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Delarue, Catherine; Contesse, Vincent; Lenglet, Sébastien; Sicard, Flavie; Perraudin, Véronique; Lefebvre, Henri; Kodjo, Magloire K.; Leboulenger, F.; Yon, Laurent; Gallo‐Payet, Nicole; Vaudry, Hubert

doi:10.1023/a:1011512415497

Cited by 38 publications

(7 citation statements)

References 183 publications

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“…In summary, the present study indicates that (i) ghrelin and GHS‐R are both expressed in the rat adrenal cortex; (ii) ghrelin binding sites are abundant in the outer cortex; (iii) ghrelin does not affect the secretory activity of rat adrenocortical cells; (iv) ghrelin stimulates proliferation of cultured ZG cells, without significantly affecting apoptotic deletion rate; and (v) the ZG proliferogenic action of ghrelin involves the TK‐dependent activation of the p42/p44 MAPK cascade. In conclusion, our results allow us to include ghrelin in that group of peptides [44,45] involved in the autocrine–paracrine regulation of adrenal growth.…”

Section: Resultsmentioning

confidence: 78%

Ghrelin and growth hormone secretagogue receptor are expressed in the rat adrenal cortex: evidence that ghrelin stimulates the growth, but not the secretory activity of adrenal cells

Andreis

Malendowicz²,

Trejter³

et al. 2003

FEBS Letters

100

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Ghrelin is an endogenous ligand of the growth hormone secretagogue receptor (GHS-R), which has been originally isolated from rat stomach. Evidence has been previously provided that adrenal gland possesses abundant ghrelin-displaceable GHS-Rs, but nothing is known about the possible role of ghrelin in the regulation of adrenocortical function. Reverse transcription-polymerase chain reaction demonstrated the expression of ghrelin and GHS-R in the rat adrenal cortex, and high adrenal concentrations of immunoreactive ghrelin were detected by radioimmune assay (RIA). Autoradiography localized abundant [ 125 I]ghrelin binding sites in the adrenal zona glomerulosa (ZG) and outer zona fasciculata (ZF). Ghrelin (from 10 310 to 10 38 M) did not a¡ect either basal steroid hormone (pregnenolone, progesterone, 11-deoxycorticosterone, corticosterone, 18-hydroxycorticosterone and aldosterone) secretion from dispersed ZG and zona fasciculata/reticularis (ZF/R) cells (as evaluated by quantitative high pressure liquid chromatography), or basal and agonist-stimulated aldosterone and corticosterone production from cultured ZG and ZF/R cells, respectively (as measured by RIA). Ghrelin (10 38 and 10 36 M) raised basal, but not agonist-stimulated, proliferation rate of cultured ZG cells (percent of cells able to incorporate 5-bromo-2P P-deoxyuridine), without a¡ecting apoptotic deletion rate (percent of cells able to incorporate biotinylated nucleosides into apoptotic DNA fragments). The tyrosine kinase (TK) inhibitor tyrphostin-23 and the p42/p44 mitogen-activated protein kinase (MAPK) inhibitor PD-98059 abolished the proliferogenic e¡ect of 10 38 M ghrelin, while the protein kinase A and C inhibitors H-89 and calphostin-C were ine¡ective. Ghrelin (10 38 M) stimulated TK and MAPK activity of dispersed ZG cells, and the e¡ect was abolished by preincubation with tyrphostin-23 and PD-98059, respectively. Tyrphostin-23 annulled ghrelin-induced activation of MAPK activity. Taken together, the present ¢ndings indicate that (i) ghrelin and GHS-R are both expressed in the rat adrenal cortex, ghrelin binding sites being very abundant in the ZG; (ii) ghrelin does not a¡ect the secretory activity of rat adrenocortical cells, but signi¢cantly enhances the proliferation rate of cultured ZG cells, without a¡ecting apoptotic deletion rate; and (iii) the ZG proliferogenic action of ghrelin involves the TK-dependent activation of the p42/p44 MAPK cascade. ß

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Section: Resultsmentioning

confidence: 78%

Ghrelin and growth hormone secretagogue receptor are expressed in the rat adrenal cortex: evidence that ghrelin stimulates the growth, but not the secretory activity of adrenal cells

Andreis

Malendowicz²,

Trejter³

et al. 2003

FEBS Letters

100

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“…This lack of personal protection use away from home is common in Southeast Asia [30,34], and this phenomenon has been targeted as part of the RBM initiative, with NGOs investigating the potential for Social Marketing of hammock nets and deet (di-ethyl toulamide) based repellents for migratory populations [35]. Additionally, the mandatory use of personal protection in the field by plantation workers or those working for commercial logging companies in Southeast Asia could be negotiated as has occurred in Bolivia [41]. …”

Section: Discussionmentioning

confidence: 99%

Border malaria in China: knowledge and use of personal protection by minority populations and implications for malaria control: a questionnaire-based survey

Moore

Xia²,

Hill

et al. 2008

BMC Public Health

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The use of personal protection must be increased, particularly among outdoor workers that have higher malaria risk. However, personal protection is widely used and widely accepted to prevent nuisance biting mosquitoes, with the major barrier to use being affordability. Therefore, social marketing campaigns aimed at women and those that work outdoors that provide highly subsidised products, especially insecticide impregnation kits for bednets and hammock nets are most likely to succeed in lowering malaria morbidity among non Han-Chinese groups in rural China.

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“…We observed poorly differentiated cells in the subcapsular zone, which can be explained by high proliferative activity of the cambial reserve. According to some authors, ACC can proliferate in any adrenal zone under the effect of endogenous substances released by chromaffin cells, immune cells, and endotheliocytes [10,14].…”

Section: Resultsmentioning

confidence: 99%

Morphogenesis of Adaptation and Compensatory Reactions in Mouse Adrenals during Restitution after Thermal Exposure

et al. 2005

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Single hyperthermia session causes an appreciable decrease in adrenocorticocyte count in the adrenal cortex in mice on day 3 of postthermal restitution. Morphological changes in the adrenals under conditions of developing stress reaction manifested in increased structural and functional heterogeneity of adrenocorticocytes in all cortical layers, exhaustion of lipid incorporations in the zona fasciculata cells, pronounced shrinkage of zona reticularis, developing against the background of hemodynamic disorders. Recovery of the total count of adrenocorticocyte population was associated with the appearance of small accumulations of immature cells in the subcapsular area of zona glomerulosa. Despite the absence of pronounced changes in the architectonics of all adrenocortical zones during postthermal restitution, recovery of zona reticularis was incomplete, which manifested in its shrinkage and persistence of degenerative changes in adrenocorticocytes.

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Cited by 38 publications

References 183 publications

Ghrelin and growth hormone secretagogue receptor are expressed in the rat adrenal cortex: evidence that ghrelin stimulates the growth, but not the secretory activity of adrenal cells

Ghrelin and growth hormone secretagogue receptor are expressed in the rat adrenal cortex: evidence that ghrelin stimulates the growth, but not the secretory activity of adrenal cells

Border malaria in China: knowledge and use of personal protection by minority populations and implications for malaria control: a questionnaire-based survey

Morphogenesis of Adaptation and Compensatory Reactions in Mouse Adrenals during Restitution after Thermal Exposure

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