This research was conducted to analyze the influence of organizational culture and work environment on lecturer performance with intrinsic motivation as an intervening variable. This research was conducted with sample of 80 lecturer respondents at the University of Faletehan was taken using convenience sampling / accidental sampling. The data analysis method uses the SEM method, it was smart PLS. Based on the results of the reasearch, it was concluded that organizational culture had a positive and significant direct effect on lecturer performance, the work environment had a positive and insignificant direct effect on lecturer performance, organizational culture had a positive and significant impact. Against intrinsic motivation, work environment has a positive and significant impact toward intrinsic motivation, intrinsic motivation positive and significant influence toward the performance of lecturers. The indirect relationship is organizational culture has an indirect effect on lecturer performance through intrinsic motivation, and the work environment had an indirect effect on lecturer performance through intrinsic motivation.