JK-2 loaded electrospun membrane for promoting bone regeneration

Al-Bishari, Abdullrahman M.; Yie, Kendrick Hii Ru; Al-Baadani, Mohammed A.; Al-Shaaobi, Bilal A.; Zhou, Zixin; Fang, Kai; Sun, Anba; Shen, Yiding; Cai, Lei; Yao, Lili; Ding, Xi; Shen, Xinkun; Liu, Jinsong

doi:10.1016/j.msec.2021.112471

Cited by 10 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, porous electrospinning scaffolds can be loaded with proteins and drugs using some specific technologies [ 23 ]. In the past, researchers have developed electrospinning bone scaffolds for skull defects and alveolar bone defects regeneration [ 24 , 25 ]. Electrospinning periosteum showed the capability of accelerating bone regeneration and promoting angiogenesis [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

The polycaprolactone/silk fibroin/carbonate hydroxyapatite electrospun scaffold promotes bone reconstruction by regulating the polarization of macrophages

Jia

Zhou

Ning

et al. 2022

Regenerative Biomaterials

View full text Add to dashboard Cite

Macrophages are known to modulate the osteogenic environment of bone regeneration elicited by biological bone grafts. Alteration in certain chemical components tends to affect macrophages polarization. Comparatively to hydroxyapatite (HAp), carbonate hydroxyapatite (CHA) consists of 7.4 (wt%) carbonate ions and more closely resembles the mineral content of bone. It remains unknown whether CHA scaffolds or HA scaffolds have better osteogenic properties. In this study, we fabricated PCL/SF scaffold, PCL/SF/HAp scaffold and PCL/SF/CHA scaffold using the electrospinning technique. Despite comparable mechanical properties, the PCL/SF/CHA scaffold exhibited better osteogenic properties than the PCL/SF/HAp scaffold. Although no significant differences were observed between the two scaffolds for promoting osteoblast differentiation in vitro, the PCL/SF/CHA group appeared to be more effective at promoting bone regeneration in cranial defects in vivo. The PCL/SF/CHA scaffold was found to promote macrophage polarization toward M2 via activating the JAK/STAT5 pathway which caused a pro-osteogenic microenvironment to facilitate osteoblast differentiation. The results of this study indicated a higher potential of CHA to substitute HAp in the production of bone scaffolds for better bone regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

The polycaprolactone/silk fibroin/carbonate hydroxyapatite electrospun scaffold promotes bone reconstruction by regulating the polarization of macrophages

Jia

Zhou

Ning

et al. 2022

Regenerative Biomaterials

View full text Add to dashboard Cite

show abstract

“…Animal experiments further confirm that JK-2-loaded PCL/Gel scaffolds promote bone tissue formation, improve bone tissue microstructure and mechanical properties, and increase mineral density. 103 In a separate study, GelMA was combined with tricalcium phosphate (TCP) to enhance the bioactivity of PCL. TCP is a cell-mediated, osteoinductive, and resorbable synthetic bone graft substitute that was incorporated to improve the biocompatibility and bioactivity of the scaffolds.…”

Section: Materials For Nanofiber Tissue Engineering Scaffoldsmentioning

confidence: 99%

“…Cell culture investigations conducted in a laboratory setting show that the inclusion of JK-2 donor H2S substantially enhances ALP activity, mineralization, and genes associated with the formation of bone tissue, hence encouraging the development of osteoblasts. Animal experiments further confirm that JK-2-loaded PCL/Gel scaffolds promote bone tissue formation, improve bone tissue microstructure and mechanical properties, and increase mineral density …”

Section: Materials For Nanofiber Tissue Engineering Scaffoldsmentioning

confidence: 99%

Application and Development of Electrospun Nanofiber Scaffolds for Bone Tissue Engineering

