This study is focused on examining how organizational culture can affect the relationship between variables, namely the variables of job satisfaction and work motivation on employee performance with the object of employees in the National SAR Agency of Semarang City. The population in this study was 124, and all of them were used as respondents. The statistical analysis method used is regression analysis, using the SPPS version 19 software. Based on the results of statistical tests, to test instruments for both validity and reliability, all indicators used to measure these variables are valid and reliable. Furthermore, based on the significance test, it is proven that work motivation and job satisfaction have a positive and significant effect on employee performance. Meanwhile, organizational culture moderates the relationship between work motivation and performance; and job satisfaction with performance, which has a good effect, meaning that a good organizational culture will have a stronger impact on the influence of these variables. In the future, it is expected to always create a conducive organizational culture so that organizational nuances can be maintained and guaranteed so that work motivation and job satisfaction will always be the basis for improving employee performance.