelial Mesenchymal Transition

Zhang, Kai; Peng, Tao; Yan, Qingying; Sun, Leitao; Miao, Haojun; Li, Yuan; Hong, Yuxin; Zhou, Mengmeng; Guo, Kaibo; Zhang, Leyin; Fang, Jing; Shen, Minhe; Ruan, Shanming

doi:10.1155/2018/1495768

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…Recent studies have revealed that the Hippo pathway may be involved in the EMT process [19, 20]. In our previous preliminary study, it was observed that JSD could inhibit the invasion and metastasis of the CRC cells [21]. Hence, the aim of the present study was to further investigate whether the Hippo pathway could be the key mechanism involved in the process by which JSD regulates EMT in CRC.…”

Section: Introductionmentioning

confidence: 93%

Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway

Zhou

Wasan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Colorectal cancer (CRC) is one of the most common malignant tumors affecting the digestive tract. Moreover, the invasion and metastasis of CRC are the main reason therapy is usually inefficient. Decreased intercellular adhesion and enhanced cell motility induced by epithelial-mesenchymal transition (EMT) provide the basic conditions for the invasion and metastasis of the epithelial tumor cells of CRC. The Jiedu Sangen Decoction (JSD) is a prescription that has been used for more than 50 years in the treatment of CRC in the Zhejiang Hospital of Traditional Chinese Medicine. The aim of this study was to investigate the mechanism of JSD-triggered inhibition of invasion and metastasis in colon cancer. In vitro, the EMT model of the SW480 cells was induced by using epithelial growth factor (50 ng/mL). In vivo, the murine model of liver metastasis was constructed by inoculating mice with the SW480 cells. The effects of JSD on cell migration, invasion, and proliferation were determined using the transwell assay and CCK-8 assay. Moreover, the proteins related to the EMT process and the Hippo signaling pathway in the cancerous tissues and cell lines were determined by western blotting and immunostaining. JSD could significantly inhibit the proliferation, migration, and invasion of CRC cells and reverse their EMT status (all, P < 0.05). Moreover, after intervention with JSD, the levels of E-Cadherin (E-cad) increased, whereas the expression levels of N-Cadherin (N-cad), Yes-associated protein (YAP), and the transcriptional coactivator with the PDZ-binding motif (TAZ) decreased in both the SW480 cells and the tumor tissues. In summary, JSD reversed EMT and inhibited the invasion and metastasis of CRC cells through the Hippo signaling pathway.

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Section: Introductionmentioning

confidence: 93%

Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway

Zhou

Wasan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Danshensu inhibits platelet-induced EMT transformation and chemoresistance in CRC cells ( 140 ). Jiedu Sangen Decoction inhibits the AKT/GSK-3β signaling pathway, thereby reversing EMT ( 141 , 142 ). Resveratrol strongly inhibited the formation of EMT and CSC induced by TNF-β, downregulated cancer-promoting factors such as vimentin and slug, upregulated E-cadherin and claudin-2, increased the tight junction and cell adhesion, inhibited NF-κB activation, and increased 5-FU sensitivity in 5-FU-resistant CRC ( 143 , 144 ).…”

Section: Tcm Reverses the Chemoresistance Of Crc And Inhibits Tumor G...mentioning

confidence: 99%

Research progress of traditional Chinese medicine as sensitizer in reversing chemoresistance of colorectal cancer

Lin

Yang

et al. 2023

Front. Oncol.

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In recent years, the incidences and mortalities from colorectal cancer (CRC) have been increasing; therefore, there is an urgent need to discover newer drugs that enhance drug sensitivity and reverse drug tolerance in CRC treatment. With this view, the current study focuses on understanding the mechanism of CRC chemoresistance to the drug as well as exploring the potential of different traditional Chinese medicine (TCM) in restoring the sensitivity of CRC to chemotherapeutic drugs. Moreover, the mechanism involved in restoring sensitivity, such as by acting on the target of traditional chemical drugs, assisting drug activation, increasing intracellular accumulation of anticancer drugs, improving tumor microenvironment, relieving immunosuppression, and erasing reversible modification like methylation, have been thoroughly discussed. Furthermore, the effect of TCM along with anticancer drugs in reducing toxicity, increasing efficiency, mediating new ways of cell death, and effectively blocking the drug resistance mechanism has been studied. We aimed to explore the potential of TCM as a sensitizer of anti-CRC drugs for the development of a new natural, less-toxic, and highly effective sensitizer to CRC chemoresistance.

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“…11,12 Numerous studies have revealed that PD-L1, as expressed by tumor cells, can promote EMT [13][14][15][16][17] ; one study reported that PD-L1 may induce EMT via the PI3K/AKT pathway. 17 Jiedu Sangen Decoction (JSD) is a Chinese herbal formulation exploited by us as a supplementary treatment of CRC, 18,19 comprising 3 Chinese traditional herbs: Teng Li Gen (Radix Actinidiae chinensis), Shui Yang Mei Gen (Root of Thinleaf Adina) and Hu Zhang Gen (Polygoni Cuspidati Radix). The known constituents of this concoction, including ursolic Acid, 12-en-28-oic acids of oleananetype (from Radix Actinidiae chinensis) and emodin (from Polygoni Cuspidati Radix), have been proved to have antitumor potential via the PI3K/AKT signaling pathway.…”

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confidence: 99%

Inhibition to Epithelial-Mesenchymal Transition and Metastatic Potential In Colorectal Cancer Cell By Combination of Traditional Chinese Medicine Formulation Jiedu Sangen Decoction and PD-L1 Inhibitor

et al. 2020

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Background: Jiedu Sangen Decoction (JSD), a traditional Chinese medicine formula, has been widely applied in the treatment of gastrointestinal cancer, especially in colorectal cancer. Our study mainly aimed to assess the combined efficacy of Jiedu Sangen aqueous extract (JSAE) and a PD-L1 inhibitor (PI) in colon cancer cells migration and invasion, along with epithelial-mesenchymal transition, and then provide deep insights into the potential mechanism. Methods: We explored the inhibitory effects on invasion and metastasis and the reverse effect on EMT process in CT-26 colon cancer cell via Transwell migration assay, Matrigel invasion assay and confocal laser scanning microscopy. Furthermore, regulation in expression of EMT-related proteins and molecular biomarkers and underlying signal pathway proteins were detected through Western blotting and IHC. Results: The combination of JSD and PD-L1 inhibitor could inhibit migration, invasive ability and EMT of CT-26 cells in a concentration-dependent manner. Meanwhile, JSD combined with PD-L1 inhibitor could also remarkably reverse EMT and metastasis in vivo. In addition, the protein expression of N-cadherin, Slug, Snail, Vimentin was down-regulated along with E-cadherin s up-regulation with the combination of JSD and PD-L1 inhibitor, while that of PI3K/AKT was notably down-regulated. Conclusions: These findings indicated that JSAE and a PD-L1 inhibitor could drastically inhibit the migration and invasion of colorectal cancer by reversing EMT through the PI3K/AKT signaling pathway.

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Jiedu Sangen Decoction Inhibits Migration and Invasion of Colon Cancer SW480 Cells via Suppressing Epithelial Mesenchymal Transition

Cited by 7 publications

References 44 publications

Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway

Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway

Research progress of traditional Chinese medicine as sensitizer in reversing chemoresistance of colorectal cancer

Inhibition to Epithelial-Mesenchymal Transition and Metastatic Potential In Colorectal Cancer Cell By Combination of Traditional Chinese Medicine Formulation Jiedu Sangen Decoction and PD-L1 Inhibitor

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