Huang,

Zeng,

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Nanofiber scaffolds have gained significant attention in the field of bone tissue engineering. Electrospinning, a straightforward and efficient technique for producing nanofibers, has been extensively researched. When used in bone tissue engineering scaffolds, electrospun nanofibers with suitable surface properties promote new bone tissue growth and enhance cell adhesion. Recent advancements in electrospinning technology have provided innovative approaches for scaffold fabrication in bone tissue engineering. This review comprehensively examines the utilization of electrospun nanofibers in bone tissue engineering scaffolds and evaluates the relevant literature. The review begins by presenting the fundamental principles and methodologies of electrospinning. It then discusses various materials used in the production of electrospun nanofiber scaffolds for bone tissue engineering, including natural and synthetic polymers, as well as certain inorganic materials. The challenges associated with these materials are also described. The review focuses on novel electrospinning techniques for scaffold construction in bone tissue engineering, such as multilayer nanofibers, multifluid electrospinning, and the integration of electrospinning with other methods. Recent advancements in electrospinning technology have enabled the fabrication of precisely aligned nanofiber scaffolds with nanoscale architectures. These innovative methods also facilitate the fabrication of biomimetic structures, wherein bioactive substances can be incorporated and released in a controlled manner for drug delivery purposes. Moreover, they address issues encountered with traditional electrospun nanofibers, such as mechanical characteristics and biocompatibility. Consequently, the development and implementation of novel electrospinning technologies have revolutionized scaffold fabrication for bone tissue engineering.

show abstract

“…Several studies have shown that the slow degradation rate makes it useful for a time-controlled release of medications over an extended period of time ( Castro et al, 2018 ; Liu et al, 2020 ; Vonbrunn et al, 2020 ; Al-Baadani et al, 2021 ). Therefore, it has been used mainly as a long-term implant material for drug release (especially hydrophilic drugs) and supports bone formation ( Al-Bishari et al, 2021 ). It has come to our understanding that vitamin D (Vit D) plays a major role in maintaining bone mineral density in the human body.…”

Section: Introductionmentioning

confidence: 99%

Vitamin D and curcumin-loaded PCL nanofibrous for engineering osteogenesis and immunomodulatory scaffold

Al-Bishari

Al-Shaaobi²,

Al-Bishari³

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

The accelerating bone healing process is still a major challenge in clinical orthopedics, especially in critical-sized bone defects. Recently, Nanofiber membranes are showing increasing attention in the biomedical field due to their good biocompatibility, mechanical stability, and the ability to work as a drug carrier to achieve localized and sustained drug delivery. Herein, a multifunction nanofiber membrane loaded with vitamin D (Vit D) and curcumin (Cur) was successfully fabricated using electrospinning technology. In addition, we innovatively modified Vit D with PEG to improve the hydrophilicity of PCL nanofibers. The vitro results of CCK-8, alkaline phosphatase (ALP) and mineralization demonstrated that the PCL/Vit D-Cur membrane had great potential for enhancing the proliferation/differentiation of osteoblasts. Moreover, the synergistic effect of Vit D-Cur loaded PCL nanofiber membrane showed a superior ability to improve the anti-inflammatory activity through M2 polarization. Furthermore, in vivo results confirmed that the defect treated with PCL/Vit D-Cur nanofiber membrane was filled with the newly formed bone after 1 month. These results indicate that the Vit D/Cur loaded membrane can be applied for potential bone regeneration therapy.

show abstract

JK-2 loaded electrospun membrane for promoting bone regeneration

Cited by 10 publications

References 45 publications

The polycaprolactone/silk fibroin/carbonate hydroxyapatite electrospun scaffold promotes bone reconstruction by regulating the polarization of macrophages

The polycaprolactone/silk fibroin/carbonate hydroxyapatite electrospun scaffold promotes bone reconstruction by regulating the polarization of macrophages

Application and Development of Electrospun Nanofiber Scaffolds for Bone Tissue Engineering

Vitamin D and curcumin-loaded PCL nanofibrous for engineering osteogenesis and immunomodulatory scaffold

Contact Info

Product

Resources

About