The purpose of this study was to examine the relationships among the political leanings of a state, the state-level implicit and explicit biases against transgender people, and the presence of transgender athlete bans. The authors collected archival data from 2021 and 2022 bans in the United States to examine the hypotheses. As of 2022, 18 states had passed laws barring transgender athletes from participating in sports. Results from regression analyses showed that conservative-leaning states were more likely to enact transgender athlete bans than their peers. The relationship was mediated by explicit (but not implicit) bias toward transgender people. The findings have implications for research and practice. Sport managers have an opportunity to create transgender-inclusive workplaces for staff, coaches, and other managers. They should also work with campus counselors and other staff to ensure that transgender athletes have ample support.