Journal of Plant Protection Research

Trzmiel, Katarzyna

doi:10.24425/jppr.2020.133959

Cited by 4 publications

References 13 publications

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Two major quantitative trait loci control wheat dwarf virus resistance in four related winter wheat populations

Buerstmayr

2023

Theor Appl Genet

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Key message Qwdv.ifa-6A on chromosomes 6AL and Qwdv.ifa-1B on chromosome 1B are highly effective against wheat dwarf virus and act additively when combined. Abstract Wheat dwarf virus (WDV) is among the most damaging viral pathogens. Its prevalence has increased substantially in recent years, and it is predicted to increase even further due to global warming. There are limited options to control the virus. Growing resistant cultivars would safeguard crops, but most current wheat cultivars are highly susceptible. Thus, the aim of this study was to dissect the genetic architecture of WDV resistance in resistant germplasm and to identify quantitative trait loci (QTL) to support resistance breeding. QTL mapping was conducted using four related populations comprising 168, 105, 99 and 130 recombinant inbred lines. Populations were evaluated under field conditions for three years. Natural infestation was provoked by early autumn sowing. WDV symptom severity was visually assessed at two time points in spring. QTL analysis revealed two highly significant QTL with the major QTL Qwdv.ifa-6A mapping to the long arm of chromosome 6A between markers Tdurum_contig75700_411 (601,412,152 bp) and AX-95197581 (605,868,853 bp). Qwdv.ifa-6A descends from the Dutch experimental line SVP-72017 and was of high effect in all populations, explaining up to 73.9% of the phenotypic variance. The second QTL, Qwdv.ifa-1B, mapped to chromosome 1B and is putatively associated with the 1RS.1BL translocation, which was contributed by the CIMMYT line CM-82036. Qwdv.ifa-1B explained up to 15.8% of the phenotypic variance. Qwdv.ifa-6A and Qwdv.ifa-1B are among the first identified highly effective resistance QTL and represent valuable resources for improving WDV resistance in wheat.

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Two major quantitative trait loci control wheat dwarf virus resistance in four related winter wheat populations

Buerstmayr

2023

Theor Appl Genet

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Development of loop-mediated isothermal amplification assay for rapid detection of genetically different wheat dwarf virus isolates

Trzmiel

Hasiów‐Jaroszewska

2020

Mol Biol Rep

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Wheat dwarf virus (WDV) is considered as one of the most common viruses on cereal crops. Recently, severe outbreaks of WDV have been observed especially on winter wheat in southwestern part of Poland. Moreover, the presence of genetically different WDV-barley-specific and WDV-wheat-specific forms (WDV-B and WDV-W, respectively) was confirmed. In this study, a loop-mediated isothermal amplification assay (LAMP) was developed for the first time for efficient and rapid detection of WDV-B and WDV-W in infected plants. The reaction was performed using a set of three primer pairs: WDVF3/WDVB3, WDVFIB/WDVBIP and WDVLoopF/WDVLoopB specific for coat protein coding sequence. The amplified products were analyzed by direct staining of DNA, gel electrophoresis and real-time monitoring of the amplification curves. The sensitivity of optimized reaction was tenfold higher in comparison with conventional PCR. LAMP assay developed here is a useful and practical method for the rapid detection of different WDV isolates and can be implemented by phytosanitary services.

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Development of a droplet digital PCR assay to detect and quantify BYDV-MAV and BYDV-PAS in their barley host and aphid vectors

Ballandras,

McNamara,

Carolan

et al. 2024

Irish Journal of Agricultural and Food Research

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Barley yellow dwarf viruses (BYDVs) belong to a complex of several species, all vectored by aphids. Due to the abundance of Sitobion avenae and Rhopalosiphum padi, BYDV-MAV and BYDV-PAS are among the prevalent species in Irish crops. Several BYDV detection methods, such as immunosorbent assays and PCR-based diagnostic tests, are available and routinely used. However, there are opportunities to develop improved assays to capture viral load information from different sample matrices. Here, we successfully developed a droplet digital PCR assay to detect and quantify BYDV-MAV and BYDV-PAS in both aphid and barley samples. The high specificity shown by this assay allows us to differentiate the two species from each other within a wide dynamic range. This assay will provide a better overview of the process underlying BYDV infection and transmission from the early stage of infection to the appearance of the symptoms.

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Journal of Plant Protection Research

Cited by 4 publications

References 13 publications

Two major quantitative trait loci control wheat dwarf virus resistance in four related winter wheat populations

Two major quantitative trait loci control wheat dwarf virus resistance in four related winter wheat populations

Development of loop-mediated isothermal amplification assay for rapid detection of genetically different wheat dwarf virus isolates

Development of a droplet digital PCR assay to detect and quantify BYDV-MAV and BYDV-PAS in their barley host and aphid vectors

